中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (3): 206-211.doi: 10.12007/j.issn.0258-4646.2018.03.004

• 论著 • 上一篇    下一篇

Toll样受体4对大鼠脑缺血再灌注损伤的作用

邢海会, 丁晓慧, 解辉, 王忠华, 谢菊华, 陈丰洋, 罗崟洲, 周胜男   

  1. 沈阳医学院基础医学院组织学与胚胎学教研室, 沈阳 110034
  • 收稿日期:2017-08-21 出版日期:2018-03-30 发布日期:2018-03-24
  • 通讯作者: 丁晓慧 E-mail:1285448068@qq.com
  • 作者简介:邢海会(1987-),女,硕士研究生.
  • 基金资助:
    辽宁省教育厅科学研究课题一般项目(L2014406);国家级大学生创新创业训练计划(201510164006)

Effect of Toll-like Receptor 4 on Cerebral Ischemia Reperfusion Injury in Rats

XING Haihui, DING Xiaohui, XIE Hui, WANG Zhonghua, XIE Juhua, CHEN Fengyang, LUO Yinzhou, ZHOU Shengnan   

  1. Department of Histology and Embryology, College of Basic Medical Sciences, Shengyang Medical College, Shenyang 110034, China
  • Received:2017-08-21 Online:2018-03-30 Published:2018-03-24

摘要: 目的 探讨Toll样受体4(TLR4)在脑缺血再灌注损伤中的作用和机制。方法 将大鼠分为假手术组、缺血组(缺血+生理盐水)和缺血+TAK(TLR4拮抗剂)组,分别在术后1、3、7、14 d取材,进行形态学染色,并采用Western blotting检测TLR4及其调控因子核转录因子κB抑制蛋白激酶(IKKα/β)在缺血侧脑皮质区的表达。结果 缺血组和缺血+TAK组均出现不同程度的病理改变,术后1 d时缺血+TAK组重于缺血组,其余时间点均为缺血组更严重。TLR4在缺血侧脑组织的表达假手术组低于缺血组和缺血+TAK组;术后1和14 d,缺血组低于缺血+TAK组;术后3和7 d,缺血组高于缺血+TAK组。磷酸化的IKKα/β(P-IKKα/β)在缺血侧脑组织的表达术后1 d时缺血+TAK组高于缺血组,其余时间点均为缺血组表达量较高,假手术组表达量最低。结论 TLR4在早期(术后1 d)可减轻脑缺血再灌注损伤,而后期(术后3~14 d)可加重脑缺血再灌注损伤。TLR4发挥作用的机制可能是影响P-IKK α/β在缺血侧脑皮质的表达。

关键词: Toll样受体4, 缺血再灌注, 蛋白免疫印迹, 大脑, 大鼠

Abstract: Objective To explore the effect and mechanism of Toll-like receptor 4 (TLR4) on cerebral ischemia/reperfusion injury. Methods Rats were divided into a sham group,MCAO group,and MCAO+TAK group. Cerebral cortices were removed on day 1,3,7,and 14 post surgery. Morphological staining and Western blotting were used to detect pathological changes and TLR4 and PIKKα/β expression in brain tissues. Results The pathological changes in the MCAO+TAK group were more severe than in the MCAO group on day 1 post surgery. However,the MCAO group exhibited more severe damage at the other time points. TLR4 expression was lowest in the cerebral cortices of the sham group. On day 1 and 14 post surgery,TLR4 expression was lower in the MCAO group than in the MCAO+TAK group,while on day 3 and 7 post surgery,TLR4 expression was higher in the MCAO group than in the MCAO+TAK group. PIKKα/β expression was highest in the cerebral cortices of the MCAO group at all time points except for day 1. Conclusion TLR4 may alleviate cerebral ischemia reperfusion injury in rats on day 1 post surgery;however,TLR4 may exacerbate ischemia reperfusion injury 3 to 14 days post surgery. The mechanism may be due to the effect of PIKKα/β expression in the cerebral cortex.

Key words: Toll-like receptor 4, ischemia reperfusion, Western blotting, cerebral, rat

中图分类号: 

  • R743
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