中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2017, Vol. 46 ›› Issue (7): 582-586.doi: 10.12007/j.issn.0258-4646.2017.07.002

• 论著 • 上一篇    下一篇

纯化兔血清对氧磷酶 1 与传统方法对敌敌畏所致大鼠 肝脏损伤的疗效比较

王聪,陶维晨,李琦,吴泽扬,郭镇豪,赵敏   

  1. 中国医科大学附属盛京医院急诊科,沈阳 110004
  • 收稿日期:2016-10-25 出版日期:2017-07-31 发布日期:2017-07-21
  • 通讯作者: 赵敏 E-mail:zhaom@sj?hospital.org
  • 作者简介:王聪(1992 -),女,医师,硕士研究生 .
  • 基金资助:
    国家自然科学基金(81671898);盛京自由研究者计划(201207)

Use of Purified Rabbit Serum Paraoxonase 1 Compared with the Traditional Method of Curative Effect on Dichlorvos-induced Liver Injury in Rats

WANG Cong,TAO Weichen,LI Qi,WU Zeyang,GUO Zhenhao,ZHAO Min   

  1. Emergency Department,Shengjing Hospital,China Medical University,Shenyang 110004,China
  • Received:2016-10-25 Online:2017-07-31 Published:2017-07-21

摘要: 目的 探讨纯化兔血清对氧磷酶 1(PON1)对敌敌畏中毒大鼠肝损伤的疗效是否优于传统方法。 方法 将 30 只雄性 SD大鼠随机分为 5 组:空白对照组(A 组)、敌敌畏染毒组(B 组)、传统治疗组(C 组)、PON1 治疗组(D 组)、联合治疗组(E 组),每组 6只 。 B、C、D、E 组均给予敌敌畏(9 mg/kg)腹腔注射染毒 ,C 组在染毒后立即(<2 min)给 予 阿 托 品(10 mg/kg)+碘 解 磷 定(45mg/kg)腹腔注射,D 组在染毒前 30 min 给予 PON1 9 600 U/kg 尾静脉注射,E 组染毒前 30 min 给予 PON1 9 600 U/kg 尾静脉注射,染毒后立即(<2 min)给予阿托品(10 mg/kg)+碘解磷定(45 mg/kg)腹腔注射,A 组给予等量的生理盐水对照。各组于造模前(0min)、造模后 10 min、30 min、1 h、2 h、4 h、6 h 分别颈内动脉取血,运用 ELISA 方法检测血清乙酰胆碱酯酶活性。各组分别于造模后 12 h 取肝脏组织,HE 染色观察不同组肝脏的病理改变,运用免疫组化及 Western blotting 检测不同组肝脏 4-羟基壬烯醛(4-HNE)的定性及定量表达。结果 B 组胆碱酯酶明显下降,光镜下出现肝细胞严重脂肪变、核固缩等病理改变,可见 4-HNE高度表达;C、D、E 组上述各指标改变均较 B 组轻,且各组与 B 组之间均存在统计学差异(P < 0.05);D、E 组病理改变较 C 组轻,上述指标与 C 组之间均有统计学差异(P < 0.05);E 组较 D 组病理改变稍轻,上述指标无统计学差异(P > 0.05)。结论 PON1 对急性敌敌畏中毒大鼠肝损伤发挥保护作用,且效果优于传统治疗。

关键词: 对氧磷酶 1, 敌敌畏中毒, 肝损伤, 氧化应激, 4-羟基壬烯

Abstract: Objective To explore whether the use of purified rabbit serum paraoxonase 1(PON1)for the treatment of dichlorvos-induced liver injury in rats is superior to traditional method. Methodse Thirty male SD rats were randomly divided into the followint 5 groups,with 6 rats in each group:control group(A group),dichlorvos group(B group),traditional treatment group(C group),PON1 treatment group(D group),combined treatment group(E group). Rats in groups B,C,D and E were adminstered dichlorvos by intraperitoneal injection 9 mg/kg. In group C,atropine 10 mg/kg and iodine solution 45 mg/kg were injected intraperitoneally within 2 min after dichlorvos administration. In group D,PON1 was injected intravenously at a dose of 9 600 U/kg,30 min prior to poisoning. In group E,PON1 was injected intravenously at a dose of 9 600 U/kg,30 min prior to poisoning,followed by in travenous injection of atropine 10 mg/kg and iodine solution 45 mg/kg within 2 min after poisoning. Rats in A group received normal saline. Blood was collected at different time points to examine the acetyl cholinesterase(AChE)-levels by ELISA method. Liver tissue were collected at 12 hours after model establishment to observe the pathological changes. The expression of 4 hydroxy 2-nonenal(4-HNE)in the liver tissue was detected by immunohistochemistry and Western blotting. Results In group B,AChE levels decreased significantly,liver cells showed severe fatty degeneration,karyopyknosis and other pathological changes,and 4-HNE expression increased. The pathological changes of group D and group E were less obvious than those of group C,and the 4-HNE expression in the group D and group E were significantly different from that in the group C(P < 0.05). Conclusion PON1 plays a protective role in dichlorvos-induced liver injury in rats, and this protection is better than that offered by traditional treatment.

Key words: paraoxonase 1, dichlorvos poisoning, liver injury, oxidative stress, 4-hydroxy 2-nonenal

中图分类号: 

  • R575.5
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