中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2016, Vol. 45 ›› Issue (12): 1063–1065.doi: 10.12007/j.issn.0258-4646.2016.12.002

• 论著 • 上一篇    下一篇

3 种小鼠晶状体组织石蜡切片固定液的比较

于紫燕,王春霞,孙琦,赵江月,张劲松   

  1. 中国医科大学附属第四医院眼科,辽宁省晶状体学重点实验室,沈阳 110005
  • 收稿日期:2016-04-27 出版日期:2016-12-28 发布日期:2016-12-06
  • 通讯作者: 赵江月 E-mail:zhaojiangyue@hotmail.com
  • 作者简介:于紫燕(1981 -),女,主治医师,博士 .
  • 基金资助:
    辽宁省自然科学基金(201602872);沈阳市科学技术计划(F13?318?1?09)

Comparison of Fixation Effects of Three Different Complex Fixatives on Mouse Lens Tissue

YU Ziyan,WANG Chunxia,SUN Qi,ZHAO Jiangyue,ZHANG Jinsong   

  1. Department of Ophthalmology,The Fourth Affiliated Hospital,China Medical University,Lens Science Research Key Laboratory of Liaoning Province,Shenyang 110005,China
  • Received:2016-04-27 Online:2016-12-28 Published:2016-12-06

摘要: 目的 比较 3 种固定液对小鼠晶状体组织石蜡切片的影响,优化小鼠晶状体组织石蜡切片固定方法。方法 选取 3 种 常用固定液(改良 Davison’s 固定液、传统 Davison’s 固定液和 10%中性甲醛固定液),固定小鼠晶状体组织,并制成石蜡切片,经 HE 染色后在显微镜下观察。结果 传统 Davison’s 固定液固定的晶状体组织切片无碎裂,改良 Davison’s 固定液固定的晶状体 组织中央区碎裂。改良 Davison’s 固定液和传统 Davison’s 固定液固定的小鼠眼球外形均无明显变形和收缩,未见明显的视网 膜脱离,镜下视网膜组织结构完整清晰,各层细胞排列紧密,无明显裂隙,染色鲜艳。采用 10%中性甲醛固定液固定的小鼠眼球 壁组织发生严重皱缩,晶状体组织皱缩,空隙和空泡形成。结论 传统 Davison’s 固定液固定的小鼠眼球晶状体组织石蜡切片 质量效果优于改良 Davison’s 固定液和 10%中性甲醛固定液。

关键词: 小鼠眼球, 石蜡切片, 固定液, 晶状体

Abstract: Objective To compare the effects of three kinds of fixative solutions on paraffin section of mouse lens tissue and optimize the fixing method of paraffin section in mouse lens tissue. Methods Three kinds of conventional fixatives were selected for the test,including the conventional Davison’s solution,modified Davison’s solution and 10% neutral buffered formalin. Mice eyeball tissues were fixed with three different fixa- tives,embedded,sliced and then stained with HE method. The paraffin slices were observed under the light microscope. Results The structures of lens and retina fixed in conventional Davison’s fixative solutions were clear and intact,and the cells were arranged regularly and compactly. There was no eyeball distortion,contraction and retinal detachment in the eyeballs fixed in modified and conventional Davison’s fixative solution. However,the ones fixed in 10% neutral buffered formalin showed eyeball distortion and contraction,space and spherules. Conclusion The mice lens slides made from tissues fixed by conventional Davison’s fixative solution are better than fixed by modified Davison’s fixative solution and the 10% neutral buffered formalin fixed ones.

Key words: mouse eyeball, paraffin slice, fixative solution, lens

中图分类号: 

  • R36
[1] 白雪, 宁宏. PP242对人晶状体上皮细胞凋亡的诱导及凋亡相关蛋白Bax表达的影响[J]. 中国医科大学学报, 2018, 47(6): 548-551.
[2] 白雪,冯浩,张柳,杨志博,宁宏. PP242 对人晶状体上皮细胞凋亡相关蛋白 Bcl-2 表达的影响[J]. 中国医科大学学报, 2016, 45(4): 324-327.
[3] 于紫燕 ,王春霞 ,孙琦 ,赵江月 ,,张劲松. Msx2 条件性基因敲除小鼠动物模型的建立和表型的初步研究[J]. 中国医科大学学报, 2016, 45(11): 964-967.
[4] 马立威,张小霞,周文凯,石栋,徐静娴,张劲松. 波前像差对术后晶状体后囊膜混浊诊治的临床意义[J]. 中国医科大学学报, 2016, 45(10): 891-894.
[5] 韩笑,王欣玲,吴迪,张劲松,阎启昌. SUMO蛋白在体外培养的人晶状体上皮细胞内的表达及对氧化应激的调控作用[J]. 中国医科大学学报, 2015, 44(3): 193-198.
[6] 刘加勇,王思敏,林婷婷,冯浩,宁宏. GRIM?19在H2O2诱导的晶状体上皮细胞氧化应激中的表达[J]. 中国医科大学学报, 2014, 43(4): 337-340.
[7] 赵琪,赵江月,张劲松. 骨形成蛋白受体ⅠA在晶状体内条件敲除后对胚眼晶状体发育的影响[J]. 中国医科大学学报, 2014, 43(3): 209-212.
[8] 冯浩,林婷婷,王思敏,刘加勇,宁宏. PP242对人晶状体上皮细胞增殖的抑制作用及GRIM-19表达的影响[J]. 中国医科大学学报, 2013, 42(5): 416-419.
[9] 赵江月,王静,孙琦,张劲松. Toric人工晶状体植入术矫正高度角膜散光的视觉质量[J]. 中国医科大学学报, 2013, 42(11): 1022-1024.
[10] 马立威,张雪岩,许军,张劲松. 不同损伤条件下热休克蛋白27在人晶状体上皮细胞中的表达[J]. 中国医科大学学报, 2012, 41(9): 795-797.
[11] 王建平,张梅英,勾文峰,高野康雄,郑华川. 晶状体自发肿瘤转基因鼠脑横截面标本制备及鉴定 [J]. 中国医科大学学报, 2012, 41(8): 673-675.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!

中国医科大学学报版权所有©2018

未经允许,严禁擅自转载本站图文资料

地址:中国 沈阳市沈北新区蒲河路77号 110122

辽ICP备05014850

JOURNAL OF CHINA MEDICAL UNIVERSITY

ADDRESS: NO.77 PUHE ROAD

SHENYANG NORTH NEW AREA, SHENYANG

LIAONING PROVINCE, P.R. CHINA