中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (2): 145-150.doi: 10.12007/j.issn.0258-4646.2018.02.012

• 论著 • 上一篇    下一篇

离子型及Ⅰ型代谢型谷氨酸受体对大鼠温度过敏的调节作用

谢俐萍, 金幼虹, 叶芳, 罗玉, 杨娟霞, 陈霞   

  1. 南昌大学附属口腔医院牙周科, 江西省口腔生物医学重点实验室, 南昌 330006
  • 收稿日期:2017-07-11 出版日期:2018-02-28 发布日期:2018-01-11
  • 通讯作者: 金幼虹 E-mail:yhjin3713@sina.com
  • 作者简介:谢俐萍(1992-),女,医师,硕士研究生.
  • 基金资助:
    江西省科学技术厅重点研发计划(20161BBG70150);江西省卫生厅科技计划(20175297)

Regulatory Effects of Ionotropic and GroupⅠ Metabotropic Glutamate Receptors on Temperature Hypersensitivity in Rats

XIE Liping, JIN Youhong, YE Fang, LUO Yu, YANG Juanxia, CHEN Xia   

  1. Department of Periodontology, Affiliated Stomatological Hospital of Nanchang University, Jiangxi Key Laboratory of Stomatology, Nanchang 330006, China
  • Received:2017-07-11 Online:2018-02-28 Published:2018-01-11

摘要: 目的 探讨离子型及Ⅰ型代谢型谷氨酸受体对大鼠温度过敏的调节作用。方法 将离子型谷氨酸受体作用药谷氨酸(Glu)、N-甲基-D-天冬氨酸(NMDA)、α-氨基-3羟基-5甲基-4异恶唑(AMPA),Ⅰ型代谢型谷氨酸受体作用药[(S)-DHPG],非竞争性NMDA受体拮抗剂(MK-801),竞争性AMPA受体拮抗剂(CNQX),选择性Ⅰ型代谢型谷氨酸受体拮抗剂(cpccoEt)分别注入L5~6脊神经结扎和假手术大鼠左足底部皮下组织,观察大鼠对辐射热的反应。结果 Glu、NMDA、AMPA、(S)-DHPG能减少假手术组足底逃避阈值,而对手术组则无效;相反,MK-801、CNQX及cpccoEt能增加手术组足底逃避阈值,但对假手术组无效。结论 外周离子型和Ⅰ型代谢型谷氨酸受体敏感性的改变可导致末梢神经可塑性变化,这种变化是神经损伤引发的神经病理性疼痛产生与维持的基础。

关键词: L5~6脊神经结扎, 离子型谷氨酸受体, Ⅰ型代谢型谷氨酸受体, 温度过敏

Abstract: Objective To study the effects of ionic and group Ⅰ metabotropic glutamate receptors on rats' thermal hypersensitivity by intraplantar administration of drugs.Methods After intraplantar administration of glutamate receptor agonists,L-glutamic acid (Glu),N-methyl-D-aspartic-acid (NMDA),and (RS) -α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid hydrobromide (AMPA);a Group Ⅰ mGluR agonist, (S) 3,5-dihydroxyphenylglycine[(S) -DHPG];a noncompetitive NMDA receptor antagonist, (+) -MK801 maleate (MK-801);a competitive AMPA/kainate receptor antagonist,6-cyano-7-nitroquinoxaline-2,3-dione (CNQX);and a selective GroupⅠ mGluR antagonist,7-hydroxyiminocyclo propan[b]chromen-1a-carboxylic acid ethyl ester (cpccoEt) into the left hind paws of rats whose L5-6 nerves were sham-operated or ligated,we examined the response of the rats to thermal stimuli provided by radiant heat.Results In sham-operated rats,glutamate,NMDA,AMPA,and (S) -DHPG reduced paw withdrawal latency (PWL) but did not have any effect on SNL rats. However,in SNL rats,MK-801,CNQX,and cpccoEt increased PWL but exerted no effect on sham-operated rats.Conclusion These results suggest that changes in sensitivity of peripheral ionic and group Ⅰ metabotropic glutamate receptors can lead to changes in peripheral nerve plasticity;the generation and maintenance of neuropathic pain caused by nerve injury is based on this plasticity.

Key words: L5-6 spinal nerve ligation, ionotropic glutamate receptors, groupⅠmetabotropic glutamate receptors, temperature hypersensitivity

中图分类号: 

  • R741.02
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[1] 李晓倩,包娜仁,张再莉,马虹. 大麻素受体 1 对坐骨神经结扎大鼠神经病理性疼痛的影响及机制[J]. 中国医科大学学报, 2017, 46(3): 205-209.
[2] 于诗嘉,冯娟. 叶酸水平与帕金森病病情进展关系分析[J]. 中国医科大学学报, 2014, 43(8): 694-697.
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