中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (2): 157-161.doi: 10.12007/j.issn.0258-4646.2018.02.014

• 论著 • 上一篇    下一篇

小鼠肝硬化-肝癌上皮间质转化的研究

孙洪利   

  1. 中国医科大学附属盛京医院胆道血管外科, 沈阳 110004
  • 收稿日期:2017-03-27 出版日期:2018-02-28 发布日期:2018-01-11
  • 通讯作者: 孙洪利 E-mail:sun_hongli1@sina.com
  • 作者简介:孙洪利(1968-),男,副主任医师,博士.
  • 基金资助:
    沈阳市科学技术计划(F14-158-9-48)

Study of the Epithelial Mesenchymal Transition between Liver Cirrhosis and Liver Cancer in Mice

SUN Hongli   

  1. Department of Biliary Vascular Surgery, Shengjing Hospital, China Medical University, Shenyang 110004, China
  • Received:2017-03-27 Online:2018-02-28 Published:2018-01-11

摘要: 目的 通过建立动物模型,观察肝硬化及肝癌上皮间质转化过程。方法 建立小鼠肝硬化、肝癌动物模型,进行HE染色和免疫组化染色。结果 存活小鼠肝硬化诱导成功率100%,病死率30%。存活小鼠肝癌诱导成功率100%,病死率40%。在肝硬化和肝癌动物模型中,E-钙黏蛋白阳性表达率为46.7%和7.1%,β3整联蛋白阳性表达率为40.0%和7.1%,波形蛋白阳性表达率为13.6%和40.0%,纤维连接蛋白阳性表达率为20.0%和57.1%。结论 小鼠肝硬化和肝癌组织模型中,E-钙黏蛋白、β3整联蛋白的低表达和波形蛋白、纤维连接蛋白的高表达与肝硬化向肝癌的上皮间质转化过程有关,为临床检测的重要参照指标。

关键词: 肝硬化模型, 肝癌模型, E-钙黏蛋白, β3整联蛋白, 波形蛋白, 纤维连接蛋白, 上皮间质转化

Abstract: Objective To establish animal models facilitating observation of the process of epithelial-mesenchymal transition between liver cirrhosis and liver cancer.Methods Liver cirrhosis and liver cancer models were established in mouse and subsequently assessed using HE and immunohistochemical staining.Results The success rate of the liver cirrhosis model was 100% and the fatality rate was 30%. The success rate of the liver cancer model was also 100% and the fatality rate was 40%. In the liver cirrhosis and liver cancer models,the positive expression rates were,respectively,46.7% and 7.1% for E-cadherin,40.0% and 7.1% for β3-integrin,13.6% and 40.0% for vimentin,and 20.0% and 57.1% for fibronectin.Conclusion In mouse models of liver cirrhosis and liver cancer,low expression levels of E-cadherin and β3-integrin in addition to high expression levels of fibronectin and vimentin are related to the epithelial-mesenchymal transition between liver cancer and cirrhosis,and may be important indicators for clinical detection.

Key words: liver cirrhosis model, liver cancer model, E-cadherin, β3-integrin, vimentin, fibronectin, epithelial-mesenchymal transition

中图分类号: 

  • R735.7
[1] MICALIZZI DS,FARABAUGH SM,FORD HL. Epithelial mesenchymal transition in cancer:parallels between normal development and tumor progression[J]. Mammary Gland Biol Neoplasia,2010,15(2):117-134. DOI:10.1007/s10911-010-9178-9.
[2] ZENG Q,LI W,LU D,et al. CD146,an epithelial-mesenchymal transition inducer,is associated with triple-negative breast cancer[J]. Proc Natl Acad Sci U S A,2012,109(4):1127-1132. DOI:10.1073/pnas.1111053108.
[3] 叶燕丽,周听熙,王莲桂. 小鼠的繁殖及在肿瘤学中的应用[J]. 实验动物科学与管理,2005,22(1):6-8. DOI:10.3969/j.issn.1006-6179.2005.01.004.
[4] 阎丽,柳婧美,乔伟,等. 肝硬化基础上大鼠肝癌模型的建立[J]. 第四军医大学学报,2007,28(13):45-46. DOI:10.3321/j.issn:1000-2790.2007.13.010.
[5] LEWIS-TUFFIN LJ,RODRIGUEZ F,GIANNINI C,et al. Misregulated E-cadherin expression associated with an aggressive brain tumorphenotype[J]. PLoS One,2010,5(10):e13665. DOI:10.1371/journal.pone.0013665.
[6] SUN NX,YE C,ZHAO Q,et al. Long noncoding RNA-EBIC promotes tumor cell invasion by binding to EZH2 and repressing E-cadherin in cervical cancer[J]. PLoS One,2014,9(7):e100340. DOI:10.1371/journal.pone.0100340.
[7] KHARE V,LANG M,DAMMANN K,et al. Modulation of N-glycosylation by mesalamine facilitates membranous E-cadherin expression in colon epithelial cells[J]. Biochem Pharmacol,2014,87(2):312-320. DOI:10.1016/j.bcp.2013.10.021.
[8] JUSTIN M. DAVID,AYYAPPAN K,et al. Dishonorable discharge:the oncogenic roles of cleaved E-cadherin fragments[J]. Cancer Res,2012,72(12):2917-2923. DOI:10.1158/0008-5472.CAN-11-3498.
[9] PGLION F,ETIENNE-MANNEVILLE S. N-cadherin expression level as a critical indicator of invasion in non-epithelial tumors[J]. Cell Adh Migr,2012,6(4):327-332. DOI:10.4161/cam.20855.
[10] BALASUNDARAM P,SINGH MK,DINDA AK,et al. Study of β-catenin,E-cadherin and vimentin in oral squamous cell carcinoma with and without lymph node metastases[J]. Diagn Pathol,2014,9:145. DOI:10.1186/1746-1596-9-145.
[11] MENKO AS,BLEAKEN BM,LIBOWITZ AA,et al. A central role for vimentin in regulating repair function during healing of the lens epithelium[J]. Mol Biol Cell,2014,25(6):776-790. DOI:10.1091/mbc.E12-12-0900.
[12] HANDRA-LUCA A,HONG SM,WALTER K,et al. Tumour epithelial vimentin expression and outcome of pancreatic ductal adenocarcinomas[J]. Br J Cancer,2011,104(8):1296-1302. DOI:10.1038/bjc.2011.93.
[13] JING Y,JIA D,WONG CM,et al. SERPINA5 inhibits tumor cell migration by modulating the fibronectin-integrin β1 signaling pathway in hepatocellular carcinoma[J]. Mol Oncol,2014,8(2):366-377. DOI:10.1016/j.molonc.2013.12.003.
[14] SCHITTENHELM J,KLEIN A,TATAGIBA MS,et al. Comparing the expression of integrins αvβ3,αvβ5,αvβ6,αvβ8,fibronectin and fibrinogen in human brain metastases and their corresponding primary tumors[J]. Int J Clin Exp Pathol,2013,6(12):2719-2732.
[15] SHARMA M,TIWARI A,SHARMA S,et al. Fibrotic remodeling of the extracellular matrix through a novel (engineered,dual-function) antibody reactive to a cryptic epitope on the N-terminal 30 kDa fragment of fibronectin[J]. PLoS One,2013,8(7):e69343. DOI:10.1371/journal.pone.0069343.
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