中国医科大学学报

中国医科大学学报
  • 中文核心期刊
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (5): 398-401,405.doi: 10.12007/j.issn.0258-4646.2018.05.004

• 论著 • 上一篇    下一篇

转化生长因子β1对人牙髓干细胞成纤维向分化的作用

姜力铭, 宋戈, 夏商, 陈旭   

  1. 中国医科大学口腔医学院儿童口腔科, 辽宁省口腔疾病重点实验室, 辽宁省口腔疾病转化医学研究中心, 沈阳 110002
  • 收稿日期:2017-11-24 出版日期:2018-05-30 发布日期:2018-04-27
  • 通讯作者: 陈旭 E-mail:chenxu_cmu@sina.com
  • 作者简介:姜力铭(1983-),女,讲师,博士.
  • 基金资助:
    国家自然科学基金(81771059);中国医科大学口腔医学院青年科技基金启动项目(K101593-15-05)

Effects of Transforming Growth Factor-β1 on the Fibrogenic Differentiation of Human Dental Pulp Stem Cells

JIANG Liming, SONG Ge, XIA Shang, CHEN Xu   

  1. Department of Pediatric Dentistry, School of Stomatology, China Medical University, Liaoning Provincial Key Laboratory of Oral Disease, Shenyang 110002, China
  • Received:2017-11-24 Online:2018-05-30 Published:2018-04-27

摘要: 目的 研究不同浓度转化生长因子β1(TGF-β1)促进人牙髓干细胞(hDPSCs)成纤维分化的作用。方法 利用含有不同浓度(1、5、10、20 ng/mL) TGF-β1的细胞培养基培养hDPSCs,分别在第1、3和7天时检测细胞增殖情况;在第7和14天时,采用RT-PCR方法检测成纤维相关因子[I型胶原蛋白(Collagen I)、纤连蛋白(Fibronectin)、骨膜蛋白(Periostin)和α平滑肌肌动蛋白(α-SMA)]的表达情况。结果 MTT结果显示,第7天时,TGF-β1(1 ng/mL和5 ng/mL)可明显促进hDPSCs增殖。RT-PCR结果表明:第7 d时,1ng/mL组、5 ng/mL组和10 ng/mL组Collagen I的表达水平分别为对照组的8.2倍、9.3倍和8.1倍,α-SMA的表达水平分别为对照组的6.2倍、6.7倍和5.8倍。5 ng/mL和10 ng/mL组的Periostin表达水平相近,约为1 ng/mL和20 ng/mL组的3.2倍和1.8倍(P < 0.05)。第14天时,各实验组的成纤维相关因子表达水平均下降,但仍高于对照组。结论 TGF-β1可以促进hDPSCs的成纤维分化,浓度为10ng/mL时效果最明显。

关键词: 转化生长因子β1, 人牙髓干细胞, 成纤维分化

Abstract: Objective To investigate the effects of transforming growth factor beta 1(TGF-β1) on promoting the fibrogenic differentiation of human dental pulp stem cells(hDPSCs).Methods The cells were treated with different concentrations of TGF-β1(1,5,10,and 20 ng/mL) and cell proliferation were examined after 1,3,and 7 days. The expression of fibrogenic-related factors(Collagen I,Fibronectin,Periostin,and α-SMA) was examined by RT-PCR at 7 and 14 days.Results MTT data showed that 1 and 5 ng/mL of TGF-β1 could promote the proliferation of hDPSCs at 7 days. RT-PCR results showed that under 1,5,and 10 ng/mL TGF-β1 treatment,the Collagen I expression was 8.2,9.3,and 8.1 times and theα-SMA expression was 6.2,6.7,and 5.8 times higher than that of the control group at 7 days. Periostin expression levels in the 5 and 10 ng/mL TGF-β1 groups were similar and about 3.2 and 1.8 times the expression levels of the 1 and 20 ng/mL groups,respectively(P < 0.05). At 14 days,the expression levels of the fibrogenic-related factors in the experimental group decreased but were still higher than those of the control group.Conclusion TGF-β1 can promote the fibrogenic differentiation of hDPSCs with the 10 ng/mL treatment being the most effective.

Key words: transforming growth factor β1, human dental pulp stem cell, fibrogenic differentiation

中图分类号: 

  • R781.3
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