中国医科大学学报

中国医科大学学报
  • 中文核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (4): 355-357,363.doi: 10.12007/j.issn.0258-4646.2018.04.015

• 论著 • 上一篇    下一篇

miR-489通过打靶PAK5抑制黑色素瘤的迁移

郭冰玉, 回蔷, 常鹏, 陶凯   

  1. 沈阳军区总医院整形外科, 沈阳 110840
  • 收稿日期:2017-04-21 出版日期:2018-04-30 发布日期:2018-04-10
  • 通讯作者: 陶凯 E-mail:luzongzhengxing@sina.com
  • 作者简介:郭冰玉(1989-),女,技师,硕士.

miR-489 Inhibits Melanoma Migration by Targeting PAK5

GUO Bingyu, HUI Qiang, CHANG Peng, TAO Kai   

  1. Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military Region, Shenyang 110840, China
  • Received:2017-04-21 Online:2018-04-30 Published:2018-04-10

摘要: 目的 探讨miR-489对黑色素瘤迁移的抑制作用。方法 通过Transwell实验检测miR-489对黑色素瘤迁移的影响;荧光素酶报告基因实验检测A375细胞中miR-489对PAK5的靶向作用;进一步通过Western blotting以及实时PCR检测miR-489对于A375细胞中PAK5的影响。结果 Transwell实验发现miR-489过表达后可以显著抑制A375细胞的迁移,当miR-489受到抑制后,A375细胞的迁移受到促进。荧光素酶报告基因实验证明,miR-489可以直接作用于PAK5。Western blotting和实时PCR检测发现,过表达miR-489可以抑制A375细胞中PAK5蛋白的表达;当miR-489受到抑制后蛋白表达有所增加。结论 miR-489可以通过打靶PAK5抑制黑色素瘤细胞的迁移。

关键词: miR-489, 黑色素瘤, A375, 迁移, PAK5

Abstract: Objective To investigate the role of miR-489 in inhibiting the migration of melanoma cells. Methods The effect of miR-489 on the migration of melanoma cells was detected using the Transwell assay. Luciferase reporter gene assay was used to detect the effect of miR-489 on PAK5 in A375 human melanoma cells. Western blotting and real-time PCR were performed to test the effect of miR-489 on PAK5 in A375 cells. Results The Transwell assay showed that overexpression of miR-489 could significantly inhibit the migration of A375 cells,and that the migration of A375 cells was promoted when miR-489 was inhibited. Luciferase reporter gene experiments demonstrated that miR-489 could act directly on PAK5. Western blotting and real time PCR assay showed that overexpression of miR-489 could inhibit the expression of PAK5 protein in A375 cells,and the expression of PAK5 was increased when miR-489 was inhibited. Conclusion miR-489 can inhibit the migration of melanoma cells by targeting PAK5.

Key words: miR-489, melanoma cancer, A375, migration, PAK5

中图分类号: 

  • R739.5
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