中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2017, Vol. 46 ›› Issue (6): 501-504.doi: 10.12007/j.issn.0258-4646.2017.06.005

• 论著 • 上一篇    下一篇

载脂蛋白 AI 对单核细胞表型与 MCP-1/CCR2 的影响

尹建国 1,2,张社兵 1,彭道泉 2   

  1. 1 汕头大学医学院附属粤北人民医院心血管内科,广东 韶关 512026;2 中南大学湘雅二医院心血管内科,长沙 410011
  • 收稿日期:2016-10-12 出版日期:2017-06-30 发布日期:2017-06-07
  • 通讯作者: 彭道泉 E-mail:pengdq@hotmail.com
  • 作者简介:尹建国(1978 -),男,副主任医师,博士 .
  • 基金资助:
    国家自然科学基金(81370393)

Effect of Apolipoprotein AI on MCP-1 Plasma Levels,Monocyte Subset Proportions,and in Vitro CCR2 Expression

YIN Jianguo1,2,ZHANG Shebing1,PENG Daoquan2   

  1. 1 Department of Cardiology,Yuebei People’s Hospital Affiliated to Shantou University Medical College,Shaoguan 512026,China;2 Department of Cardiology,The Second Xiangya Hospital of Central South University,Changsha 410011,China
  • Received:2016-10-12 Online:2017-06-30 Published:2017-06-07

摘要: 目的 探讨载脂蛋白 AI(apoAI)对动脉粥样硬化小鼠血液、脾脏中 Ly6Chi 单核细胞和单核细胞趋化蛋白 1(MCP-1)浓度 及 CC 类趋化因子受体 2(CCR2)表达的影响。方法 16 只雄性 apoE-/-小鼠高脂饲料饲养 12 周后随机分为对照组和 apoAI 组,处死前第 1、3 天分别予以生理盐水和 apoAI(40 mg/kg)干预,处死 12 h 前予以腹腔注射脂多糖(LPS)25 μg/只,酶联免疫法检测血 浆 MCP-1 浓度,流式细胞仪检测血液、脾脏中 Ly6Chi 单核细胞比例。THP-1 细胞随机分为对照组和 apoAI 组,予以相应干预后加入浓度为 10 μg/L 的 LPS 孵育,Western blotting 检测 2 组中 CCR2 的表达。结果 与对照组比较,apoAI 能显著降低血液及脾脏中 Ly6Chi 单核细胞比例及血浆中 MCP-1 浓度(P < 0.01),体外研究显示 apoAI 能下调单核细胞 CCR2 的表达(P < 0.05)。 结论 apoAI 能降低 MCP-1 浓度及血液、脾脏中 Ly6Chi 单核细胞比例,抑制 CCR2 的表达。

关键词: 载脂蛋白 AI, 单核细胞表型, 单核细胞趋化蛋白 1, CC 类趋化因子受体 2

Abstract: Objective To explore effects of apoAI on MCP-1 levels in the plasma and the Ly6Chi monocyte proportion in the blood and spleen of atherosclerotic mice,as well as on CCR2 expression in vitro. Methods Sixteen male apoE-/- mice were fed with high cholesterol diet for 12 weeks. Mice were randomly divided into control and apoAI groups and were administered with PBS or apoAI(40 mg/kg),respectively,via tail vein on the 1st and 3rd day before sacrifice. Mice in both groups were administered LPS(25 μg/mouse)via intraperitoneal injection 12 h before sacrifice. Plasma levels of MCP- 1 were determined using ELISA,and the Ly6Chi monocyte proportion was analyzed using flow cytometry. In addition,human monocytic THP-1 cells were randomly divided into control and apoAI(10 mg/L)-treated groups,pretreated with corresponding intervention,and incubated with LPS(10 μg/L). CCR2 expression levels were measured by Western blotting. Results Compared with the control treatment, apoAI treatment remarkably reduced MCP-1 levels in plasma and Ly6Chi monocyte proportion in the blood and spleen(P < 0.01). Furthermore, apoAI treatment inhibited CCR2 expression in monocytes in vitro(P < 0.05). Conclusion apoAI can reduce MCP-1 levels in plasma and the Ly6Chi monocyte proportion in the blood and spleen and can inhibit CCR2 expression in monocytes in vitro.

Key words: apolipoprotein AI, monocyte subset, monocyte chemotactic protein 1, CC-chemokine receptor 2

中图分类号: 

  • R543.3
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