中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (6): 527-531.doi: 10.12007/j.issn.0258‐4646.2018.06.011

• 论著 • 上一篇    下一篇

脂肪源性干细胞促进大鼠神经施万细胞增殖和分泌功能

宋起滨1, 王哲2, 王宇令1, 李杨1, 林爽1   

  1. 1. 中国医科大学附属盛京医院整形美容科, 沈阳 110004;
    2. 中国医科大学附属盛京医院病理科, 沈阳 110004
  • 收稿日期:2017-10-13 出版日期:2018-06-30 发布日期:2018-05-29
  • 通讯作者: 宋起滨 E-mail:sqb_cmu2@163.com
  • 作者简介:宋起滨(1976-),男,主治医师,博士.
  • 基金资助:
    辽宁省博士启动基金(20131142)

Adipose-derived Stem Cells Promote Rat Schwann Cell Proliferation and Secretion of Nerve Regeneration Factors

SONG Qibin1, WANG Zhe2, WANG Yuling1, LI Yang1, LIN Shuang1   

  1. 1. Department of Plastic Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China;
    2. Department of Pathology, Shengjing Hospital, China Medical University, Shenyang 110004, China
  • Received:2017-10-13 Online:2018-06-30 Published:2018-05-29

摘要: 目的 检测脂肪源性干细胞(ADSCs)对大鼠神经施万细胞增殖及分泌功能的影响。方法 从SD大鼠腹股沟区脂肪中提取ADSCs,通过流式细胞仪检测表面抗体标记物。获取ADSCs条件培养基,通过ELISA判断ADSCs条件培养基中神经再生相关因子[神经生长因子(NGF)、脑源性神经营养因子(BDNF)、胶质细胞源性神经营养因子(GDNF)和血管内皮生长因子(VEGF)]的含量。分别将大鼠神经施万细胞与ADSCs条件培养基及ADSCs共培养,通过MTT观察ADSCs条件培养基及ADSCs对大鼠神经施万细胞增殖的影响,进一步通过Western blotting和实时PCR分别检测ADSCs条件培养基及ADSCs作用于大鼠神经施万细胞后神经再生相关因子蛋白和mRNA水平的改变。结果 细胞表面抗体标记物检测发现CD44和CD90阳性,CD31和CD45阴性,证明ADSCs提取成功。ELISA检测发现ADSCs条件培养基中含有大量神经再生相关因子。MTT检测发现ADSCs条件培养基及ADSCs均可以促进大鼠神经施万细胞的增殖。Western blotting和实时PCR结果证明ADSCs条件培养基及ADSCs可以促进大鼠神经施万细胞神经再生相关因子的表达。结论 ADSCs可以促进大鼠神经施万细胞增殖及神经再生相关因子分泌。

关键词: 脂肪源性干细胞, 施万细胞, 增殖, 神经再生相关因子

Abstract: Objective To detect the effects of adipose-derived stem cells(ADSCs) on proliferation of rat Schwann cells and secretion of nerve regeneration-related factors from Schwann cells. Methods ADSCs were extracted from Sprague Dawley rats, and surface antibody markers were identified by flow cytometry. ADSC-conditioned medium was obtained and the contents of nerve regeneration-related factors (NGF, BDNF, GDNF, and VEGF) in the medium were determined by enzyme-linked immunosorbent assay. Schwann cells were incubated either with the ADSC-conditioned medium or with ADSCs. The effect of the medium and ADSCs on the proliferation of Schwann cells was observed by MTT. The levels of nerve regeneration-related factors(NGF, BDNF, GDNF, and VEGF) in Schwann cells were detected by Western blotting and real-time polymerase chain reaction. Results The evaluation of cell surface antibody markers revealed CD44 and CD90 positivity and CD31 and CD45 negativity, indicating that the extraction of ADSCs was successful. Enzyme-linked immunosorbent assay revealed that the ADSC-conditioned medium contained an elevated amount of nerve regeneration-related factors. MTT assay showed that the ADSC-conditioned medium and ADSCs can promote the proliferation of Schwann cells. Western blotting and real-time polymerase chain reaction showed that ADSC-conditioned medium and ADSCs could promote the expression of nerve regeneration-related factors in Schwann cells. Conclusion ADSCs can promote Schwann cell proliferation and secretion of nerve regeneration factors.

Key words: adipose derived stem cells, Schwann cells, proliferation, nerve regeneration factor

中图分类号: 

  • R329.21
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