中国医科大学学报

中国医科大学学报
  • 中文核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (9): 788-791,802.doi: 10.12007/j.issn.0258-4646.2018.09.005

• 论著 • 上一篇    下一篇

组蛋白去乙酰化酶6在慢性哮喘小鼠肺组织中的表达形态

任媛, 苏新明, 陆常玲, 李朋, 李孟露, 康健   

  1. 中国医科大学附属第一医院呼吸与危重症医学科, 沈阳 110001
  • 收稿日期:2018-03-16 出版日期:2018-09-30 发布日期:2018-09-08
  • 通讯作者: 苏新明 E-mail:xinming_s@163.com
  • 作者简介:任媛(1989-),女,讲师,博士.
  • 基金资助:
    国家自然科学基金(81770021);辽宁省自然科学基金(20170540994)

Histone Deacetylase 6 Expression Pattern in the Lung Tissue of Chronic Asthmatic Mice

REN Yuan, SU Xinming, LU Changling, LI Peng, LI Menglu, KANG Jian   

  1. Department of Respiratory and Critical Diseases, The First Hospital, China Medical University, Shenyang 110001, China
  • Received:2018-03-16 Online:2018-09-30 Published:2018-09-08

摘要: 目的 探讨组蛋白去乙酰化酶6(HDAC6)在慢性哮喘小鼠肺组织中的表达形态变化。方法 将BALB/C小鼠分为正常组和哮喘组,每组12只。应用卵蛋白致敏激发方法构建慢性哮喘模型。末次激发试验24 h后,收集各组小鼠肺组织标本。采用免疫组化方法检测HDAC6在肺组织中的表达定位,采用Western blotting定量分析肺组织中HDAC6表达水平的变化,采用比色法检测HDAC6酶活性的变化。结果 与正常组相比,哮喘组小鼠肺组织HDAC6的表达水平和酶活性均显著升高(P<0.05);正常组小鼠肺组织结构细胞中HDAC6表达极少,哮喘组肺组织气道上皮细胞、气道血管周围炎症细胞、平滑肌细胞以及肺泡腔炎症细胞中HDAC6呈明显阳性表达。结论 哮喘致病时肺组织中HDAC6的表达定位、表达水平和酶活性均发生不同程度改变。

关键词: 支气管哮喘, 组蛋白去乙酰化酶6, 气道炎症, 气道重塑

Abstract: Objective To investigate the expression pattern of histone deacetylase 6(HDAC6) in the lung tissue of chronic asthmatic mice. Methods BALB/C mice were divided into control and asthma groups(n=12 per group). Ovalbumin sensitization and excitation were used to construct the chronic asthma model. Lung tissue samples from each group were collected 24 h after the last challenge test. Immunohistochemistry was performed to determine HDAC6 localization in lung tissue. Western blotting was used to quantitatively analyze HDAC6 expression. Colorimetry was used to detect HDAC6 activity. Results Compared to the control group, the expression and activity of HDAC6 increased significantly in the lung tissue of the asthma group(P<0.05). HDAC6 expression was observed in airway epithelial cells, airway perivascular inflammatory cells, smooth muscle cells, and alveolar inflammatory cells of the asthma group, whereas it was expressed negligibly in lung tissue of the control group. Conclusion HDAC6 expression, localization, and activity in lung tissue changed to varying degrees during asthma pathogenesis.

Key words: bronchial asthma, histone deacetylase 6, airway inflammation, airway remodeling

中图分类号: 

  • R562.25
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