中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (10): 891-894,899.doi: 10.12007/j.issn.0258-4646.2018.10.006

• 论著 • 上一篇    下一篇

宫内发育迟缓对大鼠胰腺中Ngn3表达及内分泌细胞发育的影响

徐春卓, 辛颖   

  1. 中国医科大学附属盛京医院小儿内分泌遗传代谢科, 沈阳 110004
  • 收稿日期:2017-11-09 出版日期:2018-10-30 发布日期:2018-09-30
  • 通讯作者: 辛颖 E-mail:xin.ying168@live.cn
  • 作者简介:徐春卓(1991-),女,医师,硕士.
  • 基金资助:
    辽宁省自然科学基金(2014021037)

Effects of Intrauterine Growth Retardation on Ngn3 Expression and Endocrine Cell Development

XU Chunzhuo, XIN Ying   

  1. Department of Pediatric Endocrinology, Shengjing Hospital, China Medical University, Shenyang 110004, China
  • Received:2017-11-09 Online:2018-10-30 Published:2018-09-30

摘要: 目的 探讨宫内发育迟缓(IUGR)对大鼠胰腺中Ngn3表达及内分泌细胞发育的影响。方法 给予孕鼠孕期全程低蛋白饲料或正常饲料任意摄取喂养,将其子鼠分为IUGR组和对照组(CON组)。选取胚胎第15.5天(E15.5)、第18.5天(E18.5),新生第1天(P1)在解剖显微镜下分离胰腺组织;采用HE染色观察胰腺形态变化,用免疫组织化学染色及图像分析技术检测Ngn3蛋白表达。结果 随着胚胎发育,光镜下胰腺腺泡、导管结构逐渐聚集成β细胞并形成内外分泌部的典型结构;组织化学染色发现在胚胎期Ngn3蛋白主要表达在胰腺的导管,新生期主要表达在胰岛中。随胎龄的增长Ngn3蛋白表达量减少,与E15.5、E18.5比较,P1时Ngn3蛋白表达量下降(均P < 0.01)。IUGR组子鼠体质量明显低于CON组(P < 0.05);光镜下观察IUGR组腺泡导管、胰岛数量及面积较CON组减少;与CON组比较,IUGR组Ngn3蛋白表达减少,E15.5、E18.5时2组差异有统计学意义(P < 0.05)。结论 IUGR可使胰腺中内分泌祖细胞减少,导致内分泌细胞发育异常。

关键词: Ngn3, 宫内发育迟缓, 内分泌发育, 大鼠

Abstract: Objective To investigate changes in Ngn3 expression due to intrauterine growth retardation (IUGR)and its effect on endocrine cell development. Methods Pregnant rats were randomly fed a standard or low-protein diet,and offspring were labeled as CON or IUGR groups. Pancreatic tissues were collected at days 15.5,18.5 gestation and the newborn. Changes in pancreatic structure were detected by HE staining,and expression of the Ngn3 protein was detected by immunohistochemical staining. Results During embryonic development,the pancreatic acinar and duct gradually formed the typical endocrine and exocrine structures. The Ngn3 protein was mainly expressed in the pancreatic duct during the embryonic period and in the islets during the newborn period. Expression of the Ngn3 protein was significantly decreased (P < 0.01). Compared to the CON group,the body weights of IUGR rats were significantly lower (P < 0.05). The number and area of pancreatic acinar,duct,and islet were lower in the IUGR group. Expression of the Ngn3 protein in the pancreas decreased,and the difference was statistically significant at E15.5 and E18.5 (P < 0.05). Conclusion Pancreatic endocrine progenitor cells were reduced,which led to endocrine cell development abnormalities induced by IUGR.

Key words: Ngn3, intrauterine growth retardation, endocrine development, rat

中图分类号: 

  • R725.8
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