中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2016, Vol. 45 ›› Issue (8): 711–714.doi: 10.12007/j.issn.0258-4646.2016.08.010

• 论著 • 上一篇    下一篇

DEK 蛋白在膀胱尿路上皮癌癌组织和尿液中的表达及临床意义

吴琼 1,王贻兵 2,杨腾飞 1   

  1. 中国医科大学附属盛京医院 1 涉外医疗服务中心;2 泌尿外科,沈阳 110004
  • 收稿日期:2016-03-29 出版日期:2016-08-20 发布日期:2016-07-23
  • 作者简介:吴琼(1972 -),女,主治医师,博士 .
  • 基金资助:
    辽宁省博士科研启动基金(20121129)

Expression of DEK Protein in the Tissue and Voided Urine of Patients with Bladder Urothelial Carcinoma and Its Clinical Significance

WU Qiong1,WANG Yibing2,YANG Tengfei1   

  1. 1 International Medical Service Center,Shengjing Hospital,China Medical University,Shenyang 110004,China;2 Department of Urology,Shengjing Hospital,China Medical University,Shenyang 110004,China
  • Received:2016-03-29 Online:2016-08-20 Published:2016-07-23

摘要: 目的 探究 DEK 蛋白在膀胱尿路上皮癌癌组织和尿液中的表达及其与肿瘤病理分期之间的关系。方法 应用免疫组化技术检测 48 例膀胱尿路上皮癌组织和 18 例癌旁正常组织 DEK 蛋白的表达,应用蛋白免疫印迹的方法检测 28 例膀胱尿路上皮癌患者和 6 例健康者(对照组)尿中的 DEK 蛋白的表达。结果 膀胱尿路上皮癌癌组织中 DEK 蛋白的阳性表达率(73%)高于癌旁正常组织(33%,P < 0.05);浅表性膀胱尿路上皮癌组织中 DEK 的阳性表达率(86%)高于浸润性(55%,P < 0.05);膀胱尿路上皮癌患者尿液中 DEK 相对表达量(0.834)明显高于对照组(0.242,P < 0.05);以对照组尿液中 DEK 的相对表达量作为标准,诊断膀胱尿路上皮癌的灵敏度可达 82.1%。结论 DEK 蛋白在膀胱尿路上皮癌患者癌组织和尿液中呈现阳性表达,与膀胱癌的病理分级有密切关系。癌症早期 DEK 蛋白在组织中的阳性表达高于癌症晚期。组织中和尿液中 DEK 表达认为是潜在的诊断膀胱尿路上皮癌的肿瘤标记物。

关键词: 膀胱尿路上皮癌, DEK 蛋白, 癌组织, 尿液

Abstract: Objective To detect the presence of DEK protein in the tissue and voided urine of patients with bladder urothelial carcinoma,and analyze the relationship between DEK protein and clinical pathological classification of bladder cancer. Methods The expression of DEK protein was examined by immunohistochemistry in 48 bladder cancer tissues and 18 adjacent normal tissues(control group). The presence of DEK protein in voided urine was analyzed by western blot in 28 bladder cancer patients and 6 healthy individuals(control group). Results The positive expression of DEK protein in bladder tumor tissues(73%)is higher than in adjacent normal tissues(33%,P < 0.05). The expression of DEK in superficial bladder cancer(86%)was found higher than invasive bladder cancer(55%)with significant differences(P < 0.05). The expression of DEK in urine samples of bladder cancer patients(0.834)was found higher than control group(0.242,P < 0.05);Compared with the DEK expression of control group,the sensitivity of diagnosis of bladder cancer with voided urine could be 82.1%. Conclusion DEK protein positive expressed in tissues and voided urine of patients with bladder urothelial carcinoma. The expression was correlated with pathological classification of bladder cancer. The positive rate of DEK expression in early stage of tumor tissues is higher than late stage. The presence of DEK protein in tissues and voided urine can be considered as a suitable biomarker for bladder cancer potentially.

Key words: bladder urothelial carcinoma, DEK protein, cancer tissue, voided urine

中图分类号: 

  • R737.14
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