中国医科大学学报

中国医科大学学报

中国医科大学学报

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白色念珠菌感染对脾虚模型小鼠T细胞亚群的影响

马贤德1,张威2,关洪全3   

  1. 辽宁中医药大学1. 教学实验中心生物技术实验室;2. 附属医院神经内科;3. 基础医学院免疫病原生物教研室,沈阳110847
  • 收稿日期:2015-10-27 出版日期:2015-12-15 发布日期:2015-12-17
  • 通讯作者: 关洪全,E-mail:hongquanguan@sina.com
  • 作者简介:马贤德(1979 -),男,实验师,博士研究生.
  • 基金资助:

    国家自然科学基金(81173145)

Influence ofCandida albicans Infection on T Cell Subsets in theMice with Spleen Deficiency

MA Xiande1,ZHANG Wei2,GUAN Hongquan3   

  1. 1. Biology Technology Lab,Teaching Experiment Center,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;2. Department of Internal Medicine-neurology,Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang110847,China 3.Department of Immunology and Pathogenic Biology,Basic Medical College,Liaoning University of Traditional Chinese Medicine,Shenyang110847,China
  • Received:2015-10-27 Online:2015-12-15 Published:2015-12-17

摘要:

目的 探讨白色念珠菌感染后脾虚小鼠T细胞亚群的变化及其免疫机制。方法 将健康SPF级昆明种小白鼠150只随机分为空白对照组(N1组,n = 30)、空白感染白念组(N2组,n = 40)、脾虚模型对照组(M1组,n = 40)、脾虚感染白念组(M2组,n = 40)。N2和M2组小鼠经口感染白色念珠菌(2×108 CFU/mL,0.2 mL/10 g体质量)。分别于染菌第7、14、21天,从各组小鼠中随机抽取10只,检测小鼠肠黏膜固有层中CD4+/CD8+T细胞百分比、小肠组织中干扰素γ(IFN?γ)和白细胞介素4(IL-4)mRNA及蛋白的表达水平。结果 与N1组比较,N2组、M1组及M2组小鼠小肠黏膜固有层中CD4+T细胞比例下降(P < 0.01),CD8+T细
胞比例上升(P < 0.01),CD4/CD8比值显著下降(P < 0.01),IFN-γ、IL-4 mRNA及蛋白的表达水平上调(P < 0.01)。IFN-γ以M2组上调最为明显,而IL-4则以N2组上调最为明显。结论 脾虚小鼠对白色念珠菌的易感性增加,感染的发生机制可能与机体自身对Th1/Th2平衡的调节密切相关。

关键词: 脾虚证, 白色念珠菌, 感染, T细胞亚群

Abstract:

Objective To observe the influence ofCandida albicans infection on T cell subsets in mice with spleen deficiency,so as to explore theimmune mechanism. Methods Totally 150 healthy SPF mice were randomly divided into four groups:control group(N1 group,n = 30);Candida albicans infection group(N2,n = 40),spleen deficiency model group(M1,n = 40),spleen deficiency model combined withCandida albicans infection group(M2,n = 40). N2 and M1 mice were infected by Candida albicans at a concentration of 2×108 CFU/mL. Ten mice were randomly selected from each group at 7,14 and 21 days after infection respectively,and the index was detected. Flow cytometry was used to detect the percentage of CD4+/CD8+T cells in intestinal mucosa of mice,and the expression level of IL-4 and IFN-γ mRNA was detected by RT-PCR assay. The levels of IFN-and IL-4 were detected by Western blot assay. Results Compared with N1 group,the proportion of CD4+T cells in the lamina of the natural layer of the small intestine was decreased(P <0.01) the proportion of CD8+T cells was increased(P <0.01),the ratio of the two was significantly decreased(P < 0.01),and the expression levels of IL-4,mRNA IFN?γ and protein in the small intestine tissues were increased in other groups (P < 0.01). In addition,the expression level of IFN-γ was significantly increased in M2 group,while the expression of IL-4 was significantly increased in N2 group. Conclusion The susceptibility ofCandida albicans infection was increased in spleen deficiency mice,which may be closely related to the regulation of Th1/Th2 balance.

Key words: spleen deficiency, Candidaalbicans, infection, T lymphocyte subsets

中图分类号: 

  • R392.9
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