中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (9): 807-811.doi: 10.12007/j.issn.0258-4646.2018.09.009

• 论著 • 上一篇    下一篇

miR-26a在溃疡性结肠炎中的表达及对自噬的影响

曹勇, 王东旭, 孙妍, 杨俊, 张营, 郑长清   

  1. 中国医科大学附属盛京医院第二消化内科, 沈阳 110004
  • 收稿日期:2018-04-12 出版日期:2018-09-30 发布日期:2018-09-08
  • 通讯作者: 郑长清 E-mail:zhengchangqing88@163.com
  • 作者简介:曹勇(1980-),女,主治医师,硕士.
  • 基金资助:
    辽宁省科学技术计划(2013225303)

miR-26a Expression in Ulcerative Colitis and Its Effect on Cell Autophagy

CAO Yong, WANG Dongxu, SUN Yan, YANG Jun, ZHANG Ying, ZHENG Changqing   

  1. Department of Gastroenterology, Shengjing Hospital, China Medical University, Shenyang 110004, China
  • Received:2018-04-12 Online:2018-09-30 Published:2018-09-08

摘要: 目的 探讨miR-26a在溃疡性结肠炎中的表达及其与自噬和凋亡的关系。方法 应用实时PCR检测活动期溃疡性结肠炎患者和正常对照者组织及血清中miR-26a的表达。应用miR-26a mimics/inhibitor过表达/敲低miR-26a在Caco-2细胞中的表达,应用流式细胞技术检测凋亡情况,应用Western blotting检测相关蛋白LC3-Ⅱ/LC3-Ⅰ及p62蛋白的表达。结果 与正常对照者相比,活动期溃疡性结肠炎患者组织中miR-26a呈高表达;过表达miR-26a后Caco-2细胞凋亡增加,自噬水平降低,敲低miR-26a后Caco-2细胞凋亡减少,自噬增加。结论 活动期溃疡性结肠炎组织中miR-26a高表达,且其高表达促进细胞凋亡并抑制自噬。

关键词: 溃疡性结肠炎, 自噬, 凋亡

Abstract: Objective To determine miR-26a expression in ulcerative colitis and its relationship with autophagy and apoptosis. Methods miR-26a expression in sera of patients with active ulcerative colitis and normal controls was detected using real-time polymerase chain reaction(PCR). miR-26a mimics/inhibitor were used to upregulate/downregulate miR-26a expression. Apoptosis of Caco-2 cells was detected using flow cytometry. LC3-Ⅱ/LC3-Ⅰand p62 expression was detected by Western blotting. Results miR-26a expression in patients with active ulcerative colitis was higher than in normal controls. Furthermore, miR-26a expression level correlated positively with autophagy and negatively with apoptosis. Conclusion miR-26a is highly expressed in patients with active ulcerative colitis, which promotes apoptosis and reduces autophagy.

Key words: ulcerative colitis, autophagy, apoptosis

中图分类号: 

  • R284.2
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