中国医科大学学报

中国医科大学学报
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  • 中国高校百佳科技期刊
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中国医科大学学报 ›› 2018, Vol. 47 ›› Issue (12): 1098-1101.doi: 10.12007/j.issn.0258-4646.2018.12.010

• 论著 • 上一篇    下一篇

不同年龄组女性胚胎的非整倍性分析

马会平1, 周晓航1, 梁悦2, 刘丽英1   

  1. 1. 沈阳市妇婴医院生殖中心, 沈阳 110011;
    2. 辽宁省计划生育科学研究院临床检验中心, 沈阳 110031
  • 收稿日期:2018-04-03 出版日期:2018-12-06 发布日期:2018-12-06
  • 通讯作者: 刘丽英 E-mail:llying007@sohu.com
  • 作者简介:马会平(1982-),女,主管检验师,硕士.
  • 基金资助:
    辽宁省自然科学基金(20170540836);沈阳市科技计划(F15-159-1-00)

Aneuploidy in Embryos from Women of Different Age Groups

MA Huiping1, ZHOU Xiaohang1, LIANG Yue2, LIU Liying1   

  1. 1. Reproductive Medicine Center, Shenyang Women's and Children's Hospital, Shenyang 110011, China;
    2. Experimental Research Center, Liaoning Research Institute of Family Planning, Shenyang 110031, China
  • Received:2018-04-03 Online:2018-12-06 Published:2018-12-06

摘要: 目的 分析不同年龄组女性胚胎的非整倍性情况。方法 收集沈阳市妇婴医院生殖中心患者捐赠用于科学研究的胚胎,根据女方年龄将胚胎分为高龄组(A组,年龄>35岁)和低龄组(C组,年龄 ≤ 35岁)。将胚胎培养至囊胚期,取囊胚滋养层细胞活检,检测胚胎的非整倍性。结果 共收集捐赠胚胎149枚,其中A组胚胎82枚,C组胚胎67枚;A组活检胚胎48枚,染色体异常32例;C组活检胚胎50枚,染色体异常22例。结论 高龄女性胚胎染色体异常率高于低龄女性,为获得健康胚胎,建议高龄女性在行体外受精-胚胎移植(IVF-ET)时,结合植入前遗传学筛查(PGS),形态学不能作为胚胎筛选的唯一标准。

关键词: 高龄, 囊胚期活检, 胚胎植入前遗传学筛查, 下一代测序技术

Abstract: Objective To analyze aneuploidy in embryos from women of different age groups. Methods Embryos donated for scientific research by patients in the Reproductive Center of Shenyang Women's and Children's Hospital were examined. The embryos were divided into group A from the advanced age group (> 35 years old) and group C from the younger group (≤ 35 years old). After the embryos were cultured to blastocyst stage,trophoblast cells were collected,and aneuploidy was evaluated. Results In group A,32 of 48 embryos had a chromosomal abnormality;in group C,22 of 50 embryos had a chromosomal abnormality. Conclusion The rate of chromosomal abnormalities was higher in embryos from the advanced age group than in those from the younger group. To obtain healthy embryos,women in the advanced age group should undergo in vitro fertilization and embryo transfer in addition to preimplantation genetic screening. Morphology should not be the only criterion for embryo screening.

Key words: advanced age, blastocyst biopsy, preimplantation genetic screening, next-generation sequencing

中图分类号: 

  • R715.5
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