中国医科大学学报

中国医科大学学报
  • 中文核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (10): 876-880.doi: 10.12007/j.issn.0258-4646.2018.10.003

• 论著 • 上一篇    下一篇

熊果酸对香烟烟雾诱导大鼠肺气肿及氧化应激的影响

林莉, 殷典贺, 韩丹, 侯刚, 尹燕, 康健, 王秋月   

  1. 中国医科大学附属第一医院呼吸与危重症医学科, 呼吸疾病研究所, 沈阳 110001
  • 收稿日期:2018-03-13 出版日期:2018-10-30 发布日期:2018-09-30
  • 通讯作者: 王秋月 E-mail:qywang2002@hotmail.com
  • 作者简介:林莉(1983-),女,主治医师,博士.
  • 基金资助:
    国家重点研发计划(2016YFC1304103);辽宁省高等学校创新团队(LT2013015)

Effects of Ursolic Acid on Cigarette Smoke-induced Emphysema and Oxidative Stress in Rats

LIN Li, YIN Dianhe, HAN Dan, HOU Gang, YIN Yan, KANG Jian, WANG Qiuyue   

  1. Institute of Respiratory Disease, Department of Respiratory and Critical Care Medicine, The First Hospital, China Medical University, Shenyang 110001, China
  • Received:2018-03-13 Online:2018-10-30 Published:2018-09-30

摘要: 目的 探讨熊果酸对香烟烟雾诱导大鼠肺气肿及肺组织氧化应激的影响。方法 选取雄性Wistar大鼠24只,平均分为空白组、模型组和熊果酸组。观察肺组织病理学改变;测定血清中抗氧化酶:超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)的活性;ELISA法检测血清中氧化产物8-异前列腺素F2α(8-iso-PGF2α)的表达;硫代巴比妥酸(TBA)法检测肺组织匀浆上清液中丙二醛(MDA)的表达,IHC法分析肺组织切片8-羟基脱氧鸟苷(8-OHdG)的表达。结果 模型组大鼠肺组织呈现明显的肺气肿改变,给予熊果酸干预后上述改变明显减轻。模型组血清中的SOD、CAT、GSH-Px活性明显低于对照组,而熊果酸组与模型组相比,SOD、CAT和GSH-Px的活性升高;模型组8-iso-PGF2α含量明显高于对照组,给予熊果酸后表达下调;此外,熊果酸能够明显下调模型组肺组织氧化应激指标MDA及8-OHdG的表达。结论 熊果酸能上调大鼠抗氧化酶,减轻肺组织氧化应激,从而减轻香烟烟雾所致大鼠肺气肿。

关键词: 熊果酸, 肺气肿, 氧化应激

Abstract: Objective To investigate the effects of ursolic acid on cigarette smoke-induced emphysema and oxidative stress in the lungs of rats. Methods Male Wistar rats were divided into the control,model,and ursolic acid groups. The pathological changes in their lung tissues were observed. The activities of the antioxidant enzymes SOD,CAT,and GSH-Px in serum were determined;the expression of 8-iso-PGF2 in serum was detected by the ELISA method. The MDA levels in the supernatant of lung tissue homogenate was detected by the TBA method. 8-OHdG expressions in lung tissue sections were determined by the IHC method. Results The lung tissues of rats in the model group showed obvious changes of emphysema. However,these changes were significantly alleviated by ursolic acid intervention. The activities of SOD,CAT,and GSH-Px in the serum of rats from the model group were significantly lower than those in the serum of rats from the control group. However,the activities of SOD,CAT,and GSH-Px were higher in the serum of rats from the ursolic acid group than those in the serum of rats from the model group. The content of 8-iso-PGF2α in rats from the model group was significantly higher;the 8-iso-PGF2α content in rats from the ursolic acid group was downregulated. In addition,ursolic acid significantly downregulated the expression of the oxidative stress indicators MDA and 8-OHdG in the lung tissues of rats from the model group. Conclusion Ursolic acid upregulates rat antioxidant enzymes and reduces oxidative stress in lung tissues,thereby alleviating cigarette smoke-induced pulmonary emphysema.

Key words: ursolic acid, emphysema, oxidant stress

中图分类号: 

  • R563.3
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