中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2017, Vol. 46 ›› Issue (7): 640-644.doi: 10.12007/j.issn.0258-4646.2017.07.015

• 论著 • 上一篇    下一篇

乌梅提取物对纳米细菌致大鼠肾损伤的修复与拮抗结石生成的 关系研究

夏剑锋,谷江,张永春,刘淼,董安涛,杨锦春   

  1. 贵州医科大学附属医院泌尿外科,贵阳 550004
  • 收稿日期:2016-11-09 出版日期:2017-07-31 发布日期:2017-07-21
  • 通讯作者: 谷江 E-mail:gj0851@Gmail.com
  • 作者简介:夏剑锋(1989 -),男,硕士研究生 .
  • 基金资助:
    贵阳市科技计划(2014100145)

Effect of Fructus Mume Extract on Repair of Renal Injury Induced by Nanobacteria in Rats and Its Relationship with Antagonistic Stone Formation

XIA Jianfeng,GU Jiang,ZHANG Yongchun,LIU Miao,DONG Antao,YANG Jinchun   

  1. Department of Urology,The Hospital Affiliated of Guizhou Medcial University,Guiyang 550004,China
  • Received:2016-11-09 Online:2017-07-31 Published:2017-07-21

摘要: 目的 构建纳米细菌(NB)致肾结石大鼠模型,观察乌梅提取物对早期肾损伤分子 1(KIM-1)和骨桥蛋白(OPN)的影响,分析乌梅提取物对早期肾结石的治疗意义。方法 从人上尿路结石分离并培养 NB。将 54 只雄性 SD 大鼠随机分为空白组、诱石组和治疗组,采取尾静脉注射 NB 构建肾结石模型,以乌梅提取物干预模型,并在不同时期分批处死大鼠,实时 PCR 检测肾组织 KIM-1 mRNA 的表达 ,ELISA 检测尿 KIM-1 浓度 ,HE 染色观察肾组织结石晶体情 况 ,并用免疫组化方法检测 OPN 表达 。结果 诱石组及治疗组肾结石模型大鼠出现肾小管扩张,肾小管结石晶体形成,早期 KIM-1 和 OPN 的表达升高,上述变化与 NB 的注射时间呈正相关,乌梅提取物可拮抗该类变化。结论 乌梅提取物可能通过修复肾损伤减少肾结石形成,该机制可能与乌梅提取物对 KIM-1、OPN 的基因调节有关。

关键词: 乌梅提取物, 纳米细菌, 肾结石, 损伤修复

Abstract: Objective To observe the early effect of Fructus mume extract on KIM-1 and OPN levels in rats with kidney stone formation induced by nanobacteria and to investigate the therapeutic significance of F. mume extract on early kidney stone formation. Methodse Nanobacteria were separated and cultured from human upper urinary calculi. The study group appropriately included 54 healthy male SD rats. The renal calculus model was constructed by tail vein injection of nanobacteria. A kidney stone model was created with an F. mume extract intervention,and rats were killed at different stages of the intervention. Real-time polymerase chain reaction was used to detect the KIM-1 mRNA expression in rat renal tissue, and the KIM-1 concentration in urine was detected by using enzyme-linked immunosorbent assay. We detected kidney tissue stone crystals and OPN expression by using hematoxylin-eosin staining and immunohistochemistry. Results Renal tubules of the experimental model were significantly expanded,that is,the formation of renal tubular stones. The early KIM-1 and OPN expression levels were increased. The above-mentioned changes positively correlated with the injection time of the nanobacteria,and F. mume extract antagonized the changes. Conclusion F. mume extract may be useful for the repair of renal injury to reduce kidney stone formation,which may be related to the gene regulation of KIM-1 and OPN.

Key words: fuctus mume extract, nanobacteria, kidney stones, injury repair

中图分类号: 

  • R692.4
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