中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (12): 1081-1084,1106.doi: 10.12007/j.issn.0258-4646.2018.12.006

• 论著 • 上一篇    下一篇

沉默长链非编码RNA浆细胞瘤可变易位1基因提高胃癌细胞对阿霉素的化疗敏感性

王冰1, 黄宝俊2, 解大龙3, 赵丹懿1   

  1. 1. 大连医科大学附属第二医院肿瘤一科, 辽宁 大连 116027;
    2. 中国医科大学附属第一医院肿瘤外科, 沈阳 110004;
    3. 中国医科大学基础医学院解剖学教研室, 沈阳 110122
  • 收稿日期:2018-04-05 出版日期:2018-12-06 发布日期:2018-12-06
  • 通讯作者: 赵丹懿 E-mail:danyizhao2000@163.com
  • 作者简介:王冰(1982-),女,主治医师,硕士.
  • 基金资助:
    国家自然科学基金(81272716)

Knockdown of Long Noncoding RNA Plasmacytoma Variant Translocation 1 Enhances Chemosensitivity of Gastric Cancer Cells to Adriamycin

WANG Bing1, HUANG Baojun2, XIE Dalong3, ZHAO Danyi1   

  1. 1. No.1 Ward, Department of Oncology, The Second Hospital of Dalian Medical University, Dalian 116027, China;
    2. Department of Surgical oncology, The First Hospital, China Medical University, Shenyang 110004, China;
    3. Department of Anatomy, College of Basic Medical Science, China Medical University, Shenyang 110122, China
  • Received:2018-04-05 Online:2018-12-06 Published:2018-12-06

摘要: 目的 研究长链非编码RNA浆细胞瘤可变易位1(PVT1)基因调控胃癌细胞对阿霉素化疗敏感性的作用。方法 RNA干扰靶向沉默SGC7901/ADR细胞中PVT1的表达,实时定量PCR检测沉默效果。增强CCK8法检测SGC7901/ADR细胞的增殖活力和化疗药物敏感性;碘化丙啶单染流式细胞术检测SGC7901/ADR细胞的细胞周期;Annexin V-FITC/PI双染流式细胞术检测SGC7901/ADR细胞的凋亡。结果 PVT1沉默能够显著降低阿霉素在SGC7901/ADR细胞中的半数抑制浓度。在0.5 μg/mL阿霉素的作用下,PVT1沉默能够抑制SGC7901/ADR细胞的增殖活力,阻滞细胞于G1期,并促进细胞凋亡。PVT1沉默能够提高SGC7901/ADR细胞对阿霉素的化疗敏感性,并提高阿霉素诱导的细胞毒性作用。结论 长链非编码RNA PVT1基因与胃癌的化疗耐药相关,PVT1沉默能够提高胃癌耐药细胞对阿霉素的化疗敏感性。

关键词: 长链非编码RNA, 胃癌, 浆细胞瘤可变易位1, 化学治疗

Abstract: Objective To study the effects of the long noncoding RNA plasmacytoma variant translocation 1 (PVT1) on the regulation of chemosensitivity of gastric cancer cells to adriamycin. Methods RNA interference was used to silence the expression of PVT1 in SGC7901/ADR cells. Real-time quantitative PCR was used to detect the effect of silencing. An enhanced CCK8 assay was used to detect the proliferation of PVT1-silenced SGC7901/ADR cells and their sensitivity to the chemotherapeutic drug adriamycin. Cell cycle analysis was performed using propidium iodide (PI) single dye flow cytometry. Apoptosis was detected using Annexin V-fluorescein isothiocyanate/PI double dye flow cytometry. Results Silencing of PVT1 significantly reduced the half-inhibitory concentration of adriamycin in SGC7901/ADR cells. At an adriamycin concentration of 0.5 μg/mL,PVT1 silencing inhibited SGC7901/ADR cell proliferation,blocked cells in the G1 phase,and promoted cell apoptosis. PVT1 silencing also enhanced the sensitivity of SGC7901/ADR cells to adriamycin and adriamycin-induced cytotoxicity. Conclusion Long noncoding RNA PVT1 is associated with chemoresistance in gastric cancer. PVT1 silencing can increase the chemosensitivity of drug-resistant gastric cancer cells to adriamycin.

Key words: long noncoding RNA, gastric cancer, plasmacytoma variant translocation 1, chemotherapy

中图分类号: 

  • R735.2
[1] INOKUCHI M,OTSUKI S,FUJIMORI Y,et al. Clinical significance of MET in gastric cancer[J]. World J Gastrointest Oncol,2015,7(11):317-327. DOI:10.4251/wjgo.v7.i11.317.
[2] SONG Z,WU Y,YANG J,et al. Progress in the treatment of advanced gastric cancer[J]. Tumour Biol,2017,39(7):1010428317714626. DOI:10.1177/1010428317714626.
[3] YANG W,MA J,ZHOU W,et al. Molecular mechanisms and theranostic potential of miRNAs in drug resistance of gastric cancer[J]. Expert Opin Ther Targets,2017,21(11):1063-1075. DOI:10.1080/14728222.2017.
[4] MARCHESE FP,RAIMONDI I,HUARTE M. The multidimensional mechanisms of long noncoding RNA function[J]. Genome Biol,2017,18(1):206. DOI:10.1186/s13059-017-1348-2.
[5] SUN W,YANG Y,XU C,et al. Regulatory mechanisms of long noncoding RNAs on gene expression in cancers[J]. Cancer Genet,2017, (216/217):105-110. DOI:10.1016/j.cancergen.2017.06.003.
[6] MAO H,WANG K,FENG Y,et al. Prognostic role of long non-coding RNA XIST expression in patients with solid tumors:a meta-analysis[J]. Cancer Cell Int,2018,18:34. DOI:10.1186/s12935-018-0535-x.
[7] ZHOU R,CHEN KK,ZHANG J,et al. The decade of exosomal long RNA species:an emerging cancer antagonist[J]. Mol Cancer,2018,17(1):75. DOI:10.1186/s12943-018-0823-z.
[8] CHANG Z,CUI J,SONG Y. Long noncoding RNA PVT1 promotes EMT via mediating microRNA-186 targeting of Twist1 in prostate cancer[J]. Gene,2018,654:36-42. DOI:10.1016/j.gene.2018.02.036.
[9] GUO D,WANG Y,REN K,et al. Knockdown of lncRNA PVT1 inhibits tumorigenesis in non-small-cell lung cancer by regulating miR-497 expression[J]. Exp Cell Res,2018,362(1):172-179. DOI:10.1016/j.yexcr.2017.11.014.
[10] HUANG T,LIU HW,CHEN JQ,et al. The long noncoding RNA PVT1 functions as a competing endogenous RNA by sponging miR-186 in gastric cancer[J]. Biomed Pharmacother,2017,88:302-308. DOI:10.1016/j.biopha.2017.01.049.
[11] YUAN CL,LI H,ZHU L,et al. Aberrant expression of long noncoding RNA PVT1 and its diagnostic and prognostic significance in patients with gastric cancer[J]. Neoplasma,2016,63(3):442-449. DOI:10.4149/314_150825N45.
[12] ZHANG J,ZHAO B,CHEN X,et al. Silence of long noncoding RNA NEAT1 inhibits malignant biological behaviors and chemotherapy resistance in gastric cancer[J]. Pathol Oncol Res,2018,24(1):109-113. DOI:10.1007/s12253-017-0233-3.
[13] SHANG C,GUO Y,ZHANG J,et al. Silence of long noncoding RNA UCA1 inhibits malignant proliferation and chemotherapy resistance to adriamycin in gastric cancer[J]. Cancer Chemother Pharmacol,2016,77(5):1061-1067. DOI:10.1007/s00280-016-3029-3.
[14] FANG Q,CHEN X,ZHI X. Long non-coding RNA (LncRNA) urothelial carcinoma associated 1(UCA1) increases multi-drug resistance of gastric cancer via downregulating miR-27b[J]. Med Sci Monit,2016,22:3506-3513. DOI:10.12659/MSM.900688.
[15] PING G,XIONG W,ZHANG L,et al. Silencing long noncoding RNA PVT1 inhibits tumorigenesis and cisplatin resistance of colorectal cancer[J]. Am J Transl Res,2018,10(1):138-149.
[16] LIU E,LIU Z,ZHOU Y,et al. Overexpression of long non-coding RNA PVT1 in ovarian cancer cells promotes cisplatin resistance by regulating apoptotic pathways[J]. Int J Clin Exp Med,2015,8(11):20565-20572.
[17] YOSHIDA K,TODEN S,RAVINDRANATHAN P,et al. Curcumin sensitizes pancreatic cancer cells to gemcitabine by attenuating PRC2 subunit EZH2,and the lncRNA PVT1 expression[J]. Carcinogenesis,2017,38(10):1036-1046. DOI:10.1093/carcin/bgx065.
[18] ZHANG XW,BU P,LIU L,et al. Overexpression of long non-coding RNA PVT1 in gastric cancer cells promotes the development of multidrug resistance[J]. Biochem Biophys Res Commun,2015,462(3):227-232. DOI:10.1016/j.bbrc.2015.04.121.
[19] ZHANG XW,LIU L,ZHANG XZ,et al. Kanglaite inhibits the expression of drug resistance genes through suppressing PVT1 in cisplatin-resistant gastric cancer cells[J]. Exp Ther Med,2017,14(2):1789-1794. DOI:10.3892/etm.2017.4650.
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