中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2017, Vol. 46 ›› Issue (12): 1111-1116.doi: 10.12007/j.issn.0258-4646.2017.12.012

• 论著 • 上一篇    下一篇

人肝细胞癌细胞DNA甲基化谱的检测及分析

孙宁, 张佳林, 张城硕, 周翔宇, 陈保民   

  1. 中国医科大学附属第一医院肝胆外科暨器官移植科, 沈阳 110001
  • 收稿日期:2017-03-28 出版日期:2017-12-30 发布日期:2017-12-08
  • 通讯作者: 张佳林 E-mail:jlzcmu@126.com
  • 作者简介:孙宁(1986-),女,医师,博士.
  • 基金资助:
    沈阳市科学技术计划(F13-F13-212-9-00)

Exploring Genome-wide Profiles of DNA Methylation in Human Hepatocellular Carcinoma Cells via Bioinformatics Analysis

SUN Ning, ZHANG Jialin, ZHANG Chengshuo, ZHOU Xiangyu, CHEN Baomin   

  1. Department of Hepatobiliary and Transplantation Surgery, The First Hospital, China Medical University, Shenyang 110001, China
  • Received:2017-03-28 Online:2017-12-30 Published:2017-12-08

摘要: 目的 检测人肝细胞癌细胞的DNA甲基化谱,明确肝细胞癌细胞中差异甲基化位点和基因的表达分布情况,进一步探讨DNA异常甲基化与肝细胞癌发生发展的关系。方法 使用DNA甲基化芯片(Infinium Human Methylation 450K BeadChip)检测人肝细胞癌细胞Huh7和人永生化肝细胞L02的甲基化谱,并对检测结果进行生物学分析。结果 共检测到差异性甲基化位点102 254个,差异性甲基化基因26 511个,甲基化相关信号通路43个,其中57.3%的高甲基化CpG位点和39.4%的低甲基化CpG位点的甲基化差异程度≥ 50%,筛选后确定了3 222个显著高甲基化基因和2 204个显著低甲基化基因。结论 在Huh7和L02细胞中存在大量差异性甲基化CpG位点及基因,Huh7细胞中可检测到大量抑癌基因DNA的异常高甲基化,提示DNA异常甲基化与肝细胞癌的发生发展密切相关。

关键词: 肝细胞癌, 甲基化谱, 生物信息学分析

Abstract: Objective To detect the genome-wide profiles of DNA methylation in human hepatocellular carcinoma (HCC) cells and to identify the distribution of differentially methylated sites and genes in order to explore the relationship between aberrant DNA methylation and hepatocellular carcinoma. Methods The Infinium Human Methylation 450K BeadChip was used to identify the genome-wide aberrant DNA methylation profiles in Huh7 and L02 cell lines. Results Totally 102 254 differentially methylated CpG sites and 26 511 genes,involving 43 signaling pathways,were detected when Huh7 and L02 cell lines were compared. The absolute β-difference in 57.3% of the hypermethylated CpG sites and 39.4% of the hypomethylated CpG sites was reported to be ≥ 50%. A total of 3 222 hypermethylated genes and 2 204 hypomethylated genes were identified. Conclusion We detected many aberrant methylated sites and genes in HCC cells. The abnormal DNA methylation exhibits an important role in the occurrence and development of HCC.

Key words: hepatocellular carcinoma, methylation profiles, bioinformatics analysis

中图分类号: 

  • R735.7
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