中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2016, Vol. 45 ›› Issue (8): 742–745.doi: 10.12007/j.issn.0258-4646.2016.08.017

• 论著 • 上一篇    下一篇

ω-6 大豆油脂肪乳对小鼠腹泻的保护作用

孙明立,赵海山,姚维范,魏敏杰   

  1. 中国医科大学药学院药理教研室,沈阳 110122
  • 收稿日期:2015-12-09 出版日期:2016-08-20 发布日期:2016-07-23
  • 通讯作者: 魏敏杰 E-mail:weiminjie1963@163.com
  • 作者简介:孙明立(1980 -),男,讲师,博士 .

Protective Effect of ω-6 Soybean Oil Fat Emulsion on Folium Sennae-induced Diarrhea in Mice

SUN Mingli,ZHAO Haishan,YAO Weifan,WEI Minjie   

  1. Department of Pharmacology,School of Pharmacy,China Medical University,Shenyang 110122,China
  • Received:2015-12-09 Online:2016-08-20 Published:2016-07-23

摘要: 目的 探讨 ω-6 大豆油脂肪乳对番泻叶导致的小鼠腹泻的保护作用。方法 将 36 只昆明小鼠随机分为对照组、腹泻组和 ω-6 大豆油脂肪乳组,每组 12 只。利用番泻叶制备小鼠腹泻模型。造模后,ω-6 大豆油脂肪乳组注射 ω-6 大豆油脂肪乳(15mL/kg,每日 1 次),对照组与腹泻组小鼠每日尾静脉注射等剂量生理盐水,连续给药 10 d。自造模之日起动态观察小鼠一般状态并记录小鼠体质量;并于给药前及给药第 1、4、7 和 10 天分别计算小鼠腹泻指数;给药 10 d 后处死小鼠,采用 HE 染色法观察小鼠小肠黏膜形态学变化;采用生化法检测小鼠血浆白蛋白(ALB)水平;采用免疫组化检测小鼠小肠黏膜组织中增殖细胞核抗原(PCNA)的表达。结果 与腹泻组相比,ω-6 大豆油脂肪乳组小鼠的一般状况和体质量明显改善,小鼠腹泻指数明显降低;给药 10 d 后,与腹泻组相比,ω-6 大豆油脂肪乳组小鼠小肠黏膜损伤明显改善、血浆 ALB 水平显著升高,小肠黏膜中 PCNA 表达显著升高(P < 0.05)。结论 ω-6 大豆油脂肪乳对番泻叶所致的小鼠腹泻具有显著的保护作用,其机制可能与上调 PCNA 表达水平有关。

关键词: ω-6 大豆油脂肪乳, 番泻叶, 腹泻, 小肠黏膜损伤

Abstract: Objective To investigate the protective effect of ω-6 soybean oil fat emulsion on folium sennae-induced diarrhea in mice. Methods Thirty-six Kunming mice were randomly divided into three groups,including control,diarrhea,ω-6 soybean oil fat emulsion group(12 mice in each group). Besides the mice in control group,other mice were administrated folium sennae by gavage for 15 days to establish the diarrhea model. Then mice in ω-6 soybean oil fat emulsion group received ω-6 soybean oil fat emulsion by intravenously administration at a dose of 15 mL/kg daily since 6th day after intragastric administration of folium sennae for 10 days. Animals in control group and diarrhea group were intravenously administered with same volume of saline. The body weight,general state and diarrhea index of the mice in each group were dynamically assessed. Ten days after intravenous injection,mice in every group were sacrificed and tissues were collected. Morphology of intestine mucosa was observed after HE staining. Albumin(ALB)level in plasma was evaluated by biochemical method. Proliferating cell nuclear antigen(PCNA)expression in intestine mucosa were assessed by immunohistochemical method. Results Compared with that in the diarrhea group,the general status,body weight and diarrhea index of mice in ω-6 soybean oil fat emulsion group were improved significantly. Ten days after intravenously administration,pathological change in intestine mucosa of mice in ω-6 soybean oil fat emulsion group was improved significantly,ALB level in plasma and PCNA expression in intestine mucosa were significantly increased(P < 0.05)compared with that in diarrhea group. Conclusion ω-6 soybean oil fat emulsion has a significant protective effect on the diarrhea of mice induced by folium sennae,which may be related to the up-regulated expression of PCNA by ω-6 soybean oil fat emulsion.

Key words: ω-6 soybean oil fat emulsion, folium sennae, diarrhea, intestinal mucosal injury

中图分类号: 

  • R965.1
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