中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (3): 247-250,259.doi: 10.12007/j.issn.0258-4646.2018.03.013

• 论著 • 上一篇    下一篇

抑制Rab11表达对人膀胱癌细胞增殖和侵袭的影响

宫雪, 于柳, 祝兴旺, 刘嘉, 刘屹立, 王平   

  1. 中国医科大学附属第四医院泌尿外科, 沈阳 110032
  • 收稿日期:2017-07-20 出版日期:2018-03-30 发布日期:2018-03-24
  • 通讯作者: 宫雪 E-mail:gongxue216@126.com
  • 作者简介:宫雪(1983-),女,主治医师,博士.
  • 基金资助:
    辽宁省科学技术计划(2015225009)

Effect of Low Rab11 Expression on the Proliferation and Invasion of Human Bladder Cancer Cells

GONG Xue, YU Liu, ZHU Xingwang, LIU Jia, LIU Yili, WANG Ping   

  1. Urinary Surgery Department, The Fourth Affiliated Hospital, China Medical University, Shenyang 110032, China
  • Received:2017-07-20 Online:2018-03-30 Published:2018-03-24

摘要: 目的 探讨抑制Rab11表达对膀胱癌T24细胞增殖和侵袭的影响。方法 将Rab11 siRNA转染于人膀胱癌T24细胞系,用免疫印迹法检测干扰效率。通过CCK8、细胞周期检测及基质胶侵袭实验,分析抑制Rab11表达对细胞增殖、细胞周期和侵袭的影响。通过免疫印迹法及实时PCR检测抑制Rab11表达对细胞周期相关因子cyclin D1、cyclin E及侵袭相关因子基质金属蛋白酶9(MMP9)表达量的影响。结果 抑制Rab11蛋白表达能够抑制膀胱癌细胞的增殖和侵袭,并下调细胞周期相关因子cyclin D1、cyclin E及侵袭相关因子MMP9的蛋白和mRNA表达量。结论 Rab11可能作为肿瘤蛋白参与膀胱癌的疾病进展。

关键词: Rab11, 膀胱尿路上皮癌, 增殖, 侵袭

Abstract: Objective To investigate the effect of inhibition of Rab11 expression on the proliferation and invasion of human bladder cancer cell line T24. Methods T24 cells were transfected with Rab11 siRNA,and RNA interference efficiency was determined by performing Western blotting. The effect of inhibition of Rab11 expression on cell proliferation,cell cycle progression,and cell invasion was analyzed by performing CCK8,cell cycle detection,and Matrigel invasion assays,respectively. Expression of cell cycle-related proteins cyclin D1 and cyclin E and invasion-related protein matrix metalloproteinase 9 (MMP9) was determined by performing Western blotting and RT-PCR. Results Inhibition of Rab11 expression inhibited the proliferation and invasion of bladder cancer cells and downregulated the expression of cell cycle-related proteins cyclin D1 and cyclin E and invasion-related protein MMP9. Conclusion Our results suggest that Rab11 functions as a tumor protein and is involved in the progression of bladder cancer.

Key words: Rab11, bladder urothelial carcinoma, proliferation, invasion

中图分类号: 

  • R737.14
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