中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (3): 231-236.doi: 10.12007/j.issn.0258-4646.2018.03.009

• 论著 • 上一篇    下一篇

参桂保心方对慢性心力衰竭大鼠基质金属蛋白酶2、基质金属蛋白酶抑制剂1、B型脑钠肽及心室重构的影响

卢玉俊, 赵信科, 代晶晶   

  1. 甘肃中医药大学附属医院心血管临床医学中心, 兰州 730000
  • 收稿日期:2017-08-29 出版日期:2018-03-30 发布日期:2018-03-24
  • 通讯作者: 卢玉俊 E-mail:jj_lu@163.com
  • 作者简介:卢玉俊(1982-),男,副主任医师,硕士.
  • 基金资助:
    甘肃省自然科学基金(148RJZA068)

Effects of ShenGuiBaoXin Decoction on Matrix Metalloproteinase-2,the Tissue Inhibitor of Matrix Metalloproteinase-1,Brain Natriuretic Peptide,and Ventricular Remodeling in Rats with Chronic Heart Failure

LU Yujun, ZHAO Xinke, DAI Jingjing   

  1. Cardiovascular Center, Affiliated Hospital of Gansu University of Chinese Medicine, Lanzhou 730000, China
  • Received:2017-08-29 Online:2018-03-30 Published:2018-03-24

摘要: 目的 观察参桂保心煎剂对心力衰竭大鼠基质金属蛋白酶2(MMP-2)、基质金属蛋白酶抑制剂1(TIMP-1)、B型脑钠肽(BNP)水平及其对心肌组织的影响,探讨该药对心室重构的抑制作用。方法 将90只SD大鼠分为2组,对照组15只,注射盐水;实验组75只,注射异丙肾上腺素3 mg/(kg·d),4周后射血分数 ≤ 50%为造模成功。将实验组大鼠分为模型组,芪苈强心组,参桂保心高、中、低剂量组,每组15只。8周后测定心胸比,BNP,心肌MMP-2、TIMP-1表达,并进行HE染色。结果 心胸比和BNP在治疗后均有下降,芪苈强心组及高剂量组尤为明显;MMP-2表达在治疗后均有减少,其中,高剂量组优于芪苈强心组。结论 参桂保心可能通过减少MMP-2表达,调节MMP-2/TIMP-1的动态平衡,维持细胞外基质合成与降解平衡,达到抑制心室重构的作用。

关键词: 慢性心力衰竭, 基质金属蛋白酶2, 基质金属蛋白酶抑制剂1, B型脑钠肽, 参桂保心方

Abstract: Objective To observe the effects of ShenGuiBaoXin decoction on matrix metalloproteinases-2 (MMP-2),the tissue inhibitor of matrix metalloproteinase-1 (TIMP-1),brain natriuretic peptide (BNP) levels,and cardiac histopathology in rats with heart failure,and to investigate the mechanism of the inhibition effect on ventricular remodeling in rats with heart failure. Methods Ninety male SD rats were randomly divided into two groups,with 15 rats in the control group and 75 rats in the model group. Normal saline was injected in the normal control group. The model group was given an intraperitoneal injection of isoprenaline 3 mg/(kg·d). After 4 weeks,an ejection fraction of ≤ 50% on echocardiography was considered a successful model. Seventy-five rats were randomly divided into the model,Qiliqiangxin,and ShenGuiBaoXin groups (high,medium,and low doses),with 15 rats in each group. Eight weeks later,the cardiothoracic ratio and BNP level were measured,hematoxylin-eosin staining was performed,and the MMP-2 and TIMP-1 protein expressions in the myocardium was detected. Results In terms of cardiothoracic ratio and BNP level,all the groups showed decreases after treatment,especially the Qiliqiangxin and high-dose groups. The MMP-2 protein expression level was decreased in all the groups after the treatment,and the high-dose group was superior to the Qiliqiangxin group. Conclusion ShenGuiBaoXin decoction functions by reducing the MMP-2 expression level,regulating MMP-2/TIMP-1 dynamic balance,maintaining the balance of synthesis and degradation of the extracellular matrix,inhibiting ventricular remodeling,and improving cardiac function.

Key words: chronic heart failure, matrix metalloproteinases-2, tissue inhibitor of matrix metalloproteinase-1, brain natriuretic peptide, ShenGuiBaoXin decoction

中图分类号: 

  • R765.2
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