中国医科大学学报

中国医科大学学报
  • 中文核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
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中国医科大学学报 ›› 2017, Vol. 46 ›› Issue (12): 1082-1086.doi: 10.12007/j.issn.0258-4646.2017.12.006

• 论著 • 上一篇    下一篇

乙肝病毒X蛋白结合蛋白对腺样囊性癌细胞株ACC-2生物学行为的影响

孟雪1, 齐晓宇2, 刘维贤1, 王瑶3, 王秋旭1   

  1. 1. 中国医科大学附属盛京医院口腔颌面外科, 沈阳 110004;
    2. 辽宁省铁法煤业集团总医院口腔科, 辽宁 铁岭 112000;
    3. 沈阳农业大学畜牧兽医学院预防兽医学教研室, 沈阳 110866
  • 收稿日期:2017-03-31 出版日期:2017-12-30 发布日期:2017-12-08
  • 通讯作者: 王秋旭 E-mail:wangqx@sj-hospital.org
  • 作者简介:孟雪(1986-),女,医师,硕士.
  • 基金资助:
    辽宁省科学技术计划(2011010215-404)

Effect of HBXIP Expression on the Biological Behavior of the Adenoid Cystic Carcinoma Cell Line ACC-2

MENG Xue1, QI Xiaoyu2, LIU Weixian1, WANG Yao3, WANG Qiuxu1   

  1. 1. Department of Oral and Maxillofacial Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China;
    2. Department of Stomatology, Liaoning Province Tiefa Coal Group General Hospital, Tieling 112000, China;
    3. Teaching & Research Section of Preventive Veterinary Medicine, College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China
  • Received:2017-03-31 Online:2017-12-30 Published:2017-12-08

摘要: 目的 探讨乙肝病毒X蛋白结合蛋白(HBXIP)对腺样囊性癌细胞株ACC-2生物学行为的影响及其机制。方法 采用siRNA转染法抑制腺样囊性癌细胞株ACC-2中HBXIP的表达;实时PCR和Western blotting检测HBXIP基因及蛋白表达水平;MTT法检测HBXIP-siRNA对细胞增殖的影响;transwell法检测HBXIP-siRNA对细胞侵袭的影响;划痕实验检测HBXIP-siRNA对细胞迁移的影响;蛋白印迹方法检测HBXIP-siRNA对PI3K/Akt信号通路中Akt、p-Akt、PI3K、p-PI3K及对S100A4蛋白表达量的影响。结果 HBXIP在ACC-2中高表达,HBXIP-siRNA成功转染细胞株。MTT结果显示,48、72、96 h时实验组中存活的细胞数低于对照组,差异有统计学意义(P < 0.05);划痕实验结果显示,实验组细胞迁移率明显低于对照组(P < 0.01);transwell小室实验结果显示,实验组中细胞侵袭个数明显低于对照组(P < 0.01);蛋白印迹结果显示,相对于对照组,实验组细胞中p-Akt、p-PI3K及S100A4的表达量随着HBXIP基因沉默而相对降低。结论 HBXIP可促进腺样囊性癌细胞株ACC-2增殖、迁移和侵袭,其机制可能与促进Akt、PI3K磷酸化及增加S100A4蛋白表达量有关。

关键词: 乙肝病毒X蛋白结合蛋白, 腺样囊性癌细胞株ACC-2, 唾液腺, 生物学功能, PI3K/Akt信号通路

Abstract: Objective To study the effect of hepatitis B virus X-interacting protein (HBXIP) on the proliferation,migration,and invasion of ACC-2 cells,and the possible mechanism of the PI3K/Akt signaling pathway involved. Methods The chemically synthesized HBXIP-siRNA plasmid was transfected into the ACC-2 cells. RT-PCR and Western blotting were performed to detect the expression of HBXIP in the ACC-2 cells. Cell proliferation was measured via MTT assay. The invasive and migratory abilities of the ACC-2 cells were evaluated via the transwell chamber assay and scratch test,respectively. Western blotting also detected the impact of HBXIP-siRNA on Akt,p-Akt,PI3K,p-PI3K,and S100A4 protein expression. Results HBXIP was highly expressed and HBXIP-siRNA was successfully transfected in ACC-2 cells. MTT results showed that the number of surviving cells in the experimental group was significantly lower than that in the control group (P < 0.05). The scratch test results showed that the mobility of the experimental group was significantly lower than that of the control group (P < 0.01). The transwell assay showed that the rate of cell invasion of the experimental group was significantly lower than that of the control group (P < 0.01). Finally,Western blotting results revealed that the expression of p-Akt,p-PI3K,and S100A4 was relatively decreased in the experimental group when compared to that in the control group. Conclusion Silencing the HBXIP gene inhibited ACC-2 proliferation,invasion,and migration.

Key words: hepatitis B X-interacting protein, ACC-2 cell line, salivary gland, biological functions, PI3K/Akt signaling pathway

中图分类号: 

  • R782.7
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