中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (8): 678-681.doi: 10.12007/j.issn.0258-4646.2018.08.002
韩旭, 于潜, 田野, 赵希彤, 张磊, 周镝, 田大力
HAN Xu, YU Qian, TIAN Ye, ZHAO Xitong, ZHANG Lei, ZHOU Di, TIAN Dali
摘要: 目的 明确肺癌转移相关基因ABCE1编码蛋白与β-肌动蛋白的相互作用结构域。方法 通过转化技术,在E.coli BL21(DE3)中诱导表达前期研究获得的表达载体pGEX-4T-1-ABCE1-55,获得GST-ABCE1-55重组融合蛋白表达,应用GST pull-down的方法验证pGEX-4T-1-ABCE1-55与β-肌动蛋白有无相互作用。结果 GST-ABCE1-55是带有GST标签的含有357个氨基酸的重组蛋白,大小约66×103,在GST pull-down实验中,GST-ABCE1-55并不能与β-肌动蛋白特异结合。结论 肺癌转移相关基因ABCE1编码蛋白与β-肌动蛋白的相互作用结构域应位于ABCE1蛋白第358位至第600位编码氨基酸中。
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