中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2016, Vol. 45 ›› Issue (8): 728–732.doi: 10.12007/j.issn.0258-4646.2016.08.014

• 论著 • 上一篇    下一篇

咪唑立宾对单侧输尿管梗阻小鼠肾小管上皮间质转化的影响

于方,邓海月,姜红   

  1. 中国医科大学附属第一医院儿科,沈阳 110001
  • 收稿日期:2015-11-12 出版日期:2016-08-20 发布日期:2016-07-23
  • 通讯作者: 姜红 E-mail:jianghong724@163.com
  • 作者简介:于方(1992 -),女,硕士研究生 .
  • 基金资助:
    高等学校博士学科点专项科研基金(20122104110001)

Effects of Mizoribine on Renal Tubular Epithelial-mesenchymal Transition in Unilateral Ureteral Obstruction Mice

YU Fang,DENG Haiyue,JIANG Hong   

  1. Department of Pediatrics,The First Hospital,China Medical University,Shenyang 110001,China
  • Received:2015-11-12 Online:2016-08-20 Published:2016-07-23

摘要: 目的 观察咪唑立宾(MZR)对单侧输尿管梗阻(UUO)小鼠肾小管上皮间质转化(EMT)的影响,探讨其抗肾间质纤维化机制。方法 将 24 只 CD1 小鼠随机分为假手术组、UUO 模型组和 MZR 治疗组,每组 8 只。造模前 1 d ,MZR 治疗组给予 MZR[10 mg(/ kg?d)]灌胃,假手术组、UUO 模型组给予等量生理盐水灌胃。术后 14 d 收集小鼠血液,记录血肌酐(Scr)和尿素氮(BUN);收集梗阻侧肾脏,HE 染色、Masson 染色观察肾脏病理改变;免疫组化、Western blot 检测 α-平滑肌肌动蛋白(α-SMA)和 E-钙黏蛋白(E-Cad)在肾脏的表达。结果 与假手术组相比,UUO 模型组血肌酐、尿素氮显著升高,肾组织病理改变明显,肾组 织中 α-SMA 表达增加而 E-Cad 表达减少,差异有统计学意义(P < 0.05);与 UUO 模型组相比,MZR 治疗组 Scr、BUN 及肾组织病 理改变均不同程度改善,肾组织中 α-SMA 表达受抑而 E-Cad 表达上调,差异有统计学意义(P < 0.05)。结论 MZR 可抑制 UUO 小鼠肾小管 EMT 的发展,减轻肾小管间质纤维化程度,改善肾功能。

关键词: 单侧输尿管梗阻, 上皮间质转化, 咪唑立宾

Abstract: Objective To observe the effects of mizoribine(MZR)on renal tubular epithelial - mesenchymal transition(EMT)of mice which have been performed unilateral ureteral obstruction(UUO),and study the mechanism of its anti-fibrosis of renal interstitial. Methods A total of 24 CD1 mice were randomly divided into sham group,UUO model group and MZR treatment group,with 8 mice in each group. The day before operation,mice of MZR treatment group had been given MZR 10 mg/kg/d lavage,those of sham group and UUO model group had been given equal saline lavage. Fourteen days after the operation,blood was collected and serum creatinine and blood urea nitrogen were measured;the obstruction kidneys were harvested for section,HE staining and Masson staining were employed to observe the changes of kidney pathological;the expression of α -SMA and E-Cad in kidney with detected by immunohistochemical and Western blot method. Results Compared with sham group,serum creatinine and blood urea nitrogen of mice in UUO model group and MZR treatment group were significantly elevated,kidney pathological changes and the expression of α-SMA in renal tissue were increased and that of E-Cad was reduced,the differences were all statistically significant(P <0.05);compared with UUO model group,mice in MZR treatment group had different degree of improvements in serum creatinine,blood urea nitrogen and kidney pathological changes,the expression of α-SMA in renal tissue was inhibited and that of E-Cad was increases,and the differences were statistically significant(P < 0.05). Conclusion MZR may inhibit the development of renal tubular EMT in UUO mice,thereby reduce the level of renal tubule interstitial fibrosis and improve renal function.

Key words: unilateral ureteral obstruction, epithelial-mesenchymal transition, mizoribine

中图分类号: 

  • R692.6
[1] 孙洪利. 小鼠肝硬化-肝癌上皮间质转化的研究[J]. 中国医科大学学报, 2018, 47(2): 157-161.
[2] 崔苗苗,宋月佳,顾云鹤,戚基萍. 上皮间质转化在胃癌及相应转移淋巴结中的表达及意义[J]. 中国医科大学学报, 2015, 44(5): 438-442.
[3] 刘蕾,肖伟. Notch基因胞内活化部分真核表达载体p3XFLAG?CMV7?NICD1的构建与鉴定[J]. 中国医科大学学报, 2015, 44(3): 217-220.
[4] 王文双, 杨兴升, 马先莹, . Twist介导的上皮间质转化在卵巢癌组织中的作用[J]. 中国医科大学学报, 2012, 41(2): 175-177.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!

中国医科大学学报版权所有©2018

未经允许,严禁擅自转载本站图文资料

地址:中国 沈阳市沈北新区蒲河路77号 110122

辽ICP备05014850

JOURNAL OF CHINA MEDICAL UNIVERSITY

ADDRESS: NO.77 PUHE ROAD

SHENYANG NORTH NEW AREA, SHENYANG

LIAONING PROVINCE, P.R. CHINA