中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (6): 548-551.doi: 10.12007/j.issn.0258‐4646.2018.06.015

• 论著 • 上一篇    下一篇

PP242对人晶状体上皮细胞凋亡的诱导及凋亡相关蛋白Bax表达的影响

白雪, 宁宏   

  1. 中国医科大学附属第一医院眼科, 沈阳 110001
  • 收稿日期:2017-12-07 出版日期:2018-06-30 发布日期:2018-05-29
  • 通讯作者: 宁宏 E-mail:xiaoxiao1998@21cn.com
  • 作者简介:白雪(1986-),女,医师,硕士.
  • 基金资助:
    辽宁省社会发展攻关计划(2013225303)

Effect of PP242 on Apoptosis and Expression of the Apoptosis Protein Bax in Human Lens Epithelial Cells

BAI Xue, NING Hong   

  1. Department of Ophthalmology, The First Hospital, China Medical University, Shenyang 110001, China
  • Received:2017-12-07 Online:2018-06-30 Published:2018-05-29

摘要: 目的 研究PP242对人晶状体上皮细胞(HLECs)凋亡的诱导及凋亡蛋白Bax表达的影响。方法 用不同浓度PP242处理人晶状体上皮细胞系SRA01/04,分别用浓度为0、250、500、750、1 000 nmol/L的PP242处理HLECs 48 h后,Annexin V/PI双染流式细胞仪检测细胞凋亡率;采用实时定量PCR及Western blotting方法检测Bax的mRNA及蛋白的表达。结果 250、500、750和1 000 nmol/L PP242对人晶状体上皮细胞系SRA01/04作用48 h后,早期凋亡率分别为7.20%±0.36%、13.77%±0.21%、16.31%±0.20%和18.67%±0.49%,与对照组(0 nmol/L)差异有统计学意义(P<0.05)。Western blotting结果显示,随着PP242浓度的增加,Bax蛋白表达量增加,与对照组(0 nmol/L)比较差异有统计学意义(P<0.05);实时定量PCR结果显示,Bax mRNA表达量随PP242药物浓度的增加而增加,相对定量mRNA结果分别为2.157±0.125、3.733±0.302、5.596±0.261和7.436±0.167,与对照组(0 nmol/L)比较差异有统计学意义(P<0.05)。结论 PP242可以诱导HLECs的凋亡,增加其凋亡蛋白Bax的表达。

关键词: PP242, 人晶状体上皮细胞, Bax

Abstract: Objective To study the effect of PP242 on apoptosis and the expression of the apoptosis protein Bax in human lens epithelial cells(HLECs). Methods Immortal HLECs SRA01/04 were cultured and treated with different concentrations(0, 250, 500, 750, or 1 000 nmol/L) of PP242. Forty-eight hours after PP242 treatment, the apoptotic rate of cells in each group was examined with flow cytometry(FCA, Annexin V + PI staining). Using reverse transcription quantitative polymerase chain reaction and Western blotting, the mRNA and protein levels of Bax were examined. Results Forty-eight hours after treatment with 250, 500, 750, or 1 000 nmol/L PP242, the apoptotic rate of HLECs was 7.20%±0.36%, 13.77%±0.21%, 16.31%±0.20%, or 18.67%±0.49%, respectively. Apoptotic rate in the control group was significantly different(P<0.05) than that in the treatment groups. Results of the Western blotting revealed that the level of Bax was significantly increased. Further, the results of reverse-transcription quantitative PCR showed that expression of Bax mRNA increased in the treatment groups. The relative quantification of Bax mRNA was 2.157±0.125, 3.733±0.302, 5.596±0.261, 7.436±0.167. Compared with the control group, the difference was statistically significant(P<0.05). Conclusion PP242 induces the expression of Bax and promotes apoptosis in cultured HLECs.

Key words: PP242, human lens epithelial cells, Bax

中图分类号: 

  • R776.1
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