中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2017, Vol. 46 ›› Issue (1): 11-16.doi: 10.12007/j.issn.0258-4646.2017.01.003

• 论著 • 上一篇    下一篇

小鼠SPINK5 基因重组过表达腺病毒载体构建及其对特应性皮炎小鼠模型皮损疗效的研究

狄正鸿 1,许静 1,侯殿东 2,纪莲 3,刘晓梅 3,孙鲁宁 4   

  1. 中国医科大学 1附属盛京医院皮肤科,沈阳 110004;2附属第一医院皮肤科,沈阳 110001;3附属盛京医院实验中心,沈阳110004;4基础医学院病理生理学教研室,沈阳 110122
  • 收稿日期:2016-05-07 出版日期:2017-01-28 发布日期:2017-01-10
  • 作者简介:狄正鸿(1973 -),女,副教授,博士 .
  • 基金资助:
    国家自然科学基金(81301366);辽宁省自然科学基金(2013021004,2014021056)

Construction of Recombinant Adenovirus Vector Expressing Mouse SPINK5 Gene and Its Curative Effect on Skin Lesions in Atopic Dermatitis Mice

DI Zhenghong1,XU Jing1,HOU Diandong2,JI Lian3,LIU Xiaomei3,SUN Luning4   

  1. 1Department of Dermatology,Shengjing Hospital,China Medical University,Shenyang 110004,China;2 Department of Dermatology,The First Hospital,China Medical University,Shenyang 110001,China;3Experiment Center,Shengjing Hospital,China Medical University,Shenyang 110004,China;4Department of Pathophysiology,College of Basic Medical Science,China Medical University,Shenyang 110122,China
  • Received:2016-05-07 Online:2017-01-28 Published:2017-01-10

摘要: 目的 构建表达小鼠 SPINK5 基因的重组腺病毒载体,观察其对特应性皮炎小鼠模型的治疗效果。方法 采用 DNA 重 组技术,将携带小鼠 SPINK5 基因的腺病毒穿梭质粒与携带腺病毒基因组的包装质粒共转染 HEK293 细胞,产生重组腺病毒。 采用卵清蛋白,通过腹腔,皮下注射系统致敏结合皮肤局部外涂局部致敏 Balb/c 小鼠,建立特应性皮炎小鼠模型。将携带 SPINK5 基因的腺病毒载体注射至特应性皮炎小鼠模型皮损内,观测其对特应性皮炎小鼠模型的治疗效应。结果 成功构建了 表达小鼠 SPINK5 基因的重组腺病毒载体及小鼠特应性皮炎模型,该病毒载体注射于特应性皮炎小鼠模型皮损内,治疗 2 周可 明显减轻皮损处潮红,水肿及炎性反应。皮损处病理检测显示,与模型对照相比较,皮损厚度,炎性细胞浸润明显改善。结论 SPINK5 基因治疗对特应性皮炎小鼠模型具有明显的治疗效果。SPINK5 基因产物局部外用有望用于特应性皮炎的治疗。

关键词: SPINK5 基因, 重组腺病毒载体, 特应性皮炎, 小鼠模型

Abstract: Objective To construct a recombinant adenovirus vector expressing mouse SPINK5 gene,and observe its curative effect on the skin lesions in atopic dermatitis mice model. Methods By recombining DNA technology,the sequence of mouse SPINK5 gene was cloned into adenovirus shuttle plasmid. Then it was transformed into HEK 293 cells with the adenoviral backbone plasmid to obtain the recombinant adenovirus. A mouse model of atopic dermatitis was established by system and local sensitization of Balb/c mice with ovalbumin . The effect of recombinant adenovirus on the lesions of atopic dermatitis mice model was observed. Results The SPINK5 over-expressing adenovirus vector and atopic dermatitis mice model were successfully constructed. After 2 weeks of adenovirus-mediated SPINK5 gene intracutaneous injection,the redness and edema of lesions of AD model mice were obvious relieved. The pathological detection indicated that epidermal thickness and prickle cell layer ,inflammatory cell infiltration significant decreased accompanied with the model blank control. Conclusion The adenovirus-mediated SPINK5 gene had signifi- cant therapeutic effect to the atopic dermatitis mice model,which provided a laboratory basis of application of SPINK5 gene product to therapy atopic dermatitis.

Key words: SPINK5 gene, recombinant adenovirus vector, atopic dermatitis, mice model

中图分类号: 

  • R751.05
[1] 余惠珍,向红黄,舒洁,朱鹏立,潘玮,张枫. TK1和TIMP1基因共表达载体的构建及其在大鼠血管平滑肌细胞中的表达[J]. 中国医科大学学报, 2013, 42(12): 1072-1078.
[2] 姚立杰,金海峰,马勇,谢立平. 血管内皮生长因子D在肌肉瘤小鼠模型中的表达[J]. 中国医科大学学报, 2012, 41(1): 45-47.
[3] 张丽, 齐瑞群, 孙艳, 陈国红, 任宏伟, 陈洪铎, 高兴华. 卡介菌多糖核酸对特应性皮炎外周血CLA~+T细胞表达不同细胞因子的影响[J]. 中国医科大学学报, 2009, 38(10): 764-.
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