中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (10): 895-899.doi: 10.12007/j.issn.0258-4646.2018.10.007

• 论著 • 上一篇    下一篇

长链非编码RNA XIST对肝癌细胞增殖及侵袭的影响

柴芳, 李岩, 刘克毅, 李强   

  1. 锦州医科大学附属第一医院普外科, 辽宁锦州 121001
  • 收稿日期:2018-01-04 出版日期:2018-10-30 发布日期:2018-09-30
  • 通讯作者: 柴芳 E-mail:lyfsdyyy@163.com
  • 作者简介:柴芳(1973-),男,副主任医师,博士.
  • 基金资助:
    辽宁省自然科学基金(20170540349)

Effects of Long Non-coding RNA XIST on Proliferation and Invasion of the Hepatocellular Carcinoma

CHAI Fang, LI Yan, LIU Keyi, LI Qiang   

  1. Department of General Surgery, The First Affliated Hospital, Jinzhou Medical University, Jinzhou 121001, China
  • Received:2018-01-04 Online:2018-10-30 Published:2018-09-30

摘要: 目的 探讨长链非编码RNA X染色体失活特异转录本(XIST)对肝癌细胞增殖、侵袭的影响。方法 qRT-PCR检测正常肝细胞以及肝癌细胞系中XIST的表达,同时在肝癌细胞中过表达与敲除XIST表达,应用CCK-8与Transwell实验检测XIST对肝癌细胞增殖、侵袭与转移的影响。结果 XIST在肝癌细胞中的表达较正常肝细胞表达显著升高(P < 0.05);肝癌细胞系HepG2过表达XIST后能够促进肝癌细胞增殖、侵袭与转移;肝癌细胞SMMC-7721细胞中抑制XIST表达后肝癌细胞增殖、侵袭与转移能力受到抑制。结论 XIST参与肝癌细胞增殖、侵袭与转移。

关键词: 肝癌, X染色体失活特异转录本, 增殖, 侵袭, 转移

Abstract: Objective To investigate the effects of X inactive specific transcript (XIST)on proliferation,invasion,and metastasis of the hepatocellular carcinoma. Methods XIST expression was analyzed by qRT-PCR in normal and hepatoma cell lines. CCK-8 and Transwell assays were performed to analyze the effects of XIST on proliferation,invasion,and metastasis post-RNA interference on the overexpression of the XIST. Results The expression of XIST was higher in the hepatocellular carcinoma cell than the normal hepatocyte (P < 0.05). Therefore,XIST overexpression may promote proliferation,invasion,and metastasis of human hepatoma cells HepG2. The suppression of XIST expression in SMMC-7721 cells could inhibit the ability of proliferation,invasion,and metastasis of hepatoma cells. Conclusion XIST promotes proliferation,invasion,and metastasis in the hepatocellular carcinoma.

Key words: hepatocellular carcinoma, X inactive specific transcript, proliferation, invasion, metastasis

中图分类号: 

  • R605
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