中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (3): 268-274.doi: 10.12007/j.issn.0258-4646.2018.03.018

• 论著 • 上一篇    下一篇

促血管生成microRNA在冠状动脉疾病诊断中的作用及其与疾病严重程度的关系

郭观华, 黄自明, 陈丽萍   

  1. 湛江中心人民医院心内二科, 广东 湛江 524037
  • 收稿日期:2017-03-31 出版日期:2018-03-30 发布日期:2018-03-24
  • 通讯作者: 陈丽萍 E-mail:lpz0422@126.com
  • 作者简介:郭观华(1977-),男,副主任医师,本科.

Circulating Pro-angiogenic miRNAs in Predicting the Risk and Severity of Coronary Artery Disease

GUO Guanhua, HUANG Ziming, CHEN Liping   

  1. Department of Cardiology, Central People's Hospital of Zhanjiang, Zhanjiang 524037, China
  • Received:2017-03-31 Online:2018-03-30 Published:2018-03-24

摘要: 目的 探讨血管生成相关的microRNA(miRNA)在冠状动脉疾病(CAD)诊断中的作用及其与CAD严重程度的关系。方法 将研究分为探索阶段与验证阶段。在探索阶段研究中,纳入经血管造影确诊为CAD的患者10例作为实验组,同时纳入10例无CAD征象的健康受试者作为健康对照组(HCs),初步筛选CAD患者中的血管生成相关差异表达miRNA(DEMs);验证阶段研究中,纳入142例CAD患者以及140例HCs,入组及排除标准同探索阶段。采用Gensini评分系统评估CAD患者疾病严重程度。采集所有受试者的外周血样本,采用实时荧光定量PCR测定备选miRNA的相对表达量。结果 探索阶段结果显示,血浆miR-19a、miR-92a、miR-126、miR-130a、miR-210、miR-296和miR-378在CAD患者中的表达水平显著低于HCs,而CAD患者血浆miR-221的相对表达量显著高于HCs。验证阶段结果显示,CAD患者中miR-126、miR-130a、miR-210和miR-378的表达量显著低于HCs组。多元逻辑回归结果表明,miR-19a、miR-126、miR-130a、miR-210和miR-378的高表达水平是CAD的独立保护因素。结合miR-19a、miR-126、miR-130a、miR-210和miR-378的相对表达量检测,对于CAD具有较好的诊断价值,曲线下面积(AUC)为0.768。miR-126和miR-210的表达水平与Gensini评分呈负相关。结论 miR-19a、miR-126、miR-130a、miR-210和miR-378可以作为预测CAD发病风险和疾病严重程度的生物标志物。

关键词: 循环, 促血管生成, microRNA, 冠状动脉疾病

Abstract: Objective To evaluate the correlations of pro-angiogenic miRNAs with risk and severity in patients with coronary artery disease (CAD). Methods After angiographic documentation,10 patients with CAD and 10 healthy controls (HCs) were enrolled for screening the differently expressed microRNAs (DEMs) (exploration stage). Blood samples of 142 patients with CAD and 140 age-and gender-matched HCs with the same eligibility were collected (validation stage). And the plasma levels of DEMs were measured by real-time qPCR. Results In the exploration stage,miR-19a,miR-92a,miR-126,miR-130a,miR-210,miR-296,and miR-378 expression levels were higher,but miR-221 expression was lower in patients with CAD than in HCs. In the validation stage,plasma expression levels of miR-126,miR-130a,miR-210,and miR-378 were decreased in patients with CAD. Multivariate logistic regression revealed that miR-19a,miR-126,miR-130a,miR-210,and miR-378 were independent protective factors for CAD. Receiver operating characteristic (ROC) analysis displayed that testing of a combination of miR-19a,miR-126,miR-130a,miR-210,and miR-378 was of good value in predicting the risk of CAD,with an AUC of 0.768. Additionally,miR-126 and miR-210 were negatively associated with Gensini scores. Conclusion miR-126,miR-130a,miR-210,and miR-378 could serve as promising biomarkers for predicting the risk and severity of CAD.

Key words: circulating, pro-angiogenic, microRNA, coronary artery disease

中图分类号: 

  • R543.3
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