中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2017, Vol. 46 ›› Issue (6): 532-535.doi: 10.12007/j.issn.0258-4646.2017.06.012

• 论著 • 上一篇    下一篇

膜联蛋白 A1 对糖尿病大鼠心功能及炎症反应的影响

陈建康 1,严瑜 1,潘晓莉 1,张泓 1,林帆 1,朱鹏立 1,柯俊 2   

  1. 福建省立医院,福建省医科大学省立临床医学院 1 老年科,福建省临床老年病研究所;2 急诊内科,福建省急诊医学研究所,福州350001
  • 收稿日期:2016-12-13 出版日期:2017-06-30 发布日期:2017-06-07
  • 通讯作者: 柯俊 E-mail:68223384@qq.com
  • 作者简介:陈建康(1975 -),男,副主任医师,硕士 .
  • 基金资助:
    福建省自然科学基金(2016J01431)

Effect of Annexin A1 on the Cardiac Function and Inflammatory Response of Diabetic Rats

CHEN Jiankang1,YAN Yu1,PAN Xiaoli1,ZHANG Hong1,LIN Fan1,ZHU Pengli1,KE Jun2   

  1. 1 Department of Geriatric Medicine,Fujian Institute of Clinical Geriatrics,Fujian Provincial Hospital,Provincial Clinical College,Fujian Medical University,Fuzhou 350001,China;2 Department of Emergency,Fujian Provincial Institute of Emergency Medicine,Fujian Provincial Hospital,Provincial Clinical College,Fujian Medical University,Fuzhou 350001,China
  • Received:2016-12-13 Online:2017-06-30 Published:2017-06-07

摘要: 目的 探讨膜联蛋白 A1(Annexin A1)对糖尿病大鼠心功能及肿瘤坏死因子 α(TNF-α)、白细胞介素 1β(IL-1β)的影响 。 方法 将 24 只 SD 大鼠随机均分为正常对照组、糖尿病组;利用高糖高脂饮食饲养及较小剂量链脲佐菌素(30 mg/kg)诱导造成 2 型糖尿病模型,2 组分别予以普通喂食、高糖高脂喂食;于第 8 周末用彩色多普勒超声检测左心室舒张末期容积(LVEDV)、左心室收缩末期容积(LVESV)、二尖瓣舒张早期峰值速度/二尖瓣舒张晚期峰值速度(e/a)比值、左心室射血分数(LVEF)、每搏射血量(SV);第 0、4、8 周采用实时 PCR 测定血中 Annexin A1 表达,采用酶联免疫分析法测定血中 TNF-α、IL-1β。结果 与正常对照组对比,糖尿病组 LVEDV、e/a、SV 明显下降,Annexin A1 明显上升(P < 0.05),8 周后糖尿病组 Annexin A1 显著下降(P < 0.01);与 正常对照组对比,糖尿病组 TNF-α、IL-1β 上升明显(P < 0.05),8 周后上升更明显(P<0.01);糖尿病组 Annexin A1 与 TNF-α、IL-1β 负相关(r 分别为-0.73、-0.71,P < 0.01)。结论 Annexin A1 可改善糖尿病大鼠心功能,Annexin A1 改善糖尿病大鼠心功能可能与炎症反应有关。

关键词: 膜联蛋白 A1, 心功能, 2 型糖尿病, 炎症反应

Abstract: Objective To study the effect of annexin A1 on cardiac function,tumor necrosis factor α(TNF-α),and interleukin 1β(IL-1β)in diabetic rats. Methods Twenty-four SD rats were randomly divided into normal control and diabetic groups. The type 2 diabetes model was induced with a high-glucose and high-fat diet and administration of low-dose streptozotocin.Left ventricular end-diastolic volume(LVEDV),left ventricular end-systolic volume(LVESV),peak velocity of early diastolic mitral-to-late diastolic peak velocity(e/a)ratio,left ventricular ejection fraction(LVEF),and stroke volume(SV)were measured by using color Doppler ultrasonography at the end of week 8. The expression levels of TNF-α and IL-1β in blood were measured by using enzyme-linked immunosorbent assay,and the expression level of annexin A1 in blood was measured at weeks 0,4,and 8 by using real-time polymerase chain reaction. Results Compared with the normal control group,the diabetic group had de- creased LVEDV,e/a,and SV(P < 0.05).The annexin A1 expression level in the diabetic group decreased significantly after 8 weeks(P < 0.01). The TNF-α and IL-1β levels in the diabetic group were significantly higher than those in the normal control group(P < 0.05)and increased signifi- cantly after 8 weeks(P < 0.01). Annexin A1 level correlated with the TNF- α and IL-1β levels in the diabetic group(P < 0.01). Conclusion Annexin A1 expression shows an anti-inflammatory effect that improved the cardiac function of diabetic rats.

Key words: Annexin A1, cardiac function, type 2 diabetes, inflammation

中图分类号: 

  • R544.1
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