中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2017, Vol. 46 ›› Issue (6): 510-514.doi: 10.12007/j.issn.0258-4646.2017.06.007

• 论著 • 上一篇    下一篇

应用 iTRAQ 技术筛选先天性心脏畸形胎儿母亲血清差异表达蛋白

陈骊珠 1,马巍 2,张墨 3,袁正伟 2   

  1. 中国医科大学附属盛京医院 1 超声科;2 国家卫生和计划生育委员会小儿先天畸形重点实验室;3 泌尿外科,沈阳 110004
  • 收稿日期:2016-10-17 出版日期:2017-06-30 发布日期:2017-06-07
  • 通讯作者: 袁正伟 E-mail:yuanzw@hotmail.com
  • 作者简介:陈骊珠(1984 -),女,讲师,博士 .
  • 基金资助:
    国家自然科学基金青年基金项目(81600258);国家重点研发计划(2016YFC1000505);国家自然科学基金(81671469)

The Identification of Differentially Expressed Proteins in Maternal Serum of Fetuses with Congenital Heart Defects by Using the iTRAQ Technology

CHEN Lizhu1,MA Wei2,ZHANG Mo3,YUAN Zhengwei2   

  1. 1 Department of Ultrasound,Shengjing Hospital,China Medical University,Shenyang 110004,China;2 Key Laboratory of Health Ministry for Congenital Malformation,Shengjing Hospital,China Medical University,Shenyang 110004,China;3 Department of Urology,Shengjing Hospital,China Medical University,Shenyang 110004,China
  • Received:2016-10-17 Online:2017-06-30 Published:2017-06-07

摘要: 目的 筛选先天性心脏畸形(CHD)母体血清差异表达蛋白质,明确和 CHD 相关的候选标志物。方法 收集 40 例 CHD 胎儿(实验组)母亲血清和 10 例正常胎儿(对照组)母亲血清,其中实验组包括法洛氏四联征 10 例,室间隔缺损 10 例,共同动脉 干 10 例,其他少见先天性心脏畸形 10 例。每组 10 例标本等体积混合后,应用同位素标记相对和绝对定量技术(iTRAQ)对蛋白 质进行鉴定和相对定量。结果 孕妇外周血血清中共鉴定到蛋白质 606 个,实验组与对照组差异达到 1.5 倍以上的蛋白质 47 个,其中 23 个在实验组中上调,24 个在实验组中下调。 结论 基于 iTRAQ 技术的蛋白质组学方法能鉴定出多种 CHD 相关的差 异蛋白,CHD 发生是一个多种蛋白质分子参与的结果。

关键词: 同位素标记相对和绝对定量技术, 先天性心脏畸形, 蛋白质组学, 血清标志物, 产前诊断

Abstract: Objective To screen for serum protein differentially expressed between women whose fetuses had congenital heart defects(CHD) and women who had normal fetuses. Methods Serum samples were collected from pregnant women whose fetuses had CHD and those whose fetuses had no CHD,including a CHD group of 40 women and a control group of 10 women. The CHD group included 4 subgroups as follows:tetralogy of Fallot,ventricular septal defects,persistent truncus arteriosus,and a mixture of relatively rare types of CHD(n = 10 each). Samples in the same group were pooled to obtain equal amounts of proteins,and the iTRAQ proteomic approach was used to identify and quantify the proteins differentially expressed among these groups. Results We successfully identified 606 proteins,among which 47 showed at least a 1.5-fold difference between the CHD and control groups. Among the 47 proteins,23 and 24 were upregulated and downregulated,respectively. Conclusion Several proteins associated with CHD could be identified by using the iTRAQ proteomic approach,and various proteins were involved in the pathogenesis of CHD in this study.

Key words: isobaric tag for relative and absolute quantitation, congenital heart defect, proteomics, serum biomarker, prenatal diagnosis

中图分类号: 

  • R541.1
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