中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2017, Vol. 46 ›› Issue (3): 197-200.doi: 10.12007/j.issn.0258-4646.2017.03.002

• 论著 • 上一篇    下一篇

胆维丁乳对酵母多糖致多器官功能衰竭小鼠肝脏的作用

赵晴 1,娄岩 2,孙灿 1,孔娟 1   

  1. 中国医科大学 1 附属盛京医院临床营养科,沈阳 110004;2 公共基础学院计算机教研室,沈阳 110122
  • 收稿日期:2016-09-02 出版日期:2017-03-30 发布日期:2017-01-20
  • 通讯作者: 孔娟 E-mail:kongj1@sj?hospital.org
  • 作者简介:赵晴(1982 -),女,主治医师,硕士 .
  • 基金资助:
    国家自然科学基金(81170065,81570811)

Effect of Cholecalciterol Cholesterol Emulsion on Liver of Zymosan-induced Multiple Organ Failure in Mice

ZHAO Qing1,LOU Yan2,SUN Can1,KONG Juan1   

  1. 1 Department of Clinical Nutrition,Shengjing Hospital,China Medical University,Shenyang 110004,China;2 Department of Computer,School of Fundamental Sciences,China Medical University,Shenyang 110122,China
  • Received:2016-09-02 Online:2017-03-30 Published:2017-01-20

摘要: 目的 研究胆维丁乳对酵母多糖致多器官功能衰竭小鼠肝脏损伤的作用。方法 C57 BL/6 源性野生小鼠 36 只,随机分 为阴性对照(CON)组、胆维丁乳(CCE)组、酵母多糖(Z)组、CCE+Z 组,每组各 9 只。CON 组及 Z 组纯水喂养,CCE 组及 CCE+Z 组 应用胆维丁乳(20 μL 溶于 200 mL 纯水)避光喂养。14 d 后 Z 组及 CCE+Z 组酵母多糖(500 mg/kg)腹腔注射。18 h 后处死各组小 鼠,留肝脏标本行病理组织学检查;检测各组小鼠血清中谷丙转氨酶(ALT)含量;应用 Western blotting、实时 PCR 方法测定各组 小鼠肝脏组织白细胞介素 6(IL-6)、白细胞介素 18(IL-18)表达。结果 病理组织学显示,CCE+Z 组组织损伤较 Z 组轻,较对照 组重。与 CON 组比较,血清 ALT 水平 Z 组、CCE+Z 组升高;而 CCE 组降低,差异均具有统计学意义(均 P < 0. 05)。IL-6、IL-18 蛋 白与 mRNA 表达结果显示,与 CON 组及 CCE+Z 组比较 Z 组明显升高,差异具有统计学意义(均 P < 0. 05)。结论 胆维丁乳对酵 母多糖致肝脏损伤能够起到一定的保护作用。

关键词: 胆维丁乳, 酵母多糖, 细胞因子, 肝脏损伤

Abstract: Objective To study the effect of cholecalciterol cholesterol emulsion(CCE)in the zymosan(Z)- induced acute hepatic injury. Methods A total of 36 C57 BL/6 mice were randomly divided into 4 groups,namely negative contro(l CON)group,CCE group,Z group and CCE+Z group,respectively. There were 9 mice in each group. Mice from CON group and Z group were fed with pure water. Mice from CCE group and CCE+Z group were fed with cholecalciterol cholesterol emulsion 20 μL dissolved in 200 mL pure water which was kept in darkness. After 14 days, Z group and CCE+Z group were injected with zymosan at a dose of 500 mg/kg. After 18 hours,all the mice in each group were sacrificed. The liver tissues were harvested for histopathological examination. The serum ALT levels were determined. The molecular expression of IL-6 and IL-18 in liver tissue of mice were evaluated by Western blotting and real-time quantitative PCR method. Results The results of histopathological examination showed that liver tissue damage in CCE+Z group was lighter than that of Z group,and heavier than that of the CON group. Compared to the CON group,Z group had the highest serum ALT level,followed by CCE+Z group,while in Z group was significantly lower than that in CON group(all P < 0.05). The expression of IL-6 and IL-18 protein and mRNA showed level of Z group was apparently higher than those of CON group and CCE+Z group(all P < 0.05). Conclusion Cholecalciterol cholesterol emulsion can play certain protective effect on zymosan-induced liver injury in mice.

Key words: cholecalciterol cholesterol emulsion, zymosan, cytokine, hepatic injury

中图分类号: 

  • R332
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