中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2017, Vol. 46 ›› Issue (3): 232-237.doi: 10.12007/j.issn.0258-4646.2017.03.010

• 论著 • 上一篇    下一篇

高脂饮食对 AOM/DSS 诱导的小鼠不同周期溃疡性结肠炎的 影响及白细胞介素 6 的改变

周凤 1,2,刘维新 1,于艳红 1,邢俊伟 1,杨慧珊 1,李虹 1,兰雨桐 1   

  1. 1 中国医科大学附属第一医院消化内科,沈阳 110001;2 宁波市医疗中心李惠利东部医院感染科,浙江 宁波 315000
  • 收稿日期:2016-07-05 出版日期:2017-03-30 发布日期:2017-01-20
  • 通讯作者: 刘维新 E-mail:weixinliu@yahoo.com
  • 作者简介:周凤(1991 -),女,医师,硕士 .
  • 基金资助:
    沈阳市科技计划(F13?316?1?40)

Effects of High Fat Diet on Ulcerative Colitis in Different Periods Induced by AOM/DSS and the Changes of Interleukin-6 Level in Mice

ZHOU Feng1,2,LIU Weixin1,YU Yanhong1,XING Junwei1,YANG Huishan1,LI Hong1,LAN Yutong1   

  1. 1 Department of Digestive Diseases,The First Hospital,China Medical University,Shenyang 110001,China;2 Department of Infectious Diseases,Ningbo Medical Center Lihuili Eastern Hospital,Ningbo 315000,China
  • Received:2016-07-05 Online:2017-03-30 Published:2017-01-20

摘要: 目的 观察高脂饮食对氧化偶氮甲烷(AOM)/葡聚糖硫酸钠(DSS)诱导的小鼠不同周期溃疡性结肠炎(UC)及其不典型增生的影响以及血液中白细胞介素 6(IL-6)的改变。方法 DSS 组、DSS+AOM 组、DSS+高脂组、DSS+AOM+高脂组小鼠以 DSS 水 3 d+灭菌水 4 d 为 1 个循环周期全程交替口服,并设立正常对照组,每组 12 只。前 3 周每周第 1 天 DSS+AOM 组及 DSS+AOM+ 高脂组小鼠腹腔注射 AOM(10 mg/kg)。分段处死小鼠,采用疾病活动指数、病理组织学评分判断炎症程度,ELISA 法检测血清中 IL-6 水平。结果 单纯使用 DSS 可建立 UC 模型。在第 9 个循环周期末处死的小鼠中,正常对照组与 DSS 组无不典型增生发生,DSS+高脂组不典型增生率为 25%(1/4),而 DSS+AOM 组为 50%(2/4),DSS+AOM+高脂组为 75%(3/4),但 DSS 组与 DSS+高脂组、DSS+AOM 组与 DSS+AOM+高脂组、DSS 组与 DSS+AOM 组、DSS+高脂组与 DSS+AOM+高脂组比较,不典型增生率均无统计学差异(均 P >0.05)。DSS+高脂组和 DSS+AOM+高脂组的疾病活动指数、病理组织学评分分别大于对应的 DSS 组和 DSS+AOM 组(P < 0.05)。DSS+高脂组和 DSS+AOM+高脂组血清 IL-6 水平虽高于对应的 DSS 组和 DSS+AOM 组,但差异无统计学意义(P >0.05)。结论 高脂饮食可能是 UC 及其不典型增生的一种致病因素。

关键词: 高脂饮食, 溃疡性结肠炎, 不典型增生, 白细胞介素 6

Abstract: Objective To observe the effects of high fat diet on ulcerative colitis(UC)and atypical hyperplasia in different periods induced by azoxymethane(AOM)/dextran sodium sulfate(DSS)and the changes of interleukin-6(IL-6)level in blood. Methods The mice in DSS,DSS+ AOM,DSS+high fat diet,and DSS+AOM+high fat diet groups were given DSS for 3 days and sterilization water for 4 days as one cycle for 9 cycles, and the mice in normal control group were given sterilization water(n = 12 in each group). The mice in DSS+AOM and DSS+AOM+high fat diet groups received intraperitoneal injection of AOM(10 mg/kg)in the every first day of the first 3 cycles. The mice in each group were sacrificed at different time points,and the disease activity index and pathohistological index were used to determine the degree of inflammation. ELISA method was used for the detection of serum IL-6 level. Results Simple administration of DSS could induce UC in the mouse model. After 9 circles of treatment,atypical hyperplasia was not found in normal control and DSS groups,and the rate of atypical hyperplasia was 25%(1/4)in DSS+high fat diet group,50%(2/4)in DSS+AOM group,and 75%(3/4)in DSS+AOM+high fat diet group. However,there were no significant differences in the rate of atypical hyperplasia between DSS and DSS+AOM groups,DSS+high fat diet and DSS+AOM+high fat diet groups,DSS and DSS+high fat diet groups,and DSS+AOM and DSS+AOM+high fat diet groups(all P > 0.05). The histopathological score and the disease activity index in DSS+high fat diet and DSS+AOM+high fat diet groups were higher than those in DSS and DSS+AOM groups(P < 0.05). The IL-6 level in DSS+ high fat diet and DSS+AOM+high fat diet groups was higher than that in DSS and DSS+AOM groups,but the difference was not statistically signifi- cant(P > 0.05). Conclusion High fat diet may be one of the stimulating factors of UC and atypical hyperplasia.

Key words: high fat diet, ulcerative colitis, atypical hyperplasia, interleukin-6

中图分类号: 

  • R574.62
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