中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (12): 1063-1066.doi: 10.12007/j.issn.0258-4646.2018.12.002

• 论著 • 上一篇    下一篇

低血糖应激大鼠血清FT3、FT4、TT4、TSH水平及垂体组织TSHβ mRNA表达的动态变化

张博1, 韩闻卿1, 李莹1,2, 杨歌1, 赵蕾1, 杜信1, 姜雅秋1   

  1. 1. 中国医科大学附属第一医院内分泌科, 中国医科大学内分泌研究所, 辽宁省内分泌疾病重点实验室, 沈阳 110001;
    2. 辽阳市中心医院神经内科, 辽宁 辽阳 111000
  • 收稿日期:2018-05-04 出版日期:2018-12-30 发布日期:2018-12-06
  • 通讯作者: 姜雅秋 E-mail:jiangyaqiu@yeah.net
  • 作者简介:张博(1990-),女,硕士研究生.
  • 基金资助:
    国家自然科学基金(81670719);中华医学会项目(13050870472)

Temporal Changes in Serum Levels of FT3,FT4,TT4,and TSH and Expression of Pituitary TSHβ mRNA in Rats after Hypoglycemic Stress

ZHANG Bo1, HAN Wenqing1, LI Ying1,2, YANG Ge1, ZHAO Lei1, DU Xin1, JIANG Yaqiu1   

  1. 1. Department of Endocrinology and Metabolism, Institute of Endocrinology, Liaoning Provincial Key Laboratory of Endocrine Diseases, The First Hospital, China Medical University, Shenyang 110001, China;
    2. Neurology Department, Liaoyang Central Hospital, Liaoyang 111000, China
  • Received:2018-05-04 Online:2018-12-30 Published:2018-12-06

摘要: 目的 探讨不同低血糖持续时间对大鼠甲状腺功能及垂体促甲状腺激素β(TSHβ)蛋白和mRNA表达水平的影响。方法 将37只8周龄雄性Sprague Dawley大鼠随机分为0 min、15 min、30 min、60 min、90 min组,通过钳夹试验建立低血糖大鼠模型。采用ELISA法测定各组大鼠血清游离三碘甲状腺原氨酸(FT3)、游离甲状腺素(FT4)、总甲状腺素(TT4)、促甲状腺激素(TSH)水平,分别采用实时荧光定量PCR和Western blotting检测各组大鼠垂体组织TSHβ mRNA和蛋白的表达。结果 低血糖应激后,15 min组血清FT3、FT4、TT4以及垂体TSHβ mRNA和蛋白表达水平均明显升高(P < 0.05)。60 min组血清FT3及垂体TSHβ蛋白表达明显下降(P < 0.05),而血清TSH明显升高(P < 0.05)。90 min组血清FT4、TT4明显下降(P < 0.05)。结论 低血糖应激可引起大鼠血清FT3、FT4、TT4、TSH水平及垂体TSHβ mRNA和蛋白表达水平先增加后减少。

关键词: 低血糖应激, 下丘脑-垂体-甲状腺轴, TSHβ mRNA, TSHβ蛋白

Abstract: Objective To investigate changes in the serum levels of free triiodothyronine (FT3),free thyroxin (FT4),total thyroxine (TT4),thyroid-stimulating hormone (TSH),and the mRNA and protein levels of thyroid-stimulating hormone β subunit (TSHβ) in rats after hypoglycemic stress. Methods Thirty-seven male Sprague Dawley rats aged 8 weeks were randomly divided into five groups (0,15,30,60,and 90 min groups) depending on the duration of persistent hypoglycemic stress. The rat model of hypoglycemia was established by the hypoglycemic clamp technique. Serum levels of FT3,FT4,TT4,and TSH were determined by immunosorbent assays. Real-time PCR and western blotting were used to detect the mRNA and protein expression levels of TSHβ,respectively. Results After hypoglycemic stress,serum levels of FT3,FT4,and TT4,and the mRNA and protein expressions of TSHβ in the pituitary gland all increased significantly in the 15 min group (P < 0.05). The serum level of FT3 and the expression of TSHβ protein decreased in the 60 min group (P < 0.05),while the serum level of TSH increased (P < 0.05). The serum levels of FT4 and TT4 decreased significantly in the 90 min group (P < 0.05). Conclusion Upon hypoglycemic stress,the serum levels of FT3,FT4,TT4,and TSH,and the pituitary mRNA and protein expression of TSHβ in rats first increased and then decreased.

Key words: hypoglycemic stress, hypothalamus-pituitary-thyroid axis, TSHβ mRNA, TSHβ protein

中图分类号: 

  • R581.9
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