中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (10): 924-928.doi: 10.12007/j.issn.0258-4646.2018.10.014

• 论著 • 上一篇    下一篇

miR-212抑制骨肉瘤细胞恶性生物学行为的实验研究

裴祎, 张晓晶, 郑珂, 王巍, 邱恩铎, 李盛龙   

  1. 中国医科大学肿瘤医院, 辽宁省肿瘤医院骨与软组织肿瘤科, 沈阳 110042
  • 收稿日期:2017-08-06 出版日期:2018-10-30 发布日期:2018-09-30
  • 通讯作者: 张晓晶 E-mail:zhangxiaojingwu@163.com
  • 作者简介:裴祎(1984-),男,主治医师,硕士.

Inhibitory Effect of miR-212 on the Malignant Biological Behavior of Osteosarcoma Cells

PEI Yi, ZHANG Xiaojing, ZHENG Ke, WANG Wei, QIU Enduo, LI Shenglong   

  1. Department of Bone and Soft Tissue Surgery, Cancer Hospital of China Medical University, Liaoning Cancer Hospital and Institute, Shenyang 110042, China
  • Received:2017-08-06 Online:2018-10-30 Published:2018-09-30

摘要: 目的 探讨miR-212对骨肉瘤细胞增殖和迁移的抑制机制。方法 MIRDB预测miR-212与锌指蛋白133(ZNF133)的结合位点后,利用荧光素酶报告基因实验检测miR-212对ZNF133的靶向作用。通过MTT实验和Transwell实验检测miR-212过表达或受到抑制后对MG63细胞增殖和迁移的影响。通过Western blotting实验检测miR-212对ZNF133及CDK4/6和MMP2的作用。结果 MIRDB预测发现miR-212与ZNF133的3’UTR区域具有结合位点,荧光素酶报告基因实验证明了miR-212可以直接作用于ZNF133。MTT实验表明转染miR-212后显著抑制MG63细胞增殖;miR-212受到抑制后促进MG63细胞增殖。Transwell实验发现miR-212可以抑制骨肉瘤细胞系MG63迁移。Western blotting实验证明miR-212对MG63细胞增殖迁移能力的抑制在一定程度上是通过miR-212对ZNF133及CDK4/6和MMP2的抑制实现的。结论 miR-212可以通过抑制ZNF133及相关蛋白抑制骨肉瘤细胞的增殖和迁移。

关键词: miR-212, 骨肉瘤, 锌指蛋白133, 迁移, 增殖

Abstract: Objective To investigate the mechanism by which miR-212 inhibits the proliferation and migration of osteosarcoma cells. Methods MIRDB predicts the binding site of miR-212 and zinc finger protein-133 (ZNF133),and a luciferase reporter assay confirms their relationship. The effects of miR-212 on the proliferation or migration of MG63 cells were detected by MTT assay and Transwell assay. Western blotting was used to detect the effect of miR-212 on the ZNF133,CDK4/6,and MMP2 expressions. Results MIRDB predicted that miR-212 had a binding site for the ZNF133 3'UTR region. The luciferase reporter gene experiments showed that miR-212 can directly act on ZNF133. The MTT test showed that after transfection of miR-212,MG63 cell proliferation was significantly inhibited. Transwell experiments showed that miR-212 could inhibit the migration ability of the osteosarcoma cell line MG63. Western blotting experiments proved that miR-212 inhibited the proliferation and migration of MG63 cells to some extent due to the miR-212-mediated inhibition of ZNF133,CDK4/6,and MMP2. Conclusion miR-212 can inhibit the proliferation and migration of osteosarcoma cells by inhibiting the ZNF133,CDK4/6,and MMP2 expressions.

Key words: miR-212, osteosarcoma, zinc finger protein-133, migration, proliferation

中图分类号: 

  • R738.1
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