中国医科大学学报

中国医科大学学报
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (12): 1085-1088.doi: 10.12007/j.issn.0258-4646.2018.12.007

• 论著 • 上一篇    下一篇

强制性运动疗法结合抗抑郁治疗对脑梗死大鼠神经功能的影响

曲慧玲1, 赵传胜2   

  1. 1. 辽宁省人民医院神经内科, 沈阳 110015;
    2. 中国医科大学附属第一医院神经内科, 沈阳 110001
  • 收稿日期:2018-05-22 出版日期:2018-12-06 发布日期:2018-12-06
  • 通讯作者: 赵传胜 E-mail:cszhao@cmu.edu.cn
  • 作者简介:曲慧玲(1986-),女,主治医师,博士.
  • 基金资助:
    辽宁省重点研发计划攻关项目(2017225005)

Effects of Constraint-Induced Movement Therapy Combined with Antidepressant Treatment on Recovery after Cerebral Infarction in Rats

QU Huiling1, ZHAO Chuansheng2   

  1. 1. Department of Neurology, People's Hospital of Liaoning Province, Shenyang 110015, China;
    2. Department of Neurology, The First Hospital, China Medical University, Shenyang 110001, China
  • Received:2018-05-22 Online:2018-12-06 Published:2018-12-06

摘要: 目的 探讨强制性运动疗法(CIMT)结合抗抑郁治疗对脑梗死大鼠神经发生及运动功能的影响。方法 成年大鼠随机分为4组:假手术组(sham组)、梗死组、梗死后CIMT组(CIMT组)、梗死后CIMT结合抗抑郁治疗组(CIMT+FLUO组)。使用内皮素制作脑梗死大鼠模型。脑梗死后1周使用石膏绷带固定健侧上肢制作CIMT模型,CIMT组CIMT进行3周。CIMT+FLUO组脑梗死后1周,大鼠氟西汀(16 mg/kg)口服,持续3周。使用免疫荧光染色检测DCX和BrdU双染阳性细胞表达,应用梯形平衡木测试大鼠的肢体运动功能。结果 与Sham组比较,脑梗死组大鼠侧脑室下区新生神经细胞明显增多(P < 0.01)。与脑梗死组比较,CIMT组侧脑室下区新生神经细胞进一步增多(P < 0.01);同时运动功能恢复(P < 0.05)。而CIMT+FLUO组与CIMT组比较,新生神经细胞及运动功能恢复没有统计学差异(P > 0.05)。结论 CIMT能够促进大鼠脑梗死后神经发生及运动功能恢复,联合使用抗抑郁治疗未出现明确的促进作用。

关键词: 神经发生, 脑梗死, 强制性运动疗法, 氟西汀, 大鼠

Abstract: Objective To determine whether constraint-induced movement therapy (CIMT) combined with antidepressant treatment could influence neurogenesis and recovery after cerebral infarction in rats. Methods Adult rats were divided into a sham group,an infarction group,an infarction treated with CIMT group (CIMT group),and an infarction treated with CIMT+fluoxetine (FLUO) group (CIMT+FLUO group). Infarction was induced with endothelin-1 (ET-1). The healthy forelimbs of rats in the CIMT group were restrained in plaster casts. Rats in the CIMT+FLUO group consumed water with fluoxetine for 3 weeks. The expression of doublecortin and 3,3'-diaminobenzidine was measured using immunohistochemistry techniques,and motor function was evaluated with a beam walking test. Results CIMT enhanced the proliferation of neuroblasts (P < 0.01) and behavioral recovery (P < 0.05). CIMT combined with antidepressant treatment in CIMT+FLUO group had no effect on neurogenesis and recovery compared with CIMT group (all P > 0.05). Conclusion CIMT could enhance the neurogenesis and recovery after cerebral infarction,and there are nofurther promotion on CIMT combined with antidepressant treatment.

Key words: neurogenesis, cerebral infarction, constraint-induced movement therapy, fluoxetine, rat

中图分类号: 

  • R741
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