中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (3): 226-230.doi: 10.12007/j.issn.0258-4646.2018.03.008

• 论著 • 上一篇    下一篇

白三烯受体拮抗剂通过Wnt/β-catenin信号通路影响哮喘小鼠气道重塑机制的研究

蒋昱1, 黄传君2, 李泽3, 张才擎2   

  1. 1. 山东大学医学院呼吸内科, 济南 250012;
    2. 山东省千佛山医院呼吸内科, 济南 250013;
    3. 临沂市高新技术产业开发区人民医院呼吸内科, 山东 临沂 276017
  • 收稿日期:2017-05-23 出版日期:2018-03-30 发布日期:2018-03-24
  • 通讯作者: 张才擎 E-mail:freezcq66@163.com
  • 作者简介:蒋昱(1985-),女,医师,硕士.
  • 基金资助:
    山东省自然科学基金(ZR2014HL003)

Effects of a Leukotriene Receptor Antagonist on Airway Remodeling in Asthmatic Mice via the Wnt/β-catenin Signaling Pathway

JIANG Yu1, HUANG Chuanjun2, LI Ze3, ZhANG Caiqing2   

  1. 1. Department of Respiratory Medicine, School of Medicine, Shandong University, Jinan 250012, China;
    2. Department of Respiratory Medicine, Qianfo Mountain Hospital, Jinan 250014, China;
    3. Department of Respiratory Medicine, Linyi High-tech Zone People's Hospital, Linyi 276017, China
  • Received:2017-05-23 Online:2018-03-30 Published:2018-03-24

摘要: 目的 探讨白三烯受体拮抗剂影响Wnt7b、β-catenin及c-Myc表达,干预哮喘小鼠气道重塑机制的研究。方法 采用卵蛋白(OVA)致敏和激发建立小鼠哮喘模型。将小鼠分为对照组、哮喘组以及孟鲁司特钠组。HE染色观察各组小鼠气道炎症情况;ELISA法检测血清OVA-sIgE水平;实时PCR和Western blotting分别定量分析肺组织中Wnt7b、β-cateninc-Myc mRNA和蛋白表达水平;运用图像采集系统测定支气管管腔周长(PBM)、支气管管壁面积(WAt)、支气管管壁平滑肌面积(WAm)和平滑肌细胞计数(N),上述指标用PBM标准化。结果 哮喘组小鼠血清OVA-sIgE表达水平明显升高(P < 0.05)。Western blotting以及实时PCR结果显示哮喘组Wnt7b、β-catenin及c-Myc蛋白和mRNA较对照组和孟鲁司特钠组表达均显著升高(P < 0.05)。气道重塑指标WAt/PBM、WAm/PBM孟鲁司特钠组均低于哮喘组(P < 0.05)。结论 Wnt/β-catenin信号通路可能是哮喘发病机制中的重要因素,白三烯受体拮抗剂(孟鲁司特钠)可能通过影响Wnt7b、β-catenin及c-Myc的表达干预哮喘气道重塑。

关键词: Wnt7b, β-catenin, c-Myc, 哮喘, 气道重塑, 白三烯受体拮抗剂

Abstract: Objective To investigate the expression of Wnt7b,β-catenin,and c-Myc in asthmatic mice and the intervention of the leukotriene receptor antagonist montelukast on airway remodeling. Methods The asthma model was established by ovalbumin (OVA) induction. HE staining was used to observe the pathological changes in lung tissue. Serum OVA-sIgE levels were determined by ELISA. The level of Wnt7b,β-catenin,and c-Myc protein and mRNA in the lung tissue of mice was analyzed by Western blotting and real-time PCR. The basement membrane perimeter (PBM),wall area of bronchial tube (WAt),wall area of bronchial smooth muscle (WAm),and the number of smooth muscle cells were measured using medical image analysis software and standardized based on the PBM. Results The amount of OVA-slgE in the asthma group was significantly higher than in the control and montelukast groups (P < 0.05). Western blotting and real-time PCR showed that the expression of Wnt7b,β-catenin,and c-Myc in the asthma group was higher than the expression in the control and montelukast groups (P < 0.05). Image analysis showed that the WAt/PBM and WAm/PBM ratios in the montelukast group were significantly lower than those in the asthma group (P < 0.05). Conclusion The Wnt/β-catenin signaling pathway may be an important factor in the pathogenesis of asthma;montelukast may attenuate airway remodeling in asthmatic patients by decreasing the expression of Wnt7b,β-catenin,and c-Myc.

Key words: Wnt7b, β-catenin, c-Myc, asthma, airway remodeling, leukotriene receptor antagonists

中图分类号: 

  • R562.25
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