中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2017, Vol. 46 ›› Issue (10): 869-873.doi: 10.12007/j.issn.0258‐4646.2017.10.002

• 论著 • 上一篇    下一篇

超声作用下2种压电陶瓷多孔支架材料上成骨细胞的增殖与分化

全泉1,矫义来2,曹洪娟1,吴琳1   

  1. 1 中国医科大学附属口腔医院修复一科,沈阳 110002;2 中国科学院金属研究所材料特种制备与加工研究部,沈阳 110016
  • 收稿日期:2016-12-15 出版日期:2017-10-30 发布日期:2017-10-16
  • 通讯作者: 吴琳 E-mail:wulin13@163.com
  • 作者简介:全泉(1984 -),女,主治医师,硕士
  • 基金资助:
    国家高技术研究发展计划(863计划)(2015AA033702);辽宁省自然科学基金(2014021008)

Effect of Low-intensity Pulsed Ultrasound on the Proliferation and Differentiation of Osteoblasts Cultured on Two Piezoelectric Ceramic Porous Scaffolds

QUAN Quan1,JIAO Yilai2,CAO Hongjuan1,WU Lin1   

  1. 1 Department of Prosthodontics,School of Stomatology,China Medical University,Shenyang 110002,China;2 Materials Fabrication and Processing Division,Institute of Metal Research,Chinese Academy of Sciences,Shenyang 110016,China
  • Received:2016-12-15 Online:2017-10-30 Published:2017-10-16

摘要: 目的 在低强度脉冲超声波(LIPUS)作用下对比研究钛酸钡/微弧氧化钛合金多孔材料(简称BaTiO3)和氧化锌/微弧氧 化钛合金多孔材料(简称ZnO)的表面MC3T3-E1成骨细胞增殖和分化的特性。方法 分别将BaTiO3和ZnO这2种材料随机分成超 声组(UBaTiO3组和UZnO组)和对照组(CBaTiO3组和CZnO组),将MC3T3-E1成骨细胞与材料复合培养,超声组每天接受20 min LIPUS辐照,对照组为不开功率源假辐照。通过检测噻唑兰(MTT)、碱性磷酸酶(ALP)和骨钙素(OCN )值观察成骨细胞增殖和 分化的水平。结果 4 d时UBaTiO3组MTT值高于CBaTiO3组,差异有统计学意义(P < 0.05);1、4和7 d时UBaTiO3组和CBaTiO3组的 MTT值均高于UZnO组和CZnO组,差异均有统计学意义(P < 0.05)。4和7 d时UBaTiO3组的ALP值高于CBaTiO3组,7 d时UZnO组的 ALP值高于CZnO组,差异均有统计学意义(P < 0.05);4和7 d时UBaTiO3组和CBaTiO3组的ALP值均高于UZnO组和CZnO组,差异均 有统计学意义(P < 0.05)。7、10和14 d时UBaTiO3组的OCN值高于CBaTiO3组,14 d时UZnO组的OCN值高于CZnO组,差异均有统 计学意义(P < 0.05),7、10和14 d时UBaTiO3组的OCN值高于UZnO组,10和14 d时CBaTiO3组的OCN值高于CZnO组,差异均有统计 学意义(P < 0.05)。结论 LIPUS可以促进 BaTiO3和ZnO这2种材料上MC3T3-E1的增殖与分化。在LIPUS作用下,BaTiO3材料优于 ZnO材料,更具有成为骨修复材料的潜能。

关键词: 低强度脉冲超声波, 成骨细胞, 压电陶瓷, 压电效应

Abstract: Objective To evaluate the effects of low-intensity pulsed ultrasound(LIPUS)treatment on the proliferation and differentia- tion of osteoblasts cultured on barium titanate/titanium alloy(BaTiO3)and zinc oxide/titanium alloy(ZnO)piezoelectric ceramic composite scaffolds. Methods The BaTiO3 and ZnO porous composite scaffolds were randomly divided into ultrasound(UBaTiO3 and UZnO)and control(CBaTiO3 and CZnO)groups,and MC3T3-E1 cells were cultured on the surface of the materials in each group. Cells in the ultra- sound groups were subjected to a 20-min treatment LIPUS session daily,and the control group was sham-treated without the power source. The proliferation and differentiation rates of osteoblasts were evaluated using methyl thiazolyl tetrazolium(MTT),alkaline phosphatase(ALP),and osteocalcin(OCN)assays. Results Significant differences in the MTT values were observed between the UBaTiO3 and CBa- TiO3 groups at day 4(P < 0.05)and between the UBaTiO3 and UZnO groups at days 1,4,and 7(P < 0.05). Compared with the CBaTiO3 group,the UBaTiO3 group showed significantly higher ALP activity and OCN levels at days 4 and 7(P < 0.05). Further,a significant difference in the ALP and OCN values between the UBaTiO3 and UZnO groups(P < 0.05)was observed. Conclusion LIPUS promotes the proliferation and differentiation of osteoblasts on BaTiO3 and ZnO scaffolds. BaTiO3 scaffolds enable better promotion of osteogenesis under LIPUS treatment than ZnO scaffolds do.

Key words: low-intensity pulsed ultrasound, osteoblast, piezoelectric ceramic, piezoelectric effect

中图分类号: 

  • R783.1
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