中国医科大学学报

中国医科大学学报

中国医科大学学报 ›› 2016, Vol. 45 ›› Issue (12): 1100–1104.doi: 10.12007/j.issn.0258-4646.2016.12.010

• 论著 • 上一篇    下一篇

经典瞬时受体电位通道蛋白在 β 淀粉样蛋白诱导的阿尔茨海默病 小鼠海马区的表达

张改改 1,张丽艳 1,邹丹 1,沈薇 1,金戈 2,张利华 1   

  1. 沈阳医学院基础医学院 1病理生理教研室;2药理学教研室,沈阳 110034
  • 收稿日期:2016-09-21 出版日期:2016-12-28 发布日期:2016-12-06
  • 通讯作者: 张丽艳 E-mail:1580471013@qq.com
  • 作者简介:张改改(1984 -),女,硕士研究生 .
  • 基金资助:
    辽宁省科技厅科学事业公益研究基金(2014001024);高等学校国家级、省级 大学生创新创业训练计划(201410164017,201410164000017)

Expression of Canonical Transient Receptor Potential Channel Protein in the Hippocampus of Amyloid β Protein-induced Alzheimer’s Disease Mice

ZHANG Gaigai1,ZHANG Liyan1,ZOU Dan1,SHEN Wei1,JIN Ge2,ZHANG Lihua1   

  1. 1Department of Pathophysiology,College of Basic Medical Sciences,Shenyang Medical College,Shenyang 110034,China;2 Department of Pharmacology,College of Basic Medical Sciences,Shenyang Medical College,Shenyang 110034,China
  • Received:2016-09-21 Online:2016-12-28 Published:2016-12-06

摘要: 目的 探讨经典瞬时受体电位(TRPC)通道蛋白在阿尔茨海默病(AD)小鼠海马区中的表达情况。方法 将 36 只 ICR 小 鼠随机分为 AD 组和对照组,每组 18 只。β 淀粉样蛋白(Aβ)1-42 侧脑室注射制作 AD 小鼠模型。Morris 水迷宫试验测定小鼠学习记忆能力。逆转录聚合酶链反应(RT-PCR)检测海马区 TRPC1~TRPC7 mRNA 的表达。分别采用免疫荧光和 Western blotting 检测海马区的 TRPC4 蛋白表达。结果 AD 组小鼠水迷宫测试的逃避潜伏期较对照组明显延长(P < 0.01),在目标象限停留时 间明显缩短(P < 0.01),穿越平台次数明显减少(P < 0.01)。RT-PCR 结果显示,在 AD 组及对照组 TRPC1~TRPC7 mRNA 均有不同程度的表达,其中 AD 组 TRPC4 mRNA 表达较对照组明显增加(P < 0.01)。免疫荧光显示 TRPC4 表达在海马区的神经细胞膜上,且与对照组相比 AD 组 TRPC4 表达增加。Western blotting 结果显示 AD 组 TRPC4 蛋白表达较对照组增加(P < 0.05)。结论 侧脑室注射 Aβ1-42 寡聚体后,小鼠海马区 TRPC4 蛋白表达含量增加,提示 TRPC4 可能参与到钙稳态失调引起的 AD 的发病机 制中。

关键词: 经典瞬时受体电位通道, 阿尔茨海默病, β 淀粉样蛋白

Abstract: Objective To investigate the protein expression of the canonical transient receptor potential(TRPC)channel in the hippocampus of amyloid β protein(Aβ)-induced Alzheimer’s disease(AD)mice. Methods A total of 36 ICR mice were randomly divided into AD group and control group,with 18 rats in each group. AD mice models were established by Aβ 1 - 42 microinjection into the lateral intracerebroventricular. Learning and memory abilities of the mice were determined using Morris water maze. All TRPC1-TRPC7 mRNA levels in the hippocampus of the mice were detected using reverse transcriptase polymerase chain reaction(RT-PCR). Hippocampal TRPC4 protein expression was examined using immunofluorescence and Western blotting methods. Results Water maze test results showed that the escape latency of AD group were significantly longer than that of the control group(P < 0.01),and that the target quadrant occupancy of AD group was significantly shortened(P < 0.01)and the frequency of platform crossing of AD group was significantly reduced(P < 0.01). RT-PCR results showed that all TRPC(TRPC1-TRPC7) mRNA were expressed in the hippocampal of both AD group and control group. Among these channels,only TRPC4 mRNA levels of AD group was higher than that of the control group(P < 0.01). Immunofluorescence images showed that TRPC4 expressed on the membrane of neurons and the intensities of the immunofluorescence of TRPC4 in AD group were stronger than that of control group. Western blotting results showed that the TRPC4 protein expression of AD group was higher than that of control group(P < 0.05). Conclusion The increase of TRPC4 protein expression in the hippocampus of mice after intracerebroventricular injection of Aβ1-42 oligomers suggests that TRPC4 may be involved in the pathogenesis of AD induced by calcium homeostasis.

Key words: canonical transient receptor potential channel, Alzheimer’s disease, amyloid β protein

中图分类号: 

  • R363.1
[1] 王海英, 李慧源, 罗红月, 张慧予, 姜扬, 郭利晴, 陶蕾, 孙晓红. 青蒿素调控NF-κB信号通路介导阿尔茨海默病炎症反应细胞模型的研究[J]. 中国医科大学学报, 2018, 47(6): 552-555,561.
[2] 王思亓, 李欣潞, 林庚, 王卓, 程晓凤, 刘彤彤, 郑玮. DMT1在APP/PS1转基因小鼠小脑皮质中表达上调[J]. 中国医科大学学报, 2018, 47(3): 193-197.
[3] 张利华, 张丽艳, 张小康, 徐超龙, 周义. β-淀粉样蛋白诱导的阿尔茨海默病大鼠模型中BDNF与TRPC3的关系[J]. 中国医科大学学报, 2018, 47(3): 217-221.
[4] 丛琳,张楠楠,佡剑非,任艳. 甲基化芯片检测APP/PS1双转基因小鼠基因组DNA甲基化分布[J]. 中国医科大学学报, 2015, 44(2): 160-163.
[5] 费洪新,韩玉生,杜徽,姜波,李宝龙,朴成玉,张英博,仲丽丽,白云,周忠光. 补阳还五汤对APP/PS1双转基因小鼠学习记忆及海马组织白介素?6水平的影响[J]. 中国医科大学学报, 2014, 43(8): 677-681.
[6] 赵彬,商秀丽 ,何志义,范国光,刘虎. 阿尔茨海默病的静息态fMRI低频振幅研究[J]. 中国医科大学学报, 2012, 41(4): 329-332.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!

中国医科大学学报版权所有©2018

未经允许,严禁擅自转载本站图文资料

地址:中国 沈阳市沈北新区蒲河路77号 110122

辽ICP备05014850

JOURNAL OF CHINA MEDICAL UNIVERSITY

ADDRESS: NO.77 PUHE ROAD

SHENYANG NORTH NEW AREA, SHENYANG

LIAONING PROVINCE, P.R. CHINA