中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (11): 989-992.doi: 10.12007/j.issn.0258-4646.2018.11.007

• 论著 • 上一篇    下一篇

间充质干细胞对慢性间质性肾炎大鼠肾小管上皮细胞表型的影响

李维, 杨园园, 姜红   

  1. 中国医科大学附属第一医院儿科, 沈阳 110001
  • 收稿日期:2018-06-26 出版日期:2018-11-30 发布日期:2018-12-06
  • 通讯作者: 李维 E-mail:liwei_vivian163@163.com
  • 作者简介:李维(1974-),女,副教授,博士.
  • 基金资助:
    辽宁省教育厅科学研究一般项目(L2015575)

Effect of Mesenchymal Stem Cells on the Phenotype of Renal Tubular Epithelial Cell in Rats with Interstitial Nephritits

LI Wei, YANG Yuanyuan, JIANG Hong   

  1. Department of Pediatric, The First Hospital, China Medical University, Shenyang 110001, China
  • Received:2018-06-26 Online:2018-11-30 Published:2018-12-06

摘要: 目的 观察慢性间质性肾炎大鼠模型肾脏病理变化,以及间充质干细胞对肾小管上皮细胞表型相关细胞因子表达的影响。方法 将30只雌性Wistar大鼠随机分为对照组、治疗组、非治疗组。对照组大鼠饮用水灌胃12周,治疗组和非治疗组大鼠关木通水煎剂灌胃12周。第12周末,治疗组经尾静脉输入间充质干细胞,非治疗组和对照组经尾静脉注射等量生理盐水。尾静脉注射后第8周末处死大鼠,留取血、尿、肾组织标本。肾组织连续切片,分别用免疫组化、Western blotting及半定量逆转录PCR(RT-PCR)方法检测细胞角蛋白18(CK18)蛋白和mRNA的表达情况。结果 与对照组和治疗组相比,非治疗组肾损伤严重、肾间质纤维化明显,而治疗组肾组织损伤减轻、纤维化明显改善。免疫组化染色显示,治疗组肾脏CK18阳性表达明显多于非治疗组,但仍低于对照组。RT-PCR检测显示,治疗组CK18 mRNA表达量明显增加,非治疗组CK18的表达明显减少甚至消失;肾脏Western blotting分析显示同样的变化趋势。结论 间充质干细胞通过上调肾小管上皮细胞CK18的表达,减少肾脏肾小管上皮细胞向肌成纤维细胞转分化,从而减轻肾组织病理损害和肾间质纤维化。

关键词: 慢性间质性肾炎, 间充质干细胞, 上皮-间充质转化

Abstract: Objective To observe the renal changes in rats with chronic interstitial nephritis,and the effect of mesenchymal stem cells (MSCs) on the expression of cytokines in renal tubular epithelial cells. Methods Thirty female Wistar rats were randomly divided into control,treatment,and non-treatment groups. The rats in the control group received gastric lavage with drinking water for 12 weeks,and those in the other two groups received gastric lavage with aristolochia decoction for 12 weeks. At the end of week 12,MSCs were injected through the caudal vein in the treatment group,and normal saline was injected in the other two groups. Eight weeks after the injection,the rats were sacrificed,and blood,urine,and kidney samples were collected. The mRNA and protein expressions of cytokeratin 18 (CK18) were detected by immunohistochemistry,RT-PCR,and Western blotting. Results Compared with the control and treatment groups,the renal injury and interstitial fibrosis in the non-treatment group were severe,while they were significantly reduced in the treatment group. Immunohistochemical staining showed that the positive expression of CK18 in the treatment group was significantly higher than that in the non-treatment group,but it was still lower than that in the control group. RT-PCR showed that the expression of CK18 mRNA in the treatment group was significantly enhanced,while it was decreased and even disappeared in the non-treatment group. The results of the Western blotting showed the same trend. Conclusion MSCs can reduce the epithelial-mesenchymal transition by upregulating the expression of CK18 in renal tubular epithelial cells,thus reducing renal damage and interstitial fibrosis.

Key words: chronic interstitial nephritis, mesenchymal stem cells, epithelial-mesenchymal transition

中图分类号: 

  • R69
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