中国医科大学学报

中国医科大学学报
  • 中文核心期刊
  • 中国科技核心期刊
  • 中国高校百佳科技期刊
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (12): 1089-1092.doi: 10.12007/j.issn.0258-4646.2018.12.008

• 论著 • 上一篇    下一篇

脂肪源性干细胞:甲状旁腺样细胞自体移植的新来源

张平1, 张浩1, 董文武1, 王志宏1, 秦元1, 董齐1,2   

  1. 1. 中国医科大学附属第一医院甲状腺外科, 沈阳 110001;
    2. 中国医科大学人民医院普通外科, 沈阳 110016
  • 收稿日期:2018-09-04 出版日期:2018-12-06 发布日期:2018-12-06
  • 通讯作者: 董齐 E-mail:dongqi@cmu.edu.cn
  • 作者简介:张平(1970-),男,副主任医师,硕士.
  • 基金资助:
    辽宁省自然科学基金(2015020536)

Adipose-Derived Stem Cells: a Novel Source of Parathyroid Like Cells for Autologous Transplantation

ZHANG Ping1, ZHANG Hao1, DONG Wenwu1, WANG Zhihong1, QIN Yuan1, DONG Qi1,2   

  1. 1. Department of Thyroid Surgery, The First Hospital, China Medical University, Shenyang 110001, China;
    2. Department of General Surgery, The People's Hospital, China Medical University, Shenyang 110016, China
  • Received:2018-09-04 Online:2018-12-06 Published:2018-12-06

摘要: 目的 研究脂肪源性干细胞(ADSCs)分化成甲状旁腺样细胞的方法,为治愈甲状腺手术后甲状旁腺功能减退症提供新的理论与实验依据。方法 切取SD大鼠腹股沟区脂肪,经过处理培养后,通过成脂和成骨诱导实验进行ADSCs鉴定;应用Shh和激活素A(Activin A)刺激ADSCs,通过对甲状旁腺激素(PTH)的检测,证明ADSCs向甲状旁腺样细胞分化是否成功。结果 大鼠脂肪细胞分离出ADSCs,100 ng/mL Shh加75 ng/mL Activin A诱导ADSCs 21 d,培养基中PTH明显增加,证明ADSCs成功分化成甲状旁腺样细胞。结论 ADSCs经Shh和Activin A诱导后可分化成甲状旁腺样细胞。

关键词: 甲状旁腺, 脂肪源性干细胞, 自体移植

Abstract: Objective To evaluate the method of differentiation of adipose-derived stem cells (ADSCs) into parathyroid like cells to provide a new theoretical and experimental basis for the cure of hypoparathyroidism after thyroidectomy. Methods SD rats were used,and the fat of the inguinal area was cut. After treatment and culture,ADSCs were identified by the induction of lipid and osteogenesis. ADSCs were induced by Shh and Activin A. ADSC differentiation into parathyroid like cells was confirmed by the detection of parathyroid hormone (PTH). Results ADSCs were successfully isolated from rat adipocytes. ADSCs were induced using 100 ng/mL Shh +75 ng/mL Activin A for 21 days. PTH expression in the culture medium increased significantly,which proved that ADSCs had successfully differentiated into parathyroid like cells. Conclusion ADSCs can be differentiated into parathyroid like cells,which will lead to a novel cure for hypoparathyroidism.

Key words: parathyroid gland, adipose-derived stem cell, autologous transplantation

中图分类号: 

  • R6
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