中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2017, Vol. 46 ›› Issue (12): 1071-1075,1081.doi: 10.12007/j.issn.0258-4646.2017.12.004

• 论著 • 上一篇    下一篇

金属蛋白酶7及金属蛋白酶19在胆道闭锁幼鼠动物模型肝脏组织中的表达

刘亭睁, 张志波   

  1. 中国医科大学附属盛京医院小儿外科, 沈阳 110004
  • 收稿日期:2017-04-10 出版日期:2017-12-30 发布日期:2017-12-08
  • 通讯作者: 张志波 E-mail:zhangzb@sj-hospital.org
  • 作者简介:刘亭睁(1989-),女,医师,硕士研究生.
  • 基金资助:
    国家自然科学基金(81270437)

Expression of Matrix Metalloproteinase-7 and -19 in Liver Tissues of Rats with Biliary Atresia

LIU Tingzheng, ZHANG Zhibo   

  1. Department of Pediatric Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China
  • Received:2017-04-10 Online:2017-12-30 Published:2017-12-08

摘要: 目的 检测金属蛋白酶家族中金属蛋白酶7(MMP-7)和金属蛋白酶19(MMP-19)在幼鼠胆道闭锁动物模型中的表达情况,探讨其在胆道闭锁肝纤维化中的作用及机制。方法 选取出生3 d的Wistar幼鼠132只作为实验组,采用肝外胆管结扎的方法制作胆道闭锁动物模型,62只幼鼠作为对照组,仅行开腹探查术。于术后3 d、7 d、14 d、21 d、28 d取肝脏组织,应用免疫组化、Western blotting及实时PCR等方法分别检测MMP-7及MMP-19在2组肝脏组织中的分布和表达情况。结果 实验组幼鼠在术后24 h即表现出梗阻性黄疸的临床症状;HE染色及Masson染色均发现肝脏组织纤维化逐渐加重;免疫组化、Western blotting、实时PCR等均发现实验组MMP-7的表达明显高于对照组,且逐渐升高;MMP-19在各时间段表达逐渐增强,但均低于对照组。结论 MMP-7和MMP-19在胆道闭锁肝纤维化中可能发挥重要作用。

关键词: 金属蛋白酶7, 金属蛋白酶19, 胆道闭锁, 肝纤维化

Abstract: Objective To detect the expression of matrix metalloproteinase (MMP)-7 and MMP-19 in the liver tissue of young rat models with biliary atresia (BA) and to explore their roles in progressive fibrosis. Methods Totally 132 Wistar newborn rats were subjected to common bile duct ligation (BDL) at day 3 after birth to establish animal models of biliary atresia. Sixty-two young rats served as the control group. Liver tissues were collected at days 3,7,14,21,and 28. Immunohistochemistry,Western blotting,and RT-PCR were used to detect the expression of MMP-7 and MMP-19. Results The BA models manifested cholestasis as early as 24 h after BDL. HE and Masson staining revealed progressive hepatic fibrosis. The expression of MMP-7 was observed to increase with time and was significantly higher in the experimental group when compared to control. The expression of MMP-19 was also found to gradually increase with time;however,it was lower than the control group. Conclusion MMP-7 and MMP-19 may be involved in the fibrosis of biliary atresia.

Key words: matrix metalloproteinase-7, matrix metalloproteinase-19, biliary atresia, hepatic fibrosis

中图分类号: 

  • R765.2
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