中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2016, Vol. 45 ›› Issue (8): 708–710.doi: 10.12007/j.issn.0258-4646.2016.08.009

• 论著 • 上一篇    下一篇

大鼠创伤性脑损伤后精神障碍的精神行为学改变

李力卓 1,2,何松柏 2   

  1. 1 首都医科大学宣武医院急诊科,北京 100053;2 中国医科大学附属盛京医院急诊科,沈阳 110004
  • 收稿日期:2016-01-06 出版日期:2016-08-20 发布日期:2016-07-23
  • 作者简介:李力卓(1971 -),男,副教授,博士 .
  • 基金资助:
    辽宁省自然科学基金(2013010083?401);沈阳市科学技 术计划(F13?220?9?53)

Spirit Behavioral Changes of Mental Disorders Caused by Traumatic Brain Injury in Rats

LI Lizhuo1,HE Songbai2   

  1. 1 Department of Emergency,Xuanwu Hospital,Capital Medical University,Beijing 100053,China;2 Department of Emergency,Shengjing Hospital,China Medical University,Shenyang 110004,China
  • Received:2016-01-06 Online:2016-08-20 Published:2016-07-23

摘要: 目的 利用改良 FEENEY’S 法建立大鼠创伤性脑损伤(TBI)模型,探讨 TBI 后精神障碍的精神行为学改变。方法 将成年雄性 SD 大鼠随机分为正常对照组、TBI 组和假手术组,每组各 10 只。采用改良 FEENEY’S 法建立大鼠 TBI 模型。TBI 后 15 d开始,分别依次进行旷场实验(OFT)和强迫游泳实验(FST)。记录 OFT 中大鼠 5 min 内的移动总距离(DMT)、平均速度(VM),记录 FST 中大鼠 5 min 内不动时间总和(ITT),观察大鼠的高警觉性、焦虑/抑郁和恐惧行为的变化。结果 TBI 组大鼠的 DMT[(1 228.10 ± 204.97)cm]、运 动 VM[(4.09 ± 0.68)cm/s]较 假 手 术 组[(1 660.01 ± 332.09)cm、(5.53 ± 1.10)cm/s]和 正 常 对 照 组[(2 022.32±280.92)cm、(7.28±1.80)cm/s]均明显减少,差异均有统计学意义(P < 0.05)。TBI 组大鼠的 ITT[(149.88±27.36)s]较假手术组[(120.55±22.31)s]、正常对照组[(106.10±29.03)s]明显增加,差异有统计学意义(P < 0.05)。结论 TBI 后,大鼠出现了焦虑/抑郁样精神行为异常。

关键词: 创伤性脑损伤, 精神障碍, 旷场实验, 强迫游泳实验

Abstract: Objective To set up traumatic brain injury(TBI)in rats by the improved device of FEENEY’s weight dropping model and to explore the spirit behavioral changes of mental disorders caused by TBI. Methods SD rats were randomly divided into normal control group(normal group,n = 10),traumatic brain injury group(TBI group,n = 10)and sham group(n = 10). The improved device of FEENEY’s method was adopted to establish rat TBI model. For the following 15 days after TBI,open-field(OF)and forced swimming(FS)tests were carried out and the behavior of the TBI rats within 5 minutes were recorded,the behavioral indexes,such as distance moved tota(l DMT),velocity mean(VM)and immobility time total(ITT),were analyzed. With above experiments,rats' fear response to environment,high alertness and anxiety and depression behavior were evaluated. Results DMT and VM of TBI group rats(1 228.10±204.97 cm,4.09±0.68 cm/s)were significantly lower than those of the normal group(2 022.32±280.92 cm,7.28±1.80 cm/s)or sham group(1 660.01±332.09 cm,5.53±1.10 cm/s)(P < 0.05).The ITT of TBI group(149.88±27.36 s,P < 0.05)was longer than that of the normal group(106.10±29.03 s)or sham group(120.55±22.31 s)significantly(P < 0.05).Conclusion TBI leads to rats' abnormal emotional and behavioral response.

Key words: traumatic brain injury, mental disorders, open-field test, forced swimming tests

中图分类号: 

  • R749.1+2
[1] 黄海能, 罗起胜, 赵邦, 谭源福, 邓元央, 黄华东, 符黄德, 李传玉. 兔脑创伤后早期血清β淀粉样前体蛋白动态变化的实验研究[J]. 中国医科大学学报, 2010, 39(1): 22-.
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