中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (11): 1029-1031,1042.doi: 10.12007/j.issn.0258-4646.2018.11.016

• 论著 • 上一篇    下一篇

miR-489抑制黑色素瘤细胞的增殖

郭冰玉, 回蔷, 常鹏, 陶凯   

  1. 沈阳军区总医院整形外科, 沈阳 110840
  • 收稿日期:2017-04-18 出版日期:2018-11-30 发布日期:2018-12-06
  • 通讯作者: 陶凯 E-mail:luzongzhengxing@sina.com
  • 作者简介:郭冰玉(1989-),女,技师,硕士.

miR-489 Inhibits the Proliferation of Melanoma Cells

GUO Bingyu, HUI Qiang, CHANG Peng, TAO Kai   

  1. Department of Reconstructive and Plastic Surgery, The General Hospital of Shenyang Military, Shenyang 110840, China
  • Received:2017-04-18 Online:2018-11-30 Published:2018-12-06

摘要: 目的 检测miR-489对黑色素瘤细胞增殖的影响。方法 在黑色素瘤细胞A375中分别过表达或沉默miR-489后,通过MTT实验观察miR-489对细胞增殖的影响。进一步通过Western blotting和实时PCR检测miR-489对A375细胞中增殖相关蛋白CDK2和cyclin E的影响。结果 MTT检测发现,miR-489过表达后可以显著抑制A375细胞增殖,miR-489受到抑制后A375细胞的增殖受到促进。Western blotting和实时PCR检测发现,过表达miR-489可以抑制A375细胞中增殖相关蛋白CDK2和cyclin E蛋白的表达;miR-489受到抑制后蛋白表达增加。结论 miR-489对黑色素瘤细胞的增殖有一定的抑制作用,可能作为黑色素瘤诊疗的新靶点。

关键词: miR-489, 黑色素瘤, A375细胞, 增殖

Abstract: Objective To determine the effect of miR-489 on the proliferation of melanoma cells. Methods MTT assay was used to observe the effect of miR-489 on the proliferation of A375 cells after miR-489 was overexpressed or silenced. Western blotting and real-time PCR were used to determine the effect of miR-489 on the expression of CDK2 and cyclin E. Results MTT assays showed that overexpression of miR-489 significantly inhibited the proliferation of A375 cells and that silencing miR-489 promoted the proliferation. Western blotting and real-time PCR showed that overexpression of miR-489 inhibited the expression of CDK2 and cyclin E in A375 cells and that silencing miR-489 increased their expression. Conclusion miR-489 can inhibit the proliferation of melanoma cells and could be used as a new target for the diagnosis and treatment of melanoma.

Key words: miR-489, melanoma, A375 cells, proliferation

中图分类号: 

  • R739.5
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