中国医科大学学报

中国医科大学学报
  • 中文核心期刊
  • 中国科技核心期刊
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (3): 202-205,211.doi: 10.12007/j.issn.0258-4646.2018.03.003

• 论著 • 上一篇    下一篇

microRNA-1183通过抑制CBL-B的表达促进胃癌细胞的增殖及转移

范一博1, 车晓芳1, 赵欢2, 胡雪君2, 曲秀娟1, 刘云鹏1   

  1. 1. 中国医科大学附属第一医院肿瘤内科, 沈阳 110001;
    2. 中国医科大学附属第一医院呼吸疾病研究所老年病呼吸感染科, 沈阳 110001
  • 收稿日期:2017-08-23 出版日期:2018-03-30 发布日期:2018-03-24
  • 通讯作者: 刘云鹏 E-mail:cmuliuyunpeng@hotmail.com
  • 作者简介:范一博(1982-),女,讲师,博士.
  • 基金资助:
    国家自然科学基金(81602098);辽宁省科技厅计划(2014226033);辽宁省科技厅中央引导地方科技发展专项资金计划(2016007010)

microRNA-1183 Promotes the Proliferation and Metastasis by Suppressing the Expression of CBL-B in Gastric Cancer Cells

FAN Yibo1, CHE Xiaofang1, ZHAO Huan2, HU Xuejun2, QU Xiujuan1, LIU Yunpeng1   

  1. 1. Department of Medical Oncology, The First Hospital, China Medical University, Shenyang 110001, China;
    2. Institute of Respiratory Disease, Department of Respiratory and Infectious Disease of Geriatrics, The First Hospital, China Medical University, Shenyang 110001, China
  • Received:2017-08-23 Online:2018-03-30 Published:2018-03-24

摘要: 目的 研究microRNA-1183对胃癌细胞增殖及转移的影响,初步探讨microRNA-1183和CBL-B信号转导通路在此过程中的作用。方法 将人胃癌细胞MGC803瞬时转染microRNA模拟物microRNA-1183,采用实时PCR法检测胃癌细胞中microRNA-1183的表达,MTT法检测细胞增殖的能力,Transwell法检测细胞的转移。双荧光素酶报告基因检测系统检测microRNA-1183与CBL-B的结合,Western blotting观察蛋白的表达。结果 MTT法显示microRNA-1183可促进胃癌细胞MGC803的增殖,Transwell法显示microRNA-118促进了胃癌细胞MGC803的转移。BLAST对比分析结果显示,CBL-B是microRNA-1183的靶基因之一。Western blotting结果显示,过表达microRNA-1183后,CBL-B的表达明显下调。双荧光素酶报告基因检测系统证实CBL-B是microRNA-1183的靶基因。敲除CBL-B后,促进了MGC803的增殖与转移。microRNA-1183通过抑制CBL-B促进了胃癌细胞MGC803的增殖与转移。结论 microRNA-1183通过抑制CBL-B的表达,促进胃癌细胞MGC803的增殖及转移。

关键词: 胃癌, microRNA-1183, CBL-B, 增殖, 转移

Abstract: Objective To investigate the effect of microRNA-1183 on proliferation and metastasis on gastric cancer cells and to explore the role of microRNA-1183 and CBL-B signaling pathways in this process. Methods MGC803 cells were transfected with a microRNA-1183 mimic. Real-time PCR detected the expression of microRNA-1183 in gastric cancer cell line MGC803. MTT detected the proliferative effect of microRNA-1183 on MGC803 gastric cancer cells. A Transwell assay detected the effect of microRNA-1183 on the metastasis of MGC803 gastric cancer cells. A dual luciferase reporter assay detected the binding ability between microRNA-1183 and CBL-B. The expression of the protein was tested by Western blotting. Results MTT assay results showed that microRNA-1183 promoted the proliferation of MGC803 cells. Transwell assay results revealed that microRNA-1183 promoted the metastasis of MGC803 cells. The results of BLAST contrast analysis show that CBL-B is one of the target genes of microRNA-1183. Western blotting analysis showed that the mimic microRNA-1183 inhibited the expression of CBL-B. A dual luciferase reporter assay showed that CBL-B was the target gene of microRNA-1183. A CBL-B knockdown promoted the proliferation and metastasis of MGC803 cells. microRNA-1183 promoted the proliferation and metastasis of MGC803 cells by inhibiting the expression of CBL-B. Conclusion microRNA-1183 can inhibit the proliferation and metastasis of gastric cancer cell lines by inhibiting the expression of CBL-B.

Key words: gastric cancer cells, microRNA-1183, CBL-B, proliferation, metastasis

中图分类号: 

  • R735
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