中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (3): 251-255.doi: 10.12007/j.issn.0258-4646.2018.03.014

• 论著 • 上一篇    下一篇

哮喘患者气道上皮细胞关键基因的生物信息学分析

周冬梅1, 孔灵菲2   

  1. 1. 中国医科大学附属第一医院老年呼吸感染科, 沈阳 110001;
    2. 中国医科大学附属第一医院呼吸内科, 沈阳 110001
  • 收稿日期:2017-10-13 出版日期:2018-03-30 发布日期:2018-03-24
  • 通讯作者: 孔灵菲 E-mail:konglingf@sina.com
  • 作者简介:周冬梅(1986-),女,医师,硕士.
  • 基金资助:
    沈阳市科学技术计划(F13-318-1-33)

Identification of Key Genes of Airway Epithelial Cells in Asthma Patients by Bioinformatics-based Analysis

ZHOU Dongmei1, KONG Lingfei2   

  1. 1. Department of Respiratory and Infection in Elderly, The First Hospital, China Medical University, Shenyang 110001, China;
    2. Department of Respiratory, The First Hospital, China Medical University, Shenyang 110001, China
  • Received:2017-10-13 Online:2018-03-30 Published:2018-03-24

摘要: 目的 通过比较支气管哮喘患者气道上皮细胞与正常气道上皮细胞的基因芯片数据,鉴定哮喘气道上皮细胞中的关键基因。方法 对哮喘气道上皮细胞基因芯片数据GSE43696进行GEO2R分析,筛选出差异表达基因。然后使用DAVID数据库对差异表达基因进行功能及通路富集化分析。利用STRING对差异表达基因进行蛋白相互作用网络图(PPI)分析,并选出关键基因及显著模块。结果 共获得355个差异表达基因,130个上调基因,225个下调基因,主要涉及细胞运动、生长因子结合离子转运和通道活性。共筛出9个关键基因,包括BDNF,ERBB2,IL6,VEGFA,KIT,ADCY4,PRKAR2B,CCR6NMU结论 筛选的关键基因均在支气管哮喘中起重要作用,为支气管哮喘的诊治提供潜在的治疗目标。

关键词: 支气管哮喘, 气道上皮细胞, 差异表达基因, 基因本体分析, 蛋白相互作用网络图

Abstract: Objective We aimed to identify key genes and pathways of airway epithelial cells involved in bronchial asthma by comparing genetic information in the databases for patients with bronchial asthma and normal people. Methods To find differentially expressed genes (DEGs),mRNA microarray dataset,GSE43696,of airway epithelial cells in asthma was analyzed by GEO2R. Functional and pathway enrichment analyses were performed for DEGs using the DAVID database. The protein-protein interaction networks were established using STRING to identify key genes and important complexes. Results A total of 355 DEGs were identified;of which,130 were up-regulated and 225,down-regulated. The genes identified were involved in cell movement,growth factor binding,and ion channel activity. Nine key genes were recognized,including BDNF,ERBB2,IL6,VEGFA,KIT,ADCY4,PRKAR2B,CCR6,and NMU. Conclusion All nine key genes identified play important roles in asthma and serve as potential targets for treatment of bronchial asthma.

Key words: bronchial asthma, airway epithelial cell, differentially expressed genes, gene oncology, protein-protein interaction network

中图分类号: 

  • R562.25
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