中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (4): 299-304.doi: 10.12007/j.issn.0258-4646.2018.04.003

• 论著 • 上一篇    下一篇

人激肽释放酶相关肽10 ELISA方法建立及其临床应用

杨帆1, 陈英剑2, 吴艳花2, 胡成进2   

  1. 1. 锦州医科大学研究生院, 辽宁 锦州 121001;
    2. 济南军区总医院实验诊断科, 济南 250031
  • 收稿日期:2017-06-16 出版日期:2018-04-30 发布日期:2018-04-10
  • 通讯作者: 胡成进 E-mail:hcj6289@163.com
  • 作者简介:杨帆(1990-),女,硕士研究生.
  • 基金资助:
    国家自然科学基金(81472497)

Establishment of a Method for Detecting Kallikrein 10 in the Serum and Its Clinical Application

YANG Fan1, CHEN Yingjian2, WU Yanhua2, HU Chengjin2   

  1. 1. Graduate School of Jinzhou Medical University, Jinzhou 121001, China;
    2. Department of Laboratory Medicine, General Hospital of Jinan Military Command Region, Jinan 250031, China
  • Received:2017-06-16 Online:2018-04-30 Published:2018-04-10

摘要: 目的 建立人激肽释放酶相关肽10(KLK10)双抗体夹心酶联免疫吸附试验(ELISA),并利用该方法初步探讨KLK10作为乳腺癌肿瘤标志物的临床价值。方法 建立KLK10双抗体夹心ELISA检测方法,对该方法进行方法学评估。采用本方法检测健康正常人群(健康对照组)、乳腺良性病女性患者、乳腺癌女性患者血清中KLK10含量,分析KLK10含量与乳腺癌临床病理特性的相关性及其临床应用价值。结果 利用KLK10 ELISA检测方法检测乳腺癌患者、乳腺良性病患者、健康对照组血清中KLK10含量分别为(0.88±0.45)、(1.35±0.56)、(1.57±0.88)ng/mL,3组比较具有统计学差异(均P < 0.05)。KLK10对乳腺癌具有较高的诊断灵敏度、准确率,其检测效能高于现有乳腺癌血清标志物。结论 成功建立了具有高灵敏度和特异性的KLK10双抗体夹心ELISA检测方法,可用于临床血清KLK10检测;KLK10与乳腺肿瘤的发生发展密切相关,有潜力成为乳腺肿瘤标志物。

关键词: 人激肽释放酶相关肽10, 乳腺癌, 血清标志物, ELISA, 诊断价值

Abstract: Objective To establish an ELISA method for detecting kallikrein 10 (KLK10) in the serum,and evaluate KLK10 as a biomarker for breast cancer. Methods The KLK10 ELISA assay was established and its efficiency was evaluated. Then,using this method, the serum KLK10 levels in all the samples were quantified. The correlation between KLK10 level and clinicopathological features,and the clinical value of KLK10 were analyzed. Results The serum KLK10 level in patients with breast cancer,patients with benign breast disease,and normal controls was found to be 0.88±0.45,1.35±0.56,and 1.57±0.88 ng/mL,respectively. The KLK10 levels in patients with breast cancer were significantly different from those in patients from the other groups (P < 0.05). The sensitivity,accuracy,and Youden index of KLK10 detection in the breast cancer samples were higher than those for CEA and CA 153 detection,which proved that KLK10 has potential as a new serum marker for breast cancer. Conclusion The KLK10 ELISA assay was established successfully and KLK10 could be detected in the serum samples. The presence of KLK10 in the serum was closely related to the occurrence and development of breast cancer,and KLK10 has potential as a marker for breast cancer.

Key words: kallikrein 10, breast cancer, serum marker, ELISA, diagnostic value

中图分类号: 

  • R446.6
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