中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2017, Vol. 46 ›› Issue (7): 645-648.doi: 10.12007/j.issn.0258-4646.2017.07.016

• 论著 • 上一篇    下一篇

粪肠球菌和酿酒酵母混合益生菌发酵条件的优化

孙冶 12,肖纯凌 2,海晓欧 1,李舒音 1,张迎今 1,李新鸣 12   

  1. 1 沈阳医学院基础医学院病原生物学教研室,沈阳 110034;1 辽宁省环境污染与微生态重点实验室,沈阳 110034
  • 收稿日期:2017-01-13 出版日期:2017-07-31 发布日期:2017-07-21
  • 通讯作者: 李新鸣 E-mail:2290354181@qq.com
  • 作者简介:孙冶(1987 -),男,助理实验员,硕士 .
  • 基金资助:
    沈阳市科技局科技项目(F15?199?1?29);沈阳市科技局2014 年市公益类科技项目

Optimization of Fermentation Conditions for Enterococcus faecalis and Saccharomyces cerevisiae Mixed Probiotic Formulation

SUN Ye12,XIAO Chunling2,HAI Xiaoou1,LI Shuyin1,ZHANG Yingjin1,LI Xinming12   

  1. 1 Department of Pathogenic Biology,Institute of Basic Medicine,Shenyang Medical College,Shenyang 110034,China;1 Liaoning Province Key Lab of Environment Pollution and Microecology,Shenyang 110034,China
  • Received:2017-01-13 Online:2017-07-31 Published:2017-07-21

摘要: 目的 研究粪肠球菌与酿酒酵母混合培养发酵条件的优化。方法 对粪肠球菌与酿酒酵母混合培养发酵条件进行优化,分析培养液初始 pH、接种量及通气量对混合菌生长的影响。结果 混合菌培养液最佳初始 pH 7.0,在 600 mL 中试发酵罐(装液量 40%)发酵温度 30 ℃、通气量 0.2 L/min、粪肠球菌和酿酒酵母接种量分别为 2.0%和 5.0%,发酵 20 h,发酵结束时粪肠球菌和酿酒酵母总菌数约为 3.8×108 CFU/mL 和 2.4×108 CFU/mL。通气量对于粪链球菌菌量差异无统计学意义(P > 0.05),而对于酿酒酵母菌量差异具有统计学意义(P < 0.05),结论 优化发酵条件可获得较高水平的混合益生菌菌量,优化后粪肠球菌和酿酒酵母菌量分别提高了 32.2%和 31.5%,可为混合益生菌制剂大规模生产提供参考。

关键词: 益生菌, 粪肠球菌, 酿酒酵母, 发酵培养

Abstract: Objective To optimize the fermentation conditions for Enterococcus faecalis(E.faecalis)and Saccharomyces cerevisiae(S.cerevisiae)mixed culture. Methodse The optimum fermentation conditions for E. faecalis and S. cerevisiae mixed culture were identified by investigating the influence of initial pH,inoculum size,and ventilation rate of the culture broth on mixed microbial growth. Results The optimal initial pH of mixed microbial culture was 7.0 and the inoculation amounts of E. faecalis and S. cerevisiae were 4% and 10% respectively,when fermented in 600-mL pilot fermentor(liquid volume 40%)at 30 ℃,with ventilation rate of 0.2 L/min,for 20 h. At the end of the fermentation,the E. faecalis and S. cerevisiae counts were approximately 3.8 × 108 CFU/mL and 2.4 × 108 CFU/mL,respectively. Ventilation for the amount difference of E. faecalis was no significan(t P > 0.05),and for the amount difference of S. cerevisiae was statistically significant(P < 0.05). Conclusion The E. faecalis and S. cerevisiae counts increased by 32.2% and 31.5% respectively,when the optimized conditions of fermentation culture were used. In this study,high mixed microbial counts were obtained,thus providing a reference for the preparation of large-scale production of mixed microbes.

Key words: probiotic, Enterococcus faecalis, Saccharomyces cerevisiae, fermentation probiotic

中图分类号: 

  • Q93
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