中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (2): 132-136.doi: 10.12007/j.issn.0258-4646.2018.02.009

• 论著 • 上一篇    下一篇

通心络对高糖培养下心肌成纤维细胞增殖和凋亡的影响

于婷1, 王小梅2   

  1. 1. 锦州医科大学研究生学院, 辽宁 锦州 121001;
    2. 锦州医科大学附属第一医院老年医学科特需病房, 辽宁 锦州 121001
  • 收稿日期:2017-06-10 出版日期:2018-02-28 发布日期:2018-01-11
  • 通讯作者: 王小梅 E-mail:wxm631229@163.com
  • 作者简介:于婷(1990-),女,硕士研究生.
  • 基金资助:
    辽宁省科学技术计划(2009225010-42)

Effects of Tongxinluo on High Glucose-induced Proliferation and Apoptosis of Cardiac Fibroblasts

YU Ting1, WANG Xiaomei2   

  1. 1. Graduate School of Jinzhou Medical University, Jinzhou 121001, China;
    2. Special Needs Ward, Geriatrics Department, The First Affiliated Hospital of Jinzhou Medical University, Jinzhou 121001, China
  • Received:2017-06-10 Online:2018-02-28 Published:2018-01-11

摘要: 目的 观察通心络对高糖培养下心肌成纤维细胞增殖和凋亡的影响,探讨其抑制心肌纤维化的可能机制。方法 原代和传代培养SD大鼠乳鼠的心肌成纤维细胞,并用免疫荧光染色法鉴定。细胞分为对照组(低糖DMEM培养)、模型组(高糖DMEM培养)和通心络处理组(高糖DMEM+通心络20、80、320 μg/mL培养)。MTT检测各组细胞的增殖活力;ELISA检测各组Ⅰ、Ⅲ型胶原蛋白的分泌;Western blotting检测各组bax和bcl-2蛋白的表达。结果 模型组细胞的增殖活力及分泌的Ⅰ、Ⅲ型胶原蛋白均高于对照组,而通心络处理组细胞的增殖活力和分泌的胶原蛋白明显低于模型组;与对照组相比,模型组的bcl-2表达明显增多,bax表达减少;与模型组相比,通心络处理组的bcl-2表达减少,bax表达增加。结论 通心络可以减弱高糖培养下心肌成纤维细胞的增殖,并抑制其过度分泌Ⅰ、Ⅲ型胶原蛋白,同时还可通过减少bcl-2、增加bax的表达诱导心肌成纤维细胞凋亡,从而发挥其治疗心肌纤维化的作用。

关键词: 心肌成纤维细胞, 高糖, 通心络, 增殖, 凋亡

Abstract: Objective This study investigated the effect of tongxinluo (TXL) on high glucose-induced proliferation and apoptosis of cardiac fibroblasts (CFs),to explore the possible mechanism by which TXL inhibits myocardial fibrosis.Methods Primary culture and subculture of neonatal SD rat CFs was carried out as follows. Immunofluorescence staining was performed to identify CFs. The CFs were divided into control group (cultured with low-glucose DMEM),model group (cultured with high-glucose DMEM),and TXL treatment groups (cultured with high-glucose DMEM+TXL at 20,80,and 320 μg/mL). The proliferation of CFs in each group was detected by MTT assay. The expression of collagen typesⅠand Ⅲ in each group was detected by ELISA. Western blotting was used to detect the expression of bax and bcl-2 in each group.Results CFs Proliferation and collagen typesⅠandⅢsecretion were higher in the model group than in the control group. The CFs proliferation and collagen content in the TXL treatment groups were significantly lower than those in the model group. bcl-2 expression was significantly increased and bax expression was decreased in the model group,compared with the corresponding values in the control group. In comparison with the model group,bcl-2 expression was downregulated and bax expression was upregulated in the TXL treatment groups.Conclusion TXL can reduce the CFs proliferation and inhibit their collagen secretion under high glucose conditions. TXL also induces the CFs apoptosis by reducing bcl-2 expression and increasing bax expression. Thus,TXL can play a role in the treatment of myocardial fibrosis.

Key words: cardiac fibroblasts, highglucose, tongxinluo, proliferation, apoptosis

中图分类号: 

  • R54
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