中国医科大学学报

中国医科大学学报
  • 中文核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
  • BA、CA收录

中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (1): 9-12.doi: 10.12007/j.issn.0258-4646.2018.01.003

• 论著 • 上一篇    下一篇

结肠癌组织中miR-98的表达及作用机制

王俊江, 周天羽, 张扬, 刘明欣   

  1. 辽宁中医药大学附属医院肛肠科, 沈阳 110032
  • 收稿日期:2017-04-19 出版日期:2018-01-30 发布日期:2017-12-23
  • 通讯作者: 王俊江 E-mail:wyfdhg@163.com
  • 作者简介:王俊江(1977-),男,副主任医师,硕士.
  • 基金资助:
    国家自然科学基金(81202688)

Expression and Action Mechanism of miR-98 in Colon Cancer Tissues

WANG Junjiang, ZHOU Tianyu, ZHANG Yang, LIU Mingxin   

  1. Department of Anorectal Medicine, The Affiliated Hospital, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, China
  • Received:2017-04-19 Online:2018-01-30 Published:2017-12-23

摘要: 目的 探讨结肠癌组织中miR-98的表达及作用机制。方法 搜集40例结肠癌患者的肿瘤组织及癌旁组织标本,实时PCR检测miR-98在组织中的表达。在HCT116细胞中分别过表达/沉默miR-98后,MTT实验观察miR-98对其增殖的影响;流式细胞技术分析miR-98对细胞周期的影响;Hochest 33258染色判断miR-98对其凋亡的影响。结果 实时PCR检测发现,与癌旁组织比较,miR-98在肿瘤中低表达(P=0.022);生存分析发现miR-98表达低的患者5年生存率较低(P < 0.05)。MTT检测发现miR-98可以抑制细胞增殖。miR-98过表达可以抑制细胞周期进程,促进HCT116细胞凋亡。结论 miR-98可抑制结肠癌细胞增殖、促进细胞凋亡。miR-98表达与结肠癌患者的生存期密切相关。

关键词: miR-98, 结肠癌, 增殖, 凋亡, 生存期

Abstract: Objective To investigate the expression and action mechanism of miR-98 in colon cancer tissues. Methods Tumor tissues and adjacent tissues were collected from 40 patients with colorectal cancer. The expression of miR-98 in tumor tissues and adjacent tissues was detected by real-time PCR. miR-98 was overexpressed or silenced in cells,and the effects on proliferation,cell cycle,and apoptosis were analyzed using MTT,flow cytometry,and Hoechst 33258 assays. Results Real-time PCR showed that the expression of miR-98 in tumor tissues was lower than that in adjacent tissues (P=0.022). The survival rate of patients with lower miR-98 expression was shorter than that of patients with higher miR-98 expression. The MTT assay showed that miR-98 overexpression inhibited the proliferation of HCT116 cells. Hoechst 33258 staining showed that the overexpression of miR-98 could inhibit the cell cycle and promote the apoptosis of HCT116 cells. Conclusion miR-98 can inhibit the proliferation and promote the apoptosis of colon cancer cells. The expression of miR-98 is closely related to the survival of patients with colon cancer.

Key words: miR-98, colon cancer, proliferation, apoptosis, survival duration

中图分类号: 

  • R730.5
[1] FANTINI S,SALSI V,REGGIANI L,et al. The miR-196b miRNA inhibits the GATA6 intestinal transcription factor and is upregulated in colon cancer patients[J]. Oncotarget,2017,8(3):4747-4759. DOI:10.18632/oncotarget.13580.
[2] LI C,XU N,LI YQ,et al. Inhibition of SW620 human colon cancer cells by upregulating miRNA-145[J]. World J Gastroenterol,2016,22(9):2771-2778. DOI:10.3748/wjg.v22.i9.2771.
[3] PRADHAN M,NAGULAPALLI K,LEDFORD L,et al. A system biology approach for understanding the miRNA regulatory network in colon rectal cancer[J]. Int J Data Min Bioinform,2015,11(1):1-30.
[4] RAMALINGAM S,SUBRAMANIAM D,ANANT S. Manipulating miRNA expression:a novel approach for colon cancer prevention and chemotherapy[J]. Curr Pharmacol Rep,2015,1(3):141-153. DOI:10.1007/s40495-015-0020-3.
[5] SHA D,LEE AM,SHI Q,et al. Association study of the let-7 miRNA-complementary site variant in the 3' untranslated region of the KRAS gene in stage Ⅲ colon cancer (NCCTG N0147 clinical trial)[J]. Clin Cancer Res,2014,20(12):3319-3327. DOI:10.1158/1078-0432.CCR-14-0069.
[6] AHMED FE. miRNA as markers for the diagnostic screening of colon cancer[J]. Expert Rev Anticancer Ther,2014,14(4):463-485. DOI:10.1586/14737140.2014.869479.
[7] WENDLER A,KELLER D,ALBRECHT C,et al. Involvement of let-7/miR-98 microRNAs in the regulation of progesterone receptor membrane component 1 expression in ovarian cancer cells[J]. Oncol Rep,2011,25(1):273-279.
[8] KUMAZAKI M,NOGUCHI S,YASUI Y,et al. Anti-cancer effects of naturally occurring compounds through modulation of signal transduction and miRNA expression in human colon cancer cells[J]. J Nutr Biochem,2013,24(11):1849-1858. DOI:10.1016/j.jnutbio.2013.04.006.
[9] KANEMATSU S,TANIMOTO K,SUZUKI Y,et al. Screening for possible miRNA-mRNA associations in a colon cancer cell line[J]. Gene,2014,533(2):520-531. DOI:10.1016/j.gene.2013.08.005.
[10] LI B,SONG Y,LIU TJ,et al. miRNA-22 suppresses colon cancer cell migration and invasion by inhibiting the expression of T-cell lymphoma invasion and metastasis 1 and matrix metalloproteinases 2 and 9[J]. Oncol Rep,2013,29(5):1932-1938. DOI:10.3892/or.2013.2300.
[11] FANG Y,XIANG J,CHEN Z,et al. miRNA expression profile of colon cancer stem cells compared to non-stem cells using the SW1116 cell line[J]. Oncol Rep,2012,28(6):2115-2124. DOI:10.3892/or.2012.2054.
[12] WANG XY,WU MH,LIU F,et al. Differential miRNA expression and their target genes between NGX6-positive and negative colon cancer cells[J]. Mol Cell Biochem,2010,345(1/2):283-290. DOI:10.1007/s11010-010-0582-7.
[1] 曹勇, 王东旭, 孙妍, 杨俊, 张营, 郑长清. miR-26a在溃疡性结肠炎中的表达及对自噬的影响[J]. 中国医科大学学报, 2018, 47(9): 807-811.
[2] 岳媛, 范秋灵, 都姝妍, 远方, 徐莉, 李琳, 刘楠, 姜奕, 王力宁. 乌索酸对高糖诱导人系膜细胞损伤的作用[J]. 中国医科大学学报, 2018, 47(8): 682-686.
[3] 张怡, 林冰, 陈剑锋. 人脐带干细胞治疗重度烧伤大鼠急性肝损伤的实验研究[J]. 中国医科大学学报, 2018, 47(8): 687-691.
[4] 宋起滨, 王哲, 王宇令, 李杨, 林爽. 脂肪源性干细胞促进大鼠神经施万细胞增殖和分泌功能[J]. 中国医科大学学报, 2018, 47(6): 527-531.
[5] 李忠华, 李慧峰, 李晓蕾. 瞬时感受器电位香草酸受体3促进胶质瘤U251细胞系增殖和侵袭[J]. 中国医科大学学报, 2018, 47(5): 394-397.
[6] 马刚, 刘宁, 徐亮, 刘哲, 宋从浩, 王丹璞. 程序性细胞死亡因子4与5-氟尿嘧啶对胰腺癌细胞凋亡的协同作用[J]. 中国医科大学学报, 2018, 47(5): 431-434.
[7] 范一博, 车晓芳, 赵欢, 胡雪君, 曲秀娟, 刘云鹏. microRNA-1183通过抑制CBL-B的表达促进胃癌细胞的增殖及转移[J]. 中国医科大学学报, 2018, 47(3): 202-205,211.
[8] 宫雪, 于柳, 祝兴旺, 刘嘉, 刘屹立, 王平. 抑制Rab11表达对人膀胱癌细胞增殖和侵袭的影响[J]. 中国医科大学学报, 2018, 47(3): 247-250,259.
[9] 于婷, 王小梅. 通心络对高糖培养下心肌成纤维细胞增殖和凋亡的影响[J]. 中国医科大学学报, 2018, 47(2): 132-136.
[10] 窦磊, 庞晓燕, 李奇, 尚海, 张颐. 去酰基化ghrelin抑制上皮性卵巢癌细胞的增殖[J]. 中国医科大学学报, 2018, 47(12): 1102-1106.
[11] 贡鸣, 何天宇, 裘敬平, 李光. 6种常见肿瘤患者初治时外周血T淋巴细胞亚群数量及临床特征[J]. 中国医科大学学报, 2018, 47(12): 1119-1122.
[12] 郭冰玉, 回蔷, 常鹏, 陶凯. miR-489抑制黑色素瘤细胞的增殖[J]. 中国医科大学学报, 2018, 47(11): 1029-1031,1042.
[13] 柴芳, 李岩, 刘克毅, 李强. 长链非编码RNA XIST对肝癌细胞增殖及侵袭的影响[J]. 中国医科大学学报, 2018, 47(10): 895-899.
[14] 裴祎, 张晓晶, 郑珂, 王巍, 邱恩铎, 李盛龙. miR-212抑制骨肉瘤细胞恶性生物学行为的实验研究[J]. 中国医科大学学报, 2018, 47(10): 924-928.
[15] 尹建国, 张社兵, 彭道泉. 载脂蛋白AI通过诱导自噬抑制泡沫细胞脂质沉积和凋亡[J]. 中国医科大学学报, 2018, 47(1): 17-21.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!

中国医科大学学报版权所有©2018

未经允许,严禁擅自转载本站图文资料

地址:中国 沈阳市沈北新区蒲河路77号 110122

辽ICP备05014850

JOURNAL OF CHINA MEDICAL UNIVERSITY

ADDRESS: NO.77 PUHE ROAD

SHENYANG NORTH NEW AREA, SHENYANG

LIAONING PROVINCE, P.R. CHINA